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2.3.1.286: protein acetyllysine N-acetyltransferase

This is an abbreviated version!
For detailed information about protein acetyllysine N-acetyltransferase, go to the full flat file.

Word Map on EC 2.3.1.286

Reaction

[protein]-N6-acetyl-L-lysine
+
NAD+
 +
H2O
=
[protein]-L-lysine
 +
2''-O-acetyl-ADP-D-ribose
 +
nicotinamide

Synonyms

Af2Sir2, Clr3, CobB, HDAC, histone deacetylase, Hst1, HST2, Hst2p, KAT, More, NAD+-dependent protein deacetylase, NAD-dependent histone deacetylase, NAD-dependent protein deacetylase, nicotinamide adenine dinucleotide-dependent protein deacetylase, patZ, peptidyl-lysine N-acetyltransferase, phnO, protein lysine acetyltransferase, protein lysine deacetylase, Rv1151c, silent information regulator 2, Sir-2, Sir2, SIR2-Af1, Sir2-Af2, Sir2A, Sir2Af2, Sir2alpha, Sir2p, SIRT1, SIRT2, SIRT3, SIRT5, sirtuin, sirtuin 1, sirtuin 3, sirtuin-2 deacetylase, YfiQ, YiaC, YjaB

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.286 protein acetyllysine N-acetyltransferase

Engineering

Engineering on EC 2.3.1.286 - protein acetyllysine N-acetyltransferase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E186A
-
inactive
H135A
-
inactive
Q115A
-
the mutant shows very weak deacetylation activity compared to the wild type enzyme
S36A
-
inactive
E78A
site-directed mutagenesis, mutation of the conserved catalytic amino acid prevents PhnO-dependent acetylation
F70A
site-directed mutagenesis, mutation of the conserved catalytic amino acid reduces YiaC-dependent acetylation compared to wild-type. Overexpression of YiaC YF70A inhibits cell migration similarly to overexpression of wild-type YiaC
Y115A
site-directed mutagenesis, mutation of the conserved catalytic amino acid prevents YiaC-dependent acetylation. Mutant YiaC Y115A is unable to inhibit cell migration in contrast to the wild-type enzyme
Y117A
site-directed mutagenesis, mutation of the conserved catalytic amino acid reduces YjaB-dependent acetylation compared to wild-type
Y117F
site-directed mutagenesis, mutation of the conserved catalytic amino acid does not alter YjaB-dependent acetylation compared to wild-type
Y128A
site-directed mutagenesis, mutation of the conserved catalytic amino acid prevents PhnO-dependent acetylation
H248Y
inactive
D118N
the mutant retains approximately 13% of the activity of the wild type protein
H135A
-
the kcat/Km profile for the mutant exhibits no pH dependence over the range tested (pH 5.4-9.4), with kcat/Km values 2 orders of magnitude lower than wild type at high pH values
I117F
the mutant is nearly as active as the wild type protein (about 75% activity)
I117L
the mutant retains approximately 25% of the activity of the wild type protein
I117V
the mutant is nearly as active as the wild type protein (about 65% activity)
D101N
additional information