1.14.14.114: amorpha-4,11-diene 12-monooxygenase
This is an abbreviated version!
For detailed information about amorpha-4,11-diene 12-monooxygenase, go to the full flat file.
Word Map on EC 1.14.14.114
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1.14.14.114
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annua
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artemisia
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malaria
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artemisinic
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trichomes
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sesquiterpene
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dihydroartemisinic
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lactone
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glandular
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anti-malarial
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trichome-specific
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farnesyl
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endoperoxide
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sesquiterpenoids
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artemisinin-based
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one-hybrid
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arteannuin
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amorphadiene
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asteraceae
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synthesis
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t-shaped
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jasmonate-responsive
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12-hydroxylase
- 1.14.14.114
- annua
- artemisia
-
malaria
-
artemisinic
- trichomes
-
sesquiterpene
-
dihydroartemisinic
- lactone
-
glandular
-
anti-malarial
-
trichome-specific
-
farnesyl
-
endoperoxide
- sesquiterpenoids
-
artemisinin-based
-
one-hybrid
-
arteannuin
-
amorphadiene
- asteraceae
- synthesis
-
t-shaped
-
jasmonate-responsive
-
12-hydroxylase
Reaction
+ 3 O2 + 3 [reduced NADPH-hemoprotein reductase] = + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
Synonyms
ALDH1, amorpha-4,11-diene C-12 oxidase, amorpha-4,11-diene monooxygenase, CYP71AV1, cytochrome P450 monooxygenase 71 AV1, EC 1.14.13.158
ECTree
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Engineering
Engineering on EC 1.14.14.114 - amorpha-4,11-diene 12-monooxygenase
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additional information
construction of enzyme knockout mutant cyp71av1-1, phenotype, overview
additional information
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construction of enzyme knockout mutant cyp71av1-1, phenotype, overview
additional information
Saccharomyces cerevisiae expressing the native Artemisia annua cytochrome P450 monooxygenase (CYP71AV1) and artemisinic aldehyde D11(13) reductase (DBR2) is used as a whole-cell biocatalyst to produce the immediate artemisinin precursor, dihydroartemisinic acid (DHAA). Introducing artemisinic aldehyde dehydrogenase (ALDH1) from Artemisia annua, which recycles NADPH, results in a significant enhancement in artemisinate titer. Method optimization and evaluation, host screening and cofactor engineering, overview. The co-expression of DBR2 and modulation of abiotic conditions result in a higher yield. Optimal condition are 80 OD yeast cells in 0.05 ml reaction at pH 8.0. Growing the yeast cells at 20°C in galactose medium increases the dihydroartemisinate titer by 50%