1.14.14.114: amorpha-4,11-diene 12-monooxygenase
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For detailed information about amorpha-4,11-diene 12-monooxygenase, go to the full flat file.
Word Map on EC 1.14.14.114
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1.14.14.114
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annua
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artemisia
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malaria
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artemisinic
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trichomes
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sesquiterpene
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dihydroartemisinic
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lactone
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glandular
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anti-malarial
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trichome-specific
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farnesyl
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endoperoxide
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sesquiterpenoids
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artemisinin-based
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one-hybrid
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arteannuin
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amorphadiene
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asteraceae
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synthesis
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t-shaped
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jasmonate-responsive
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12-hydroxylase
- 1.14.14.114
- annua
- artemisia
-
malaria
-
artemisinic
- trichomes
-
sesquiterpene
-
dihydroartemisinic
- lactone
-
glandular
-
anti-malarial
-
trichome-specific
-
farnesyl
-
endoperoxide
- sesquiterpenoids
-
artemisinin-based
-
one-hybrid
-
arteannuin
-
amorphadiene
- asteraceae
- synthesis
-
t-shaped
-
jasmonate-responsive
-
12-hydroxylase
Reaction
+ 3 O2 + 3 [reduced NADPH-hemoprotein reductase] = + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
Synonyms
ALDH1, amorpha-4,11-diene C-12 oxidase, amorpha-4,11-diene monooxygenase, CYP71AV1, cytochrome P450 monooxygenase 71 AV1, EC 1.14.13.158
ECTree
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synthesis
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heterologous expression of Artemisia annua amorphadiene synthase and CYP71AV1 in tobacco lead to the accumulation of amorphadiene and artemisinic alcohol, but not artemisinic acid. Additional expression of artemisinic aldehyde DELTA11(13) double-bond reductase DBR2 with or without aldehyde dehydrogenase 1 leads to the additional accumulation dihydroartemisinic alcohol. Results and suggest that amorphane sesquiterpenoid aldehydes are formed, but conditions in the transgenic tobacco cells favour reduction to alcohols rather than oxidation to acids
synthesis
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selection of self-pollinated plants for artemisin biosynthesis. Selfpollinated F2 plants selected are grown under optimized growth conditions. The leaves on the main stems exhibit obvious morphological changes, from indented single leaves to odd, pinnately compound leaves. Leaves and flowers form glandular and T-shaped trichomes on their surfaces. The glandular trichome densities increases from the bottom to the top leaves. Leaves, flowers, and young seedlings of F2 plants produce artemisinin. In leaves, the levels of artemisinin increase from the bottom to the top of the plants, showing a positive correlation to the density increase of glandular trichomes. Progeny of selfpollinated plants expresses the amorpha-4, 11-diene synthase and cytochrome P450 monooxygenase 71 AV1genes, which are involved in artemisinin biosynthesis in leaves and flowers
synthesis
Saccharomyces cerevisiae expressing the native Artemisia annua cytochrome P450 monooxygenase (CYP71AV1) and artemisinic aldehyde D11(13) reductase (DBR2) is used as a whole-cell biocatalyst to produce the immediate artemisinin precursor, dihydroartemisinic acid (DHAA)