2.3.1.269: apolipoprotein N-acyltransferase
This is an abbreviated version!
For detailed information about apolipoprotein N-acyltransferase, go to the full flat file.
Word Map on EC 2.3.1.269
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2.3.1.269
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apolipoproteins
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n-acylation
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triacylated
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membrane-embedded
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prolipoprotein
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diacylglyceryl
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phosphatidylglycerol
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acyl-enzyme
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braun
- 2.3.1.269
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apolipoproteins
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n-acylation
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triacylated
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membrane-embedded
- prolipoprotein
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diacylglyceryl
- phosphatidylglycerol
- acyl-enzyme
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braun
Reaction
Synonyms
ALP N-acyltransferase, apolipoprotein N-acyl transferase, BCG_2070c, lnt, LntMs, NMB0713, Ppm1Tb
ECTree
2.3.1.269 apolipoprotein N-acyltransferaseAdvanced search results
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results (Substrates Products
Substrates Products on EC 2.3.1.269 - apolipoprotein N-acyltransferase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
1-alkyne-2-acyl-phosphatidylethanolamine + [FSL-1 apolipopeptide]-S-1,2-diacyl-sn-glyceryl-L-cysteine
1-lyso-2-acyl-phosphatidylethanolamine + [FSL-1 lipopeptide]-N-alkyne-S-1,2-diacyl-sn-glyceryl-L-cysteine
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-
-
?
1-palmitoyl alkyne-2-oleoyl-sn-glycero-3-phosphatidylethanolamine + [FSL-1 apolipopeptide]-S-1,2-diacyl-sn-glyceryl-L-cysteine
1-lyso-2-oleoyl-sn-glycero-3-phosphatidylethanolamine + [FSL-1 lipopeptide]-N-palmitoyl alkyne-S-1,2-diacyl-sn-glyceryl-L-cysteine
-
-
-
?
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine + [apolipoprotein Pal]-S-1,2-diacyl-sn-glyceryl-L-cysteine
1-lyso-2-oleoyl-sn-glycero-3-phosphoethanolamine + [lipoprotein Pal]-N-palmitoyl-S-1,2-diacyl-sn-glyceryl-L-cysteine
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-
-
-
?
1-palmitoyl-2-vaccenoyl-sn-glycero-3-phosphoethanolamine + an [apolipoprotein]-S-1,2-diacyl-sn-glyceryl-L-cysteine
? + a [lipoprotein]-N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteine
-
-
-
-
?
a phosphoglycerolipid + an [apolipoprotein]-S-1,2-diacyl-sn-glyceryl-L-cysteine
a 1-lyso-phosphoglycerolipid + a [lipoprotein]-N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteine
a phosphoglycerolipid + [apolipoprotein LolC]-S-1,2-diacyl-sn-glyceryl-L-cysteine
a 1-lyso-phosphoglycerolipid + [lipoprotein LolC]-N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteine
-
-
-
-
?
a phosphoglycerolipid + [apolipoprotein LolE]-S-1,2-diacyl-sn-glyceryl-L-cysteine
a 1-lyso-phosphoglycerolipid + [lipoprotein LolE]-N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteine
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-
-
-
?
a phosphoglycerolipid + [apolipoprotein Lppx]-S-1,2-diacyl-sn-glyceryl-L-cysteine
a 1-lyso-phosphoglycerolipid + [lipoprotein Lppx]-N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteine
a phosphoglycerolipid + [apolipoprotein Lpp]-S-1,2-diacyl-sn-glyceryl-L-cysteine
a 1-lyso-phosphoglycerolipid + [lipoprotein Lpp]-N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteine
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-
-
-
?
a phosphoglycerolipid + [apolipoprotein MalE]-S-1,2-diacyl-sn-glyceryl-L-cysteine
a 1-lyso-phosphoglycerolipid + [lipoprotein MalE]-N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteine
-
-
-
-
?
a phosphoglycerolipid + [apolipoprotein NlpD]-S-1,2-diacyl-sn-glyceryl-L-cysteine
a 1-lyso-phosphoglycerolipid + [lipoprotein NlpD]-N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteine
-
-
-
-
?
a phosphoglycerolipid + [apolipoprotein OmpA]-S-1,2-diacyl-sn-glyceryl-L-cysteine
a 1-lyso-phosphoglycerolipid + [lipoprotein OmpA]-N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteine
-
-
-
-
?
a phosphoglycerolipid + [apolipoprotein Pal]-S-1,2-diacyl-sn-glyceryl-L-cysteine
a 1-lyso-phosphoglycerolipid + [lipoprotein Pal]-N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteine
-
-
-
-
?
cardiolipin + [apolipoprotein]-S-1,2-diacyl-sn-glyceryl-L-cysteine
1-lyso-cardiolipin + [lipoprotein]-N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteine
-
-
-
?
phosphatidylethanolamine + [apolipoprotein FSL-1]-S-1,2-diacyl-sn-glyceryl-L-cysteine
1-lyso-phosphatidylethanolamine + [lipoprotein FSL-1]-N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteine
phosphatidylethanolamine + [apolipoprotein]-S-1,2-diacyl-sn-glyceryl-L-cysteine
1-lyso-phosphatidylethanolamine + [lipoprotein]-N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteine
phosphatidylethanolamine+ [apolipeptide FSL-1]-S-1,2-diacyl-sn-glyceryl-L-cysteine
?
-
-
-
-
?
phosphatidylglycerol + [apolipoprotein]-S-1,2-diacyl-sn-glyceryl-L-cysteine
1-lyso-phosphatidylglycerol + [lipoprotein]-N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteine
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-
-
?
a phosphoglycerolipid + an [apolipoprotein]-S-1,2-diacyl-sn-glyceryl-L-cysteine
a 1-lyso-phosphoglycerolipid + a [lipoprotein]-N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteine
-
-
-
?
a phosphoglycerolipid + an [apolipoprotein]-S-1,2-diacyl-sn-glyceryl-L-cysteine
a 1-lyso-phosphoglycerolipid + a [lipoprotein]-N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteine
Acinetobacter baumannii AB5075-UW
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-
-
?
a phosphoglycerolipid + an [apolipoprotein]-S-1,2-diacyl-sn-glyceryl-L-cysteine
a 1-lyso-phosphoglycerolipid + a [lipoprotein]-N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteine
-
-
-
?
a phosphoglycerolipid + an [apolipoprotein]-S-1,2-diacyl-sn-glyceryl-L-cysteine
a 1-lyso-phosphoglycerolipid + a [lipoprotein]-N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteine
-
-
-
?
a phosphoglycerolipid + an [apolipoprotein]-S-1,2-diacyl-sn-glyceryl-L-cysteine
a 1-lyso-phosphoglycerolipid + a [lipoprotein]-N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteine
-
-
-
?
a phosphoglycerolipid + an [apolipoprotein]-S-1,2-diacyl-sn-glyceryl-L-cysteine
a 1-lyso-phosphoglycerolipid + a [lipoprotein]-N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteine
-
-
-
-
?
a phosphoglycerolipid + an [apolipoprotein]-S-1,2-diacyl-sn-glyceryl-L-cysteine
a 1-lyso-phosphoglycerolipid + a [lipoprotein]-N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteine
-
-
-
?
a phosphoglycerolipid + an [apolipoprotein]-S-1,2-diacyl-sn-glyceryl-L-cysteine
a 1-lyso-phosphoglycerolipid + a [lipoprotein]-N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteine
-
-
-
-
?
a phosphoglycerolipid + an [apolipoprotein]-S-1,2-diacyl-sn-glyceryl-L-cysteine
a 1-lyso-phosphoglycerolipid + a [lipoprotein]-N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteine
-
-
-
?
a phosphoglycerolipid + an [apolipoprotein]-S-1,2-diacyl-sn-glyceryl-L-cysteine
a 1-lyso-phosphoglycerolipid + a [lipoprotein]-N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteine
Lnt can use all available phospholipids such as phosphatidylglycerol (PG), phosphatidylethanolamine (PE), and cardiolipin (CL) as acyl donors. The preferred substrate is PE
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-
?
a phosphoglycerolipid + an [apolipoprotein]-S-1,2-diacyl-sn-glyceryl-L-cysteine
a 1-lyso-phosphoglycerolipid + a [lipoprotein]-N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteine
-
-
-
-
?
a phosphoglycerolipid + an [apolipoprotein]-S-1,2-diacyl-sn-glyceryl-L-cysteine
a 1-lyso-phosphoglycerolipid + a [lipoprotein]-N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteine
-
-
-
?
a phosphoglycerolipid + an [apolipoprotein]-S-1,2-diacyl-sn-glyceryl-L-cysteine
a 1-lyso-phosphoglycerolipid + a [lipoprotein]-N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteine
-
-
-
-
?
a phosphoglycerolipid + an [apolipoprotein]-S-1,2-diacyl-sn-glyceryl-L-cysteine
a 1-lyso-phosphoglycerolipid + a [lipoprotein]-N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteine
-
-
-
-
?
a phosphoglycerolipid + an [apolipoprotein]-S-1,2-diacyl-sn-glyceryl-L-cysteine
a 1-lyso-phosphoglycerolipid + a [lipoprotein]-N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteine
-
-
-
-
?
a phosphoglycerolipid + [apolipoprotein Lppx]-S-1,2-diacyl-sn-glyceryl-L-cysteine
a 1-lyso-phosphoglycerolipid + [lipoprotein Lppx]-N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteine
-
-
-
-
?
a phosphoglycerolipid + [apolipoprotein Lppx]-S-1,2-diacyl-sn-glyceryl-L-cysteine
a 1-lyso-phosphoglycerolipid + [lipoprotein Lppx]-N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteine
Mycobacterium tuberculosis Rv2051c
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-
-
-
?
a phosphoglycerolipid + [factor H binding protein]-S-1,2-diacyl-sn-glyceryl-L-cysteine
?
-
-
-
-
?
a phosphoglycerolipid + [factor H binding protein]-S-1,2-diacyl-sn-glyceryl-L-cysteine
?
-
-
-
-
?
phosphatidylethanolamine + [apolipoprotein FSL-1]-S-1,2-diacyl-sn-glyceryl-L-cysteine
1-lyso-phosphatidylethanolamine + [lipoprotein FSL-1]-N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteine
-
-
-
?
phosphatidylethanolamine + [apolipoprotein FSL-1]-S-1,2-diacyl-sn-glyceryl-L-cysteine
1-lyso-phosphatidylethanolamine + [lipoprotein FSL-1]-N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteine
-
-
-
?
phosphatidylethanolamine + [apolipoprotein]-S-1,2-diacyl-sn-glyceryl-L-cysteine
1-lyso-phosphatidylethanolamine + [lipoprotein]-N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteine
-
-
-
?
phosphatidylethanolamine + [apolipoprotein]-S-1,2-diacyl-sn-glyceryl-L-cysteine
1-lyso-phosphatidylethanolamine + [lipoprotein]-N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteine
-
-
-
-
?
additional information
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in vivo activity assay is performed using Para-lntEc Escherichia coli strain PAP8504
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additional information
?
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in vivo activity assay is performed using Para-lntEc Escherichia coli strain PAP8504
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additional information
?
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enzyme Lnt uses apolipoprotein (S-diacylglyceryl protein) as protein substrate in the reaction. Lnt catalyzes a two-step reaction via a ping-pong mechanism, whereby in the first step, a stable thioester acyl-enzyme intermediate is formed upon hydrolysis of phospholipid. 1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylethanolamine (POPE) is the preferred substrate for Lnt. When the lysophospholipid by-product is released, N-acyl transfer onto apolipoprotein occurs in the second step of the reaction. The enzyme is unable to catalyze the N-acyl transferase reaction in the presence of POPE and no effect is observed with PE-biotin. Various FSL-1 peptide substrates are N-acylated by Lnt in vitro. N-acyltransferase activity of Lnt is monitored as a shift in migration of the lipopeptide FSL-1 conjugated either with biotin or fluorescein, and detection of Lnt activity by fluorescence spectroscopy, method evaluation and optimization, overview
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additional information
?
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the short loop containing W237 might help to locally stabilize the N-terminal tail from the opposite side to allow transfer of the palmitoylate from the C387 to the apolipoprotein to produce the final mature lipoprotein
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additional information
?
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the short loop containing W237 might help to locally stabilize the N-terminal tail from the opposite side to allow transfer of the palmitoylate from the C387 to the apolipoprotein to produce the final mature lipoprotein
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additional information
?
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the substrate for N-acylation is a C16 fatty acid, whereas the two fatty acids of the diacylglycerol residue are identified as C16 and C19:0 fatty acid, the latter most likely tuberculostearic acid
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?
additional information
?
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Mycobacterium tuberculosis Rv2051c
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the substrate for N-acylation is a C16 fatty acid, whereas the two fatty acids of the diacylglycerol residue are identified as C16 and C19:0 fatty acid, the latter most likely tuberculostearic acid
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-
?
