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2.3.1.269: apolipoprotein N-acyltransferase

This is an abbreviated version!
For detailed information about apolipoprotein N-acyltransferase, go to the full flat file.

Word Map on EC 2.3.1.269

Reaction

a phosphoglycerolipid
+
an [apolipoprotein]-S-1,2-diacyl-sn-glyceryl-L-cysteine
=
a 1-lyso-phosphoglycerolipid
 +
a [lipoprotein]-N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteine

Synonyms

ALP N-acyltransferase, apolipoprotein N-acyl transferase, BCG_2070c, lnt, LntMs, NMB0713, Ppm1Tb

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.269 apolipoprotein N-acyltransferase

Cloned

Cloned on EC 2.3.1.269 - apolipoprotein N-acyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli C41(DE3) cells
expressed in Mycobacterium smegmatis
-
gene lnt, recombinant expression of C-terminally His8-tagged enzyme in Escherichia coli strain C41(DE3)
gene lnt, recombinant expression of His-tagged wild-type enzyme in Escherichia coli strain C41(DE3), the His-tagged selenomethionine (Se-Met) labeled Lnt is expressed in Escherichia coli methionine-auxotrophy strain B834 (DE3), recombinant expression of enzyme point mutants in Escherichia coli DELTAlnt knockout strain for complementation studies
gene lnt, recombinant overexpression of Strep-tagged wild-type and mutant enzymes in Escherichia coli