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2.3.1.269: apolipoprotein N-acyltransferase

This is an abbreviated version!
For detailed information about apolipoprotein N-acyltransferase, go to the full flat file.

Word Map on EC 2.3.1.269

Reaction

a phosphoglycerolipid
+
an [apolipoprotein]-S-1,2-diacyl-sn-glyceryl-L-cysteine
=
a 1-lyso-phosphoglycerolipid
 +
a [lipoprotein]-N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteine

Synonyms

ALP N-acyltransferase, apolipoprotein N-acyl transferase, BCG_2070c, lnt, LntMs, NMB0713, Ppm1Tb

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.269 apolipoprotein N-acyltransferase

Purification

Purification on EC 2.3.1.269 - apolipoprotein N-acyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-NTA column chromatography and Superdex 200 gel filtration
recombinant His-tagged wild-type enzyme from Escherichia coli strain C41(DE3) and His-tagged Se-Met-labeled Lnt from Escherichia coli strain B834 (DE3), both by nickel affinity chromatography, desalting gel filtration, ion exchange chromatography, and ultrafiltration and gel filtration
recombinant His8-tagged enzyme from Escherichia coli strain C41(DE3) membrane fraction by nickel affinity chromatography, ultrafiltration, gel filtration, and again ultrafiltration
recombinant Strep-tagged wild-type and mutant enzymes from Escherichia coli by affinity chromatography and gel filtration
Strep-Tactin column chromatography
-