2.3.1.269: apolipoprotein N-acyltransferase

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For detailed information about apolipoprotein N-acyltransferase, go to the full flat file.

Reaction

Synonyms

ALP N-acyltransferase, apolipoprotein N-acyl transferase, BCG_2070c, lnt, LntMs, NMB0713, Ppm1Tb

ECTree

     2 Transferases

         2.3 Acyltransferases

             2.3.1 Transferring groups other than aminoacyl groups

                2.3.1.269 apolipoprotein N-acyltransferase

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Results	in table
4	Activating Compound
27185	AA Sequence
6	Cloned(Commentary)
5	Crystallization (Commentary)
24	General Information
7	Inhibitors
16	Localization
1	Metals/Ions
23	Natural Substrates/ Products (Substrates)
27	Organism
1	Pathway
2	PDB ID
6	pH Optimum
37	Protein Variants
6	Purification (Commentary)
1	Reaction
26	Reference
51	Substrates and Products (Substrate)
3	Subunits
41	Synonyms
1	Systematic Name
5	Temperature Optimum [°C]

Crystallization

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Crystallization on EC 2.3.1.269 - apolipoprotein N-acyltransferase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

purified recombinant C-terminally His8-tagged enzyme, 15 mg/ml protein in 25 mM Tris, pH 8.0, 100 mM NaCl, 5% glycerol, 0.5 mM TCEP, and 0.02% DDM, is mixed with 25% PEG 2000 MME, 100 mM Na cacodylate, pH 6.4, and 40 mM MgCl2 with a drop ratio of 1:1.75 protein:precipitate, 18°C, 2 weeks. For the N-terminally cleaved detagged enzyme, 36% PEG 200, 400 mM ammonium phosphate dibasic, and 100 mM HEPES, pH 7.2, at 10°C for 3 weeks, are used. X-ray diffraction structure determination and analysis at 3.1-3.5 A resolution, modeling

Escherichia coli

P23930

758353

purified recombinant wild-type and selenomethionine-labeled enzymes, hanging drop vapor diffusion method, 12 mg/ml protein from 50 mM Tris-HCl, pH 7.5, and 26% v/v PEG 550 MME supplemented with two detergents, n-heptyl-beta-D-thioglucopyranoside and CHAPSO, 16°C, X-ray diffraction structure determination and analysis at 2.59 and 3.60 A resolution, respectively

Escherichia coli

P23930

757766

using 8% (w/v) 2-methyl-2,4-pentanediol, 0.1 M MES pH 6.0 and 0.4 M ammonium citrate

Escherichia coli

P23930

748746

vapor diffusion method, using 50 mM ADA, pH 6.5, and 24% (w/v) PEG 400

Escherichia coli

-

749188

using 30% (w/v) PEG-500 DME, 0.1 M sodium citrate pH 5.0 and 0.1 M sodium acetate

Pseudomonas aeruginosa

Q9ZI86

748746