1.14.14.82: flavonoid 3'-monooxygenase

This is an abbreviated version!
For detailed information about flavonoid 3'-monooxygenase, go to the full flat file.

Reaction

Synonyms

CsF3'H, Cyp75B, CYP75B1, CYP75B3, CYP75B4, CYP75B50, EC 1.14.13.21, F3'H, flavonoid 3'-hydroxylase, flavonoid 3-hydroxylase, flavonoid 3�-hydroxylase, More, NADPH:flavonoid-3'-hydroxylase, oxygenase, flavonoid 3'-mono-, oxygenase, flavonoid 3-mono-, PR1, sb04g024710, SF3'H1

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor

                1.14.14.82 flavonoid 3'-monooxygenase

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Results	in table
4	Activating Compound
193	AA Sequence
4	Application
3	CAS Registry Number
46	Cloned(Commentary)
5	Cofactor
9	Expression
23	General Information
2	General Stability
30	Inhibitors
17	kcat/KM [mM/s]
47	KM Value [mM]
14	Localization
1	Metals/Ions
3	Molecular Weight [Da]
91	Natural Substrates/ Products (Substrates)
75	Organism
11	Pathway
27	pH Optimum
2	pH Range
2	pI Value
12	Protein Variants
1	Reaction
3	Reaction Type
75	Reference
97	Source Tissue
3	Specific Activity [micromol/min/mg]
3	Storage Stability
170	Substrates and Products (Substrate)
4	Subunits
100	Synonyms
1	Systematic Name
20	Temperature Optimum [°C]
1	Temperature Range [°C]
5	Temperature Stability [°C]
17	Turnover Number [1/s]

Storage Stability

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Storage Stability on EC 1.14.14.82 - flavonoid 3'-monooxygenase

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-70°C, storage of microsomes in presence of 14 mM mercaptoethanol and 15% sucrose, stable for several weeks

Petroselinum crispum

-

439004

-70°C, sucrose or glycerol plus dithiothreitol stabilize during purification and storage

Zea mays

-

439002

-80°C, microsomal preparations containing 10% sucrose, frozen in liquid nitrogen, stable

Sinningia cardinalis

-

439000