1.14.14.82: flavonoid 3'-monooxygenase

This is an abbreviated version!
For detailed information about flavonoid 3'-monooxygenase, go to the full flat file.

Reaction

Synonyms

CsF3'H, Cyp75B, CYP75B1, CYP75B3, CYP75B4, CYP75B50, EC 1.14.13.21, F3'H, flavonoid 3'-hydroxylase, flavonoid 3-hydroxylase, flavonoid 3�-hydroxylase, More, NADPH:flavonoid-3'-hydroxylase, oxygenase, flavonoid 3'-mono-, oxygenase, flavonoid 3-mono-, PR1, sb04g024710, SF3'H1

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor

                1.14.14.82 flavonoid 3'-monooxygenase

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Results	in table
4	Activating Compound
193	AA Sequence
4	Application
3	CAS Registry Number
46	Cloned(Commentary)
5	Cofactor
9	Expression
23	General Information
2	General Stability
30	Inhibitors
17	kcat/KM [mM/s]
47	KM Value [mM]
14	Localization
1	Metals/Ions
3	Molecular Weight [Da]
91	Natural Substrates/ Products (Substrates)
75	Organism
11	Pathway
27	pH Optimum
2	pH Range
2	pI Value
12	Protein Variants
1	Reaction
3	Reaction Type
75	Reference
97	Source Tissue
3	Specific Activity [micromol/min/mg]
3	Storage Stability
170	Substrates and Products (Substrate)
4	Subunits
100	Synonyms
1	Systematic Name
20	Temperature Optimum [°C]
1	Temperature Range [°C]
5	Temperature Stability [°C]
17	Turnover Number [1/s]

KM Value

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KM Value on EC 1.14.14.82 - flavonoid 3'-monooxygenase

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KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.0074 - 0.0079

5-deoxyleucopelargonidin

2 entries

0.000072 - 0.048

apigenin

10 entries

0.00103 - 0.1436

dihydrokaempferol

10 entries

0.000098 - 0.06806

kaempferol

9 entries

0.000108 - 24

naringenin

16 entries

0.0074

5-deoxyleucopelargonidin

Arabidopsis thaliana

Q9SD85

pH 7.3, 25°C

746151

0.0079

5-deoxyleucopelargonidin

Tagetes erecta

C1J0N9

pH 7.0, 25°C

746151

0.000072

apigenin

Oryza sativa Japonica Group

Q7G602, Q8LM92

pH 7.5, 30°C, enzyme from white rice

742706

0.000085

apigenin

Oryza sativa Japonica Group

Q7G602, Q8LM92

pH 7.5, 30°C, enzyme from black rice

742706

0.0001

apigenin

Fragaria x ananassa

T1NXM4

pH 7.0, 25°C

726210

0.00024

apigenin

Oryza sativa Japonica Group

Q7G602, Q8LM92

pH 7.5, 30°C, enzyme from white rice

742706

0.0054

apigenin

Tagetes erecta

C1J0N9

pH 7.0, 25°C

746151

0.0058

apigenin

Fragaria x ananassa

-

pH 7.0, 25°C

746151

0.0064

apigenin

Arabidopsis thaliana

Q9SD85

pH 7.3, 25°C

746151

0.019

apigenin

Perilla frutescens

Q93XJ2

-

439008

0.021

apigenin

Torenia sp.

-

-

439009

0.048

apigenin

Fragaria vesca

T1NXM1

pH 7.0, 25°C

726210

0.00103

dihydrokaempferol

Oryza sativa Japonica Group

Q7G602, Q8LM92

pH 7.5, 30°C, enzyme from black rice

742706

0.0013

dihydrokaempferol

Dahlia pinnata

D3IWE5; D3IWE6

allele 2, pH 7.5, 30°C

744156

0.002

dihydrokaempferol

Tagetes erecta

C1J0N9

pH 7.0, 25°C

746151

0.002494

dihydrokaempferol

Oryza sativa Japonica Group

Q7G602, Q8LM92

pH 7.5, 30°C, enzyme from white rice

742706

0.0035

dihydrokaempferol

Dahlia pinnata

D3IWE5; D3IWE6

allele 1, pH 7.5, 30°C

744156

0.004

dihydrokaempferol

Torenia sp.

-

-

439009

0.009

dihydrokaempferol

Fragaria x ananassa

-

pH 7.0, 25°C

746151

0.009

dihydrokaempferol

Arabidopsis thaliana

Q9SD85

pH 7.3, 25°C

746151

0.02

dihydrokaempferol

Perilla frutescens

Q93XJ2

-

439008

0.1436

dihydrokaempferol

Camellia sinensis

A0A0S2LLA9

pH 7.0, 30°C

742707

0.000098

kaempferol

Oryza sativa Japonica Group

Q7G602, Q8LM92

pH 7.5, 30°C, enzyme from black rice

742706

0.00011

kaempferol

Oryza sativa Japonica Group

Q7G602, Q8LM92

pH 7.5, 30°C, enzyme from white rice

742706

0.001

kaempferol

Fragaria x ananassa

-

pH 7.0, 25°C

746151

0.0013

kaempferol

Tagetes erecta

C1J0N9

pH 7.0, 25°C

746151

0.0019

kaempferol

Arabidopsis thaliana

Q9SD85

pH 7.3, 25°C

746151

0.0029

kaempferol

Oryza sativa Japonica Group

Q7G602, Q8LM92

pH 7.5, 30°C, enzyme from white rice

742706

0.003

kaempferol

Torenia sp.

-

-

439009

0.00715

kaempferol

Columnea hybrida

-

-

438999

0.06806

kaempferol

Camellia sinensis

A0A0S2LLA9

pH 7.0, 30°C

742707

0.000108

naringenin

Oryza sativa Japonica Group

Q7G602, Q8LM92

pH 7.5, 30°C, enzyme from black rice

742706

0.000286

naringenin

Oryza sativa Japonica Group

Q7G602, Q8LM92

pH 7.5, 30°C, enzyme from white rice

742706

0.0005

naringenin

Dahlia pinnata

D3IWE5; D3IWE6

allele 2, pH 7.5, 30°C

744156

0.0008

naringenin

Torenia sp.

-

-

439009

0.0009

naringenin

Dahlia pinnata

D3IWE5; D3IWE6

allele 1, pH 7.5, 30°C

744156

0.001

naringenin

Fragaria x ananassa

-

pH 7.0, 25°C

746151

0.001

naringenin

Fragaria x ananassa

T1NXM4

pH 7.0, 25°C

726210

0.0012

naringenin

Arabidopsis thaliana

Q9SD85

pH 7.3, 25°C

746151

0.003

naringenin

Tagetes erecta

C1J0N9

pH 7.0, 25°C

746151

0.004

naringenin

Fragaria vesca

T1NXM1

pH 7.0, 25°C

726210

0.009

naringenin

Fragaria x ananassa

T1NXM4

pH 7.0, 25°C

726210

0.0171

naringenin

Camellia sinensis

A0A0S2LLA9

pH 7.0, 30°C

742707

0.02

naringenin

Perilla frutescens

Q93XJ2

-

439008

0.021

naringenin

Fragaria vesca

T1NXM1

pH 7.0, 25°C

726210

0.022

naringenin

Fragaria vesca

T1NXM1

pH 7.0, 25°C

726210

24

naringenin

Citrus sinensis

-

-

439010