1.14.14.82: flavonoid 3'-monooxygenase
This is an abbreviated version!
For detailed information about flavonoid 3'-monooxygenase, go to the full flat file.
Word Map on EC 1.14.14.82
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1.14.14.82
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anthocyanins
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naringenin
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flavanones
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flavonols
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kaempferol
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dihydrokaempferol
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dihydroflavonols
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b-ring
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delphinidin
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3',5'-hydroxylase
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5'-hydroxylase
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4-reductase
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f3\'hs
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pubescence
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dihydromyricetin
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nutrition
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synthesis
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agriculture
- 1.14.14.82
- anthocyanins
- naringenin
- flavanones
- flavonols
- kaempferol
- dihydrokaempferol
- dihydroflavonols
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b-ring
- delphinidin
-
3',5'-hydroxylase
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5'-hydroxylase
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4-reductase
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f3\'hs
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pubescence
- dihydromyricetin
- nutrition
- synthesis
- agriculture
Reaction
Synonyms
CsF3'H, Cyp75B, CYP75B1, CYP75B3, CYP75B4, CYP75B50, EC 1.14.13.21, F3'H, flavonoid 3'-hydroxylase, flavonoid 3-hydroxylase, flavonoid 3’-hydroxylase, More, NADPH:flavonoid-3'-hydroxylase, oxygenase, flavonoid 3'-mono-, oxygenase, flavonoid 3-mono-, PR1, sb04g024710, SF3'H1
ECTree
1.14.14.82 flavonoid 3'-monooxygenaseAdvanced search results
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results (Engineering
Engineering on EC 1.14.14.82 - flavonoid 3'-monooxygenase
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PROTEIN VARIANTS 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
V425A
D3IWE5; D3IWE6
mutation in allele 2, complete loss of the ability th hydroxylate the 6'-deoxychalcone isoliquiritigenin in position 3, i.e. reaction of chalcone 3-hydroxylase
T484F
site-directed mutagenesis, altered substrate specificity compared to the wild-type enzyme
T487A
site-directed mutagenesis, altered substrate specificity compared to the wild-type enzyme
T487S
site-directed mutagenesis, a conservative Thr to Ser exchange at position 487 conferred additional 5'-hydroxylation activity to recombinant Gerbera hybrida F3'H
Y484F/T487S
site-directed mutagenesis, altered substrate specificity compared to the wild-type enzyme
additional information
additional information
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recombinant expression of the flavonoid 3',5'-hydroxylase, EC 1.14.13.88, from Petunia sp. in Dendrathemum grandiflora plants leads to competition of the endogenous flavonoid 3'-hydroxylase with the recombinant enzyme for the same substrates in the flavonoid biosynthetic pathway leading to a change in flower petal colors, phenotype, overview
additional information
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loss of enzyme activity leads to accumulation of 3,5-di-O-(beta-glucopyranosyl)pelargonidin 6''-O-4,6'''-O-1-cyclic malate instead of 3,5-di-O-(beta-glucopyranosyl)cyanidin 6''-O-4,6'''-O-1-cyclic malate, the only difference between these two anthocyanins is a hydroxyl grouppresent at the 3' position in the B-ring of aglycone. Loss of enzme results in a color change in buds from purple to deep pink. Mutation is due to an active hAT type transposable element, designated Tdic101. The color change is attributed to Tdic101 insertion into the second intron of flavonoid 3'-hydroxylase
additional information
generation of chimeric constructs consisting of cDNA fragments of Gerbera hybrida F3'H and Osteospermum hybrida F3'5'H, EC 1.14.13.88, overview
additional information
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downregulation of the F3'H gene expression by virus-induced gene silencing in soybean does not alter the tawny pubescence pigmentation proved to be unchanged in greenhouse-grown plants, but is reduced when the steady-state mRNA level of the F3'H gene is reduced to approximately 5% of that of greenhouse-grown plants, phenotypes, overview
additional information
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an enzyme-deficient mutant line shows altered flower color, i.e. magenta flowers instead of bright blue due to accumulation of dihydrokaempherol instead of dihydroquercetin
additional information
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an enzyme-deficient mutant line shows altered flower color, i.e. pink flowers instead of dark purple due to accumulation of dihydrokaempherol instead of dihydroquercetin
additional information
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an enzyme-deficient mutant line shows altered flower color, i.e. fuchsia flowers instead of bright blue due to accumulation of dihydrokaempherol instead of dihydroquercetin
