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1.14.14.82: flavonoid 3'-monooxygenase

This is an abbreviated version!
For detailed information about flavonoid 3'-monooxygenase, go to the full flat file.

Word Map on EC 1.14.14.82

Reaction

a flavonoid
+
[reduced NADPH-hemoprotein reductase]
 +
O2
=
a 3'-hydroxyflavonoid
 +
[oxidized NADPH-hemoprotein reductase]
 +
H2O

Synonyms

CsF3'H, Cyp75B, CYP75B1, CYP75B3, CYP75B4, CYP75B50, EC 1.14.13.21, F3'H, flavonoid 3'-hydroxylase, flavonoid 3-hydroxylase, flavonoid 3’-hydroxylase, More, NADPH:flavonoid-3'-hydroxylase, oxygenase, flavonoid 3'-mono-, oxygenase, flavonoid 3-mono-, PR1, sb04g024710, SF3'H1

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.14.82 flavonoid 3'-monooxygenase

Cloned

Cloned on EC 1.14.14.82 - flavonoid 3'-monooxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, detailed phylogenetic analysis and comparison of sequences of both enzymes indicate that F3',5'H is recruited from F3'H before the divergence of angiosperms and gymnosperms, overview
DNA and amino acid sequence determination and analysis, genetic mapping, phylogenetic analysis, expression analysis and metabolic profiling, phenotypes
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expression in Escherichia coli JM109
expression in Petunia hybrida
expression in Saccharomyces cerevisiae
expression in Saccharomyces cerevisiae WAT11
expression in yeast strain WAT11
expression of wild-type enzyme and chimeric mutants in yeast strain INVSc 1 microsomes
gene F3'H, DNA and amino acid sequence determination and analysis of wild-type and mutant genes, genomic organization, overview
gene F3'H, DNA and amino acid sequence determination and analysis, phylogenetic tree, developmental expression analysis, comparison of red and white cultivar enzyme expression levels, functional ectopic expression in Petunia hybrida altering flower color and flavonoid composition, overview
gene F3'H-1, two clones, DNA and amio acid sequence determination and analysis, sequence comparison, expression analysis, alternative polyadenylation in the 3'-UTR is adopted by this gene to generate heterogeneous transcripts, phylogenetic tree, functional expression of His-tagged F3'H-1 in Escherichia coli
gene F3'h1, DNA and amino acid sequence determination and analysis, genomic structure, expression pattern analysis
gene F3'H1, DNA and amio acid sequence determination and analysis, phylogenetic tree
gene F3'H1, DNA and amio acid sequence determination and analysis, phylogenetic tree, overexpression of F3'H1 under control of the CaMV 35S promoter using the Agrobacterium tumefaciens transfection method
gene F3'h1, DNA, amino acid sequence, and promoter determination and analysis, the promoter region of the gene contains a putative G-box, two MYB-binding domains, and TA-repeats, the structure and number of the TA repeats is cultivar-dependent and highly polymorphic, the enzyme is encoded in the Tau locus involved in control of the pubescens and seed coat color, genetic structure and organization, dominant Tau and recessive tau allele of the locus produce tawny and gray pubescence, respectively, alleles at the locus are associated with chilling tolerance, overview
gene F3'h2, DNA and amino acid sequence determination and analysis, genomic structure, expression pattern analysis
gene F3'H2, DNA and amino acid sequence determination and analysis, phylogenetic tree, overexpression of F3'H1 under control of the CaMV 35S promoter using the Agrobacterium tumefaciens transfection method
gene F3'h3, DNA and amino acid sequence determination and analysis, genomic structure, expression pattern analysis
gene F3'h4, DNA and amino acid sequence determination and analysis, genomic structure, expression pattern analysis
gene GmF3'H, DNA and amino acid sequence determination and analysis of 19 different cultivars, linked to the pleiotropic T locus and 2 recessive alleles, determination of the tissue expression pattern of gene GmF3'H
-
gene MdF3'HI, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, functional ectopic expression in Arabidopsis thaliana tt7 mutant and in Nicotiana tabacum
gene MdF3'HIIa, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, functional ectopic expression in Arabidopsis thaliana tt7 mutant and in Nicotiana tabacum
gene MdF3'HIIb, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, functional ectopic expression in Arabidopsis thaliana tt7 mutant and in Nicotiana tabacum
overexpression in tobacco plants leads to the accumulation of flavonoids and to an increase of flower colour intensity
predominantly expressed in red form of plant
sf3'h1 gene, the enzyme is encoded at the T locus, quantitative expression analysis in wild-type and mutant plants
-
two genes F3'H, DNA and amino acid sequence determination, genomic analysis and expression analysis in strain PN40024, phylogenetic tree, overview
-