1.14.14.176: taxadiene 5alpha-hydroxylase
This is an abbreviated version!
For detailed information about taxadiene 5alpha-hydroxylase, go to the full flat file.
Word Map on EC 1.14.14.176
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1.14.14.176
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taxol
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paclitaxel
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medicine
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taxane
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synthesis
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bottleneck
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oxygenases
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nanodiscs
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drug development
- 1.14.14.176
- taxol
- paclitaxel
- medicine
-
taxane
- synthesis
-
bottleneck
- oxygenases
-
nanodiscs
- drug development
Reaction
Synonyms
5alphaCYP, CYP725A4, EC 1.14.99.37, oxygenase, taxadiene 5-mono-, taxa-4(5),11(12)-diene 5-hydroxylase, taxa-4(5),11(12)-diene 5alpha-hydroxylase, taxadien-5alpha-hydroxylase, taxadiene 5-hydroxylase, taxadiene 5-monooxygenase, taxadiene synthase, taxadiene-5alpha-hydroxylase
ECTree
1.14.14.176 taxadiene 5alpha-hydroxylaseAdvanced search results
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results (Cloned
Cloned on EC 1.14.14.176 - taxadiene 5alpha-hydroxylase
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CLONED (Commentary) 
ORGANISM
UNIPROT
LITERATURE
cDNAs encoding taxa-4(5),11(12)-diene synthase and CYP725A4 are introduced in Nicotiana sylvestris for specific expression in trichome cells. Production of truncated TS in Escherichia coli. The TS cDNA coding for a protein truncated at the N-terminus at amino acid position 75 is cloned in the pET-30b expression vector. Furthermore, CYP725A4 is expressed in yeast.
CYP725A4, DNA and amino acid sequence determination and analysis, expression in the Saccharomyces cerevisiae WAT11 strain containing the Arabidopsis reductase ATR1, and co-expression in Nicotiana sylvestris with taxa-4(5),11(12)-diene synthase, which does not lead to 5alpha-hydroxytaxa-4(20),11(12)-diene, but to formation of 5(12)-oxa-3(11)-cyclotaxane, a tritiated oxidized taxane, structural analysis of the compound, phenotype, overview
