2.3.1.254: N-terminal methionine Nalpha-acetyltransferase NatB
This is an abbreviated version!
For detailed information about N-terminal methionine Nalpha-acetyltransferase NatB, go to the full flat file.
Word Map on EC 2.3.1.254
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2.3.1.254
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auxiliary
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tropomyosins
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cable
-
nalpha-terminal
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unacetylated
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met-asp
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anti-fcepsilonri
- 2.3.1.254
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auxiliary
- tropomyosins
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cable
-
nalpha-terminal
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unacetylated
- met-asp
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anti-fcepsilonri
Reaction
Synonyms
CRA-1, EC 2.3.1.88, MDM20, Mdm20p, N-acetyltransferase 3, N-terminal acetyltransferase, N-terminal acetyltransferase B complex catalytic subunit NAA20, NAA20, NAA25, Naa50p, NAT3, Nat3p, NAT5/SAN, natB, NatB N-alpha-terminal acetylation complex, TCU2
ECTree
2.3.1.254 N-terminal methionine Nalpha-acetyltransferase NatBAdvanced search results
results (
results (Substrates Products
Substrates Products on EC 2.3.1.254 - N-terminal methionine Nalpha-acetyltransferase NatB
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + an N-terminal L-methionyl-L-asparaginyl-[protein]
an N-terminal Nalpha-acetyl-L-methionyl-L-asparginyl-[protein] + CoA
acetyl-CoA + an N-terminal L-methionyl-L-aspartyl-[protein]
an N-terminal Nalpha-acetyl-L-methionyl-L-aspartyl-[protein] + CoA
acetyl-CoA + an N-terminal L-methionyl-L-glutaminyl-[protein]
an N-terminal Nalpha-acetyl-L-methionyl-L-glutaminyl-[protein] + CoA
acetyl-CoA + an N-terminal L-methionyl-L-glutamyl-[protein]
an N-terminal Nalpha-acetyl-L-methionyl-L-glutamyl-[protein] + CoA
acetyl-CoA + MFGPEEGGRWGRPVGRRRRRPVRVYP
CoA + N-acetyl-MFGPEEGGRWGRPVGRRRRRPVRVYP
-
-
-
-
?
acetyl-CoA + MIGPEEGGRWGRPVGRRRRRPVRVYP
CoA + N-acetyl-MIGPEEGGRWGRPVGRRRRRPVRVYP
-
-
-
-
?
acetyl-CoA + MLALISRRWGRPVGRRRRRPVRVYP
CoA + N-acetyl-MLALISRRWGRPVGRRRRRPVRVYP
-
-
-
-
?
acetyl-CoA + MLDPEEGGRWGRPVGRRRRRPVRVYP
CoA + N-acetyl-MLDPEEGGRWGRPVGRRRRRPVRVYP
-
-
-
-
?
acetyl-CoA + MLGPEGGRWGRPVGRRRRRPVRVYP
CoA + N-acetyl-MLGPEGGRWGRPVGRRRRRPVRVYP
-
-
-
-
?
acetyl-CoA + MLGTEEGGRWGRPVGRRRRRPVRVYP
CoA + N-acetyl-MLGTEEGGRWGRPVGRRRRRPVRVYP
-
-
-
-
?
acetyl-CoA + MLGTGPARWGRPVGRRRRRPVRVYP
CoA + N-acetyl-MLGTGPARWGRPVGRRRRRPVRVYP
-
-
-
-
?
acetyl-CoA + MLLPEEGGRWGRPVGRRRRRPVRVYP
CoA + N-acetyl- MLLPEEGGRWGRPVGRRRRRPVRVYP
-
-
-
-
?
acetyl-CoA + MLRPEEGGRWGRPVGRRRRRPVRVYP
CoA + N-acetyl-MLRPEEGGRWGRPVGRRRRRPVRVYP
-
-
-
-
?
acetyl-CoA + N-terminal L-methionyl-L-aspartyl-[Bax]
an N-terminal Nalpha-acetyl-L-methionyl-L-aspartyl-[Bax] + CoA
acetyl-CoA + N-terminal L-methionyl-L-glutaminyl-[influenza virus PA-X]
an N-terminal Nalpha-acetyl-L-methionyl-L-glutaminyl-[influenza virus PA-X] + CoA
acetyl-CoA + N-terminal L-methionyl-L-glutaminyl-[influenza virus PA-X]
N-terminal Nalpha-acetyl-L-methionyl-L-glutaminyl-[influenza virus PA-X] + CoA
acetyl-CoA + an N-terminal L-methionyl-L-asparaginyl-[protein]
an N-terminal Nalpha-acetyl-L-methionyl-L-asparginyl-[protein] + CoA
Q5AAR6; Q5AB99
-
-
-
?
acetyl-CoA + an N-terminal L-methionyl-L-asparaginyl-[protein]
an N-terminal Nalpha-acetyl-L-methionyl-L-asparginyl-[protein] + CoA
Q5AAR6; Q5AB99
-
-
-
?
acetyl-CoA + an N-terminal L-methionyl-L-asparaginyl-[protein]
an N-terminal Nalpha-acetyl-L-methionyl-L-asparginyl-[protein] + CoA
P61599; Q14CX7
-
-
-
?
acetyl-CoA + an N-terminal L-methionyl-L-asparaginyl-[protein]
an N-terminal Nalpha-acetyl-L-methionyl-L-asparginyl-[protein] + CoA
P61600; Q8BWZ3
-
-
-
?
acetyl-CoA + an N-terminal L-methionyl-L-asparaginyl-[protein]
an N-terminal Nalpha-acetyl-L-methionyl-L-asparginyl-[protein] + CoA
P61600; Q8BWZ3
-
-
-
?
acetyl-CoA + an N-terminal L-methionyl-L-asparaginyl-[protein]
an N-terminal Nalpha-acetyl-L-methionyl-L-asparginyl-[protein] + CoA
Q06504; Q12387
-
-
-
?
acetyl-CoA + an N-terminal L-methionyl-L-asparaginyl-[protein]
an N-terminal Nalpha-acetyl-L-methionyl-L-asparginyl-[protein] + CoA
-
-
-
-
?
acetyl-CoA + an N-terminal L-methionyl-L-asparaginyl-[protein]
an N-terminal Nalpha-acetyl-L-methionyl-L-asparginyl-[protein] + CoA
Q06504; Q12387
-
-
-
?
acetyl-CoA + an N-terminal L-methionyl-L-aspartyl-[protein]
an N-terminal Nalpha-acetyl-L-methionyl-L-aspartyl-[protein] + CoA
Q5AAR6; Q5AB99
-
-
-
?
acetyl-CoA + an N-terminal L-methionyl-L-aspartyl-[protein]
an N-terminal Nalpha-acetyl-L-methionyl-L-aspartyl-[protein] + CoA
Q5AAR6; Q5AB99
-
-
-
?
acetyl-CoA + an N-terminal L-methionyl-L-aspartyl-[protein]
an N-terminal Nalpha-acetyl-L-methionyl-L-aspartyl-[protein] + CoA
P61599; Q14CX7
-
-
-
?
acetyl-CoA + an N-terminal L-methionyl-L-aspartyl-[protein]
an N-terminal Nalpha-acetyl-L-methionyl-L-aspartyl-[protein] + CoA
P61600; Q8BWZ3
-
-
-
?
acetyl-CoA + an N-terminal L-methionyl-L-aspartyl-[protein]
an N-terminal Nalpha-acetyl-L-methionyl-L-aspartyl-[protein] + CoA
P61600; Q8BWZ3
-
-
-
?
acetyl-CoA + an N-terminal L-methionyl-L-aspartyl-[protein]
an N-terminal Nalpha-acetyl-L-methionyl-L-aspartyl-[protein] + CoA
Q06504; Q12387
-
-
-
?
acetyl-CoA + an N-terminal L-methionyl-L-aspartyl-[protein]
an N-terminal Nalpha-acetyl-L-methionyl-L-aspartyl-[protein] + CoA
-
-
-
-
?
acetyl-CoA + an N-terminal L-methionyl-L-aspartyl-[protein]
an N-terminal Nalpha-acetyl-L-methionyl-L-aspartyl-[protein] + CoA
Q06504; Q12387
-
-
-
?
acetyl-CoA + an N-terminal L-methionyl-L-glutaminyl-[protein]
an N-terminal Nalpha-acetyl-L-methionyl-L-glutaminyl-[protein] + CoA
Q5AAR6; Q5AB99
-
-
-
?
acetyl-CoA + an N-terminal L-methionyl-L-glutaminyl-[protein]
an N-terminal Nalpha-acetyl-L-methionyl-L-glutaminyl-[protein] + CoA
Q5AAR6; Q5AB99
-
-
-
?
acetyl-CoA + an N-terminal L-methionyl-L-glutaminyl-[protein]
an N-terminal Nalpha-acetyl-L-methionyl-L-glutaminyl-[protein] + CoA
P61600; Q8BWZ3
-
-
-
?
acetyl-CoA + an N-terminal L-methionyl-L-glutaminyl-[protein]
an N-terminal Nalpha-acetyl-L-methionyl-L-glutaminyl-[protein] + CoA
P61600; Q8BWZ3
-
-
-
?
acetyl-CoA + an N-terminal L-methionyl-L-glutaminyl-[protein]
an N-terminal Nalpha-acetyl-L-methionyl-L-glutaminyl-[protein] + CoA
Q06504; Q12387
-
-
-
?
acetyl-CoA + an N-terminal L-methionyl-L-glutaminyl-[protein]
an N-terminal Nalpha-acetyl-L-methionyl-L-glutaminyl-[protein] + CoA
Q06504; Q12387
-
-
-
?
acetyl-CoA + an N-terminal L-methionyl-L-glutamyl-[protein]
an N-terminal Nalpha-acetyl-L-methionyl-L-glutamyl-[protein] + CoA
Q5AAR6; Q5AB99
-
-
-
?
acetyl-CoA + an N-terminal L-methionyl-L-glutamyl-[protein]
an N-terminal Nalpha-acetyl-L-methionyl-L-glutamyl-[protein] + CoA
Q5AAR6; Q5AB99
-
-
-
?
acetyl-CoA + an N-terminal L-methionyl-L-glutamyl-[protein]
an N-terminal Nalpha-acetyl-L-methionyl-L-glutamyl-[protein] + CoA
P61599; Q14CX7
-
-
-
?
acetyl-CoA + an N-terminal L-methionyl-L-glutamyl-[protein]
an N-terminal Nalpha-acetyl-L-methionyl-L-glutamyl-[protein] + CoA
P61600; Q8BWZ3
-
-
-
?
acetyl-CoA + an N-terminal L-methionyl-L-glutamyl-[protein]
an N-terminal Nalpha-acetyl-L-methionyl-L-glutamyl-[protein] + CoA
P61600; Q8BWZ3
-
-
-
?
acetyl-CoA + an N-terminal L-methionyl-L-glutamyl-[protein]
an N-terminal Nalpha-acetyl-L-methionyl-L-glutamyl-[protein] + CoA
Q06504; Q12387
-
-
-
?
acetyl-CoA + an N-terminal L-methionyl-L-glutamyl-[protein]
an N-terminal Nalpha-acetyl-L-methionyl-L-glutamyl-[protein] + CoA
-
-
-
-
?
acetyl-CoA + an N-terminal L-methionyl-L-glutamyl-[protein]
an N-terminal Nalpha-acetyl-L-methionyl-L-glutamyl-[protein] + CoA
Q06504; Q12387
-
-
-
?
acetyl-CoA + N-terminal L-methionyl-L-aspartyl-[Bax]
an N-terminal Nalpha-acetyl-L-methionyl-L-aspartyl-[Bax] + CoA
P61600; Q8BWZ3
heterologous expression of human Bax in mouse embryonic fibroblasts (MEFs). Recombinant human Bax is N-terminally (Nt-)acetylated by Naa20 and that Nt-acetylation of Bax is essential to maintain Bax in an inactive conformation in the cytosol of MEF cells. Human Bax displays the N-terminal Met-Asp amino-acid sequence
-
-
?
acetyl-CoA + N-terminal L-methionyl-L-aspartyl-[Bax]
an N-terminal Nalpha-acetyl-L-methionyl-L-aspartyl-[Bax] + CoA
P61600; Q8BWZ3
heterologous expression of human Bax in mouse embryonic fibroblasts (MEFs). Recombinant human Bax is N-terminally (Nt-)acetylated by Naa20 and that Nt-acetylation of Bax is essential to maintain Bax in an inactive conformation in the cytosol of MEF cells. Human Bax displays the N-terminal Met-Asp amino-acid sequence
-
-
?
acetyl-CoA + N-terminal L-methionyl-L-aspartyl-[Bax]
an N-terminal Nalpha-acetyl-L-methionyl-L-aspartyl-[Bax] + CoA
Q06504; Q12387
heterologous expression of human Bax in Saccharomyces cerevisiae. Recombinant human Bax is N-terminal (Nt-)acetylated by yNaa20p and that Nt-acetylation of Bax is essential to maintain Bax in an inactive conformation in the cytosol of yeast. Human Bax displays the N-terminal Met-Asp amino-acid sequence
-
-
?
acetyl-CoA + N-terminal L-methionyl-L-aspartyl-[Bax]
an N-terminal Nalpha-acetyl-L-methionyl-L-aspartyl-[Bax] + CoA
Q06504; Q12387
heterologous expression of human Bax in Saccharomyces cerevisiae. Recombinant human Bax is N-terminal (Nt-)acetylated by yNaa20p and that Nt-acetylation of Bax is essential to maintain Bax in an inactive conformation in the cytosol of yeast. Human Bax displays the N-terminal Met-Asp amino-acid sequence
-
-
?
acetyl-CoA + N-terminal L-methionyl-L-glutaminyl-[influenza virus PA-X]
an N-terminal Nalpha-acetyl-L-methionyl-L-glutaminyl-[influenza virus PA-X] + CoA
Q06504; Q12387
-
-
-
?
acetyl-CoA + N-terminal L-methionyl-L-glutaminyl-[influenza virus PA-X]
an N-terminal Nalpha-acetyl-L-methionyl-L-glutaminyl-[influenza virus PA-X] + CoA
Q06504; Q12387
-
-
-
?
acetyl-CoA + N-terminal L-methionyl-L-glutaminyl-[influenza virus PA-X]
N-terminal Nalpha-acetyl-L-methionyl-L-glutaminyl-[influenza virus PA-X] + CoA
P61599; Q14CX7
-
-
-
?
acetyl-CoA + N-terminal L-methionyl-L-glutaminyl-[influenza virus PA-X]
N-terminal Nalpha-acetyl-L-methionyl-L-glutaminyl-[influenza virus PA-X] + CoA
P61599; Q14CX7
the enzyme NatB acetylates the N-terminal amino acid of polymerase acidic proteins, especially PA-X, of influenza A virus. PA-X starts with Met-Glu. Preparation of a series of plasmids encoding PA-X mutants, which possess a NatB-permissive substitution at the second amino acid of E to D (PA-X E2D), to N (PA-X E2N), or to A (PA-X E2A), which is recognized by NatA, but not NatB, or to P (PA-X E2P)
-
-
?
additional information
?
-
Q5AAR6; Q5AB99
substrate specificity of NatB is determined by the first two amino acids of the substrate protein/peptide. The substrate's N-terminus is anchored into the NatB catalytic pocket by hydrogen bonds. The first two amino acids Met and Asp of a substrate peptide mediate the major interactions with the active site in the Naa20 subunit. The hydrogen bonds between the substrate Asp and pocket residues of Naa20 are essential to determine the NatB substrate specificity. A hydrogen bond between the amino group of the substrate Met and a carbonyl group in the Naa20 active site directly anchors the substrate toward acetyl-CoA. NatB has a unique substrate specificity different from all other NATs, which requires acidic amino acids or their amides at the second position. No activity with the NatC substrate MLRFVTANSQDNGRPVGRK and with the NatA substrate SASEAG
-
-
-
additional information
?
-
-
substrate specificity of NatB is determined by the first two amino acids of the substrate protein/peptide. The substrate's N-terminus is anchored into the NatB catalytic pocket by hydrogen bonds. The first two amino acids Met and Asp of a substrate peptide mediate the major interactions with the active site in the Naa20 subunit. The hydrogen bonds between the substrate Asp and pocket residues of Naa20 are essential to determine the NatB substrate specificity. A hydrogen bond between the amino group of the substrate Met and a carbonyl group in the Naa20 active site directly anchors the substrate toward acetyl-CoA. NatB has a unique substrate specificity different from all other NATs, which requires acidic amino acids or their amides at the second position. No activity with the NatC substrate MLRFVTANSQDNGRPVGRK and with the NatA substrate SASEAG
-
-
-
additional information
?
-
Q5AAR6; Q5AB99
substrate specificity of NatB is determined by the first two amino acids of the substrate protein/peptide. The substrate's N-terminus is anchored into the NatB catalytic pocket by hydrogen bonds. The first two amino acids Met and Asp of a substrate peptide mediate the major interactions with the active site in the Naa20 subunit. The hydrogen bonds between the substrate Asp and pocket residues of Naa20 are essential to determine the NatB substrate specificity. A hydrogen bond between the amino group of the substrate Met and a carbonyl group in the Naa20 active site directly anchors the substrate toward acetyl-CoA. NatB has a unique substrate specificity different from all other NATs, which requires acidic amino acids or their amides at the second position. No activity with the NatC substrate MLRFVTANSQDNGRPVGRK and with the NatA substrate SASEAG
-
-
-
additional information
?
-
-
no activity with MKEEVKGRWGRPVGRRRRRPVRVYP and MDELFPLRWGRPVGRRRRRPVRVYP
-
-
?
additional information
?
-
-
the enzyme also shows lysine Nepsilon-acetyltransferase activity with histone 4
-
-
?
additional information
?
-
-
the enzyme preferably acetylates oligopeptides with N-termini Met-Leu-Xxx-Pro. Furthermore, the enzyme autoacetylates lysines 34, 37, and 140 in vitro
-
-
?
additional information
?
-
P61599; Q14CX7
the enzyme NatB acetylates the N-terminal amino acid of polymerase acidic proteins, especially PA-X, of influenza A virus. NatB prefers to acetylate proteins beginning with Met-Asp, Met-Glu, and Met-Asn in yeast and mammals
-
-
-
additional information
?
-
Q06504; Q12387
NatB catalyzes the N-terminal acetylation of the N-terminal amino acid of its target proteins and prefers to acetylate proteins beginning with Met-Asp, Met-Glu, and Met-Asn
-
-
-
additional information
?
-
Q06504; Q12387
NatB catalyzes the N-terminal acetylation of the N-terminal amino acid of its target proteins and prefers to acetylate proteins beginning with Met-Asp, Met-Glu, and Met-Asn
-
-
-
