2.3.1.254: N-terminal methionine Nalpha-acetyltransferase NatB

This is an abbreviated version!
For detailed information about N-terminal methionine Nalpha-acetyltransferase NatB, go to the full flat file.

Reaction

Synonyms

CRA-1, EC 2.3.1.88, MDM20, Mdm20p, N-acetyltransferase 3, N-terminal acetyltransferase, N-terminal acetyltransferase B complex catalytic subunit NAA20, NAA20, NAA25, Naa50p, NAT3, Nat3p, NAT5/SAN, natB, NatB N-alpha-terminal acetylation complex, TCU2

ECTree

     2 Transferases

         2.3 Acyltransferases

             2.3.1 Transferring groups other than aminoacyl groups

                2.3.1.254 N-terminal methionine Nalpha-acetyltransferase NatB

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Results	in table
49	AA Sequence
2	Cloned(Commentary)
5	Cofactor
1	Crystallization (Commentary)
23	Disease/ Diagnostics
35	General Information
1	Inhibitors
10	KM Value [mM]
5	Localization
35	Natural Substrates/ Products (Substrates)
86	Organism
2	Pathway
2	PDB ID
1	pH Optimum
36	Protein Variants
2	Purification (Commentary)
4	Reaction
15	Reference
6	Source Tissue
58	Substrates and Products (Substrate)
9	Subunits
55	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
6	Turnover Number [1/s]

Engineering

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Engineering on EC 2.3.1.254 - N-terminal methionine Nalpha-acetyltransferase NatB

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

A77S

Candida albicans

Q5AAR6; Q5AB99

site-directed mutagenesis of the Naa20 subunit, altered reaction kinetics compared to wild-type enzyme

758486

E25A

Candida albicans

Q5AAR6; Q5AB99

site-directed mutagenesis of the Naa20 subunit, altered reaction kinetics compared to wild-type enzyme

758486

F112A

Candida albicans

Q5AAR6; Q5AB99

site-directed mutagenesis of the Naa20 subunit, altered reaction kinetics compared to wild-type enzyme

758486

F112H

Candida albicans

Q5AAR6; Q5AB99

site-directed mutagenesis of the Naa20 subunit, altered reaction kinetics compared to wild-type enzyme

758486

F27A

Candida albicans

Q5AAR6; Q5AB99

site-directed mutagenesis of the Naa20 subunit, altered reaction kinetics compared to wild-type enzyme

758486

F27Y

Candida albicans

Q5AAR6; Q5AB99

site-directed mutagenesis of the Naa20 subunit, altered reaction kinetics compared to wild-type enzyme

758486

F490A/F493A

Candida albicans

Q5AAR6; Q5AB99

site-directed mutagenesis of the Naa25 subunit

758486

G140A

Candida albicans

Q5AAR6; Q5AB99

site-directed mutagenesis of the Naa20 subunit, altered reaction kinetics compared to wild-type enzyme

758486

H74A

Candida albicans

Q5AAR6; Q5AB99

site-directed mutagenesis of the Naa20 subunit, altered reaction kinetics compared to wild-type enzyme

758486

H74A/T76A

Candida albicans

Q5AAR6; Q5AB99

site-directed mutagenesis of the Naa20 subunit, altered reaction kinetics compared to wild-type enzyme

758486

L23A

Candida albicans

Q5AAR6; Q5AB99

site-directed mutagenesis of the Naa20 subunit, altered reaction kinetics compared to wild-type enzyme

758486

T24P

Candida albicans

Q5AAR6; Q5AB99

site-directed mutagenesis of the Naa20 subunit, altered reaction kinetics compared to wild-type enzyme

758486

T76A

Candida albicans

Q5AAR6; Q5AB99

site-directed mutagenesis of the Naa20 subunit, altered reaction kinetics compared to wild-type enzyme

758486

Y124F

Candida albicans

Q5AAR6; Q5AB99

site-directed mutagenesis of the Naa20 subunit, altered reaction kinetics compared to wild-type enzyme

758486

Y138A

Candida albicans

Q5AAR6; Q5AB99

site-directed mutagenesis of the Naa20 subunit, altered reaction kinetics compared to wild-type enzyme

758486

Y138A/Y139A

Candida albicans

Q5AAR6; Q5AB99

site-directed mutagenesis of the Naa20 subunit, altered reaction kinetics compared to wild-type enzyme

758486

Y138F

Candida albicans

Q5AAR6; Q5AB99

site-directed mutagenesis of the Naa20 subunit, altered reaction kinetics compared to wild-type enzyme

758486

Y139A

Candida albicans

Q5AAR6; Q5AB99

site-directed mutagenesis of the Naa20 subunit, altered reaction kinetics compared to wild-type enzyme

758486

Y139F

Candida albicans

Q5AAR6; Q5AB99

site-directed mutagenesis of the Naa20 subunit, altered reaction kinetics compared to wild-type enzyme

758486

Y362A

Candida albicans

Q5AAR6; Q5AB99

site-directed mutagenesis of the Naa25 subunit

758486

Y404A

Candida albicans

Q5AAR6; Q5AB99

site-directed mutagenesis of the Naa25 subunit

758486

F112A

Candida albicans ATCC MYA-2876

-

site-directed mutagenesis of the Naa20 subunit, altered reaction kinetics compared to wild-type enzyme

-

H74A

Candida albicans ATCC MYA-2876

-

site-directed mutagenesis of the Naa20 subunit, altered reaction kinetics compared to wild-type enzyme

-

Y124F

Candida albicans ATCC MYA-2876

-

site-directed mutagenesis of the Naa20 subunit, altered reaction kinetics compared to wild-type enzyme

-

Y138A

Candida albicans ATCC MYA-2876

-

site-directed mutagenesis of the Naa20 subunit, altered reaction kinetics compared to wild-type enzyme

-

R84A

Homo sapiens

-

the mutant has more than 80% less activity than the wild type enzyme

740688

Y124F

Homo sapiens

-

the mutant has about 90% less activity than the wild type enzyme

740688

additional information

12 entries

additional information

Candida albicans

Q5AAR6; Q5AB99

generation of a deletion mutant (residues 1-170) of the catalytic subunit Naa20. Structure of mutant NatB/Naa20 (residue 1-170) in complex with a peptide substrate MEAHNK-biotin and structure of NatB/Naa20 (full-length) in complex with a bisubstrate inhibitor CoA-MDSEVAALVID

758486

additional information

Candida albicans

-

generation of a deletion mutant (residues 1-170) of the catalytic subunit Naa20. Structure of mutant NatB/Naa20 (residue 1-170) in complex with a peptide substrate MEAHNK-biotin and structure of NatB/Naa20 (full-length) in complex with a bisubstrate inhibitor CoA-MDSEVAALVID

758486

additional information

Candida albicans ATCC MYA-2876

-

generation of a deletion mutant (residues 1-170) of the catalytic subunit Naa20. Structure of mutant NatB/Naa20 (residue 1-170) in complex with a peptide substrate MEAHNK-biotin and structure of NatB/Naa20 (full-length) in complex with a bisubstrate inhibitor CoA-MDSEVAALVID

-

additional information

Homo sapiens

P61599; Q14CX7

construction of NAA20-KO or NAA25-KO cells. Wild-type, NAA20-KO, or NAA25-KO cells are transfected with plasmids encoding PB2, PB1, PA, and NP, with a plasmid for the expression of viral RNA encoding the firefly luciferase, and with a plasmid encoding Renilla luciferase as a transfection control

756403

additional information

Mus musculus

P61600; Q8BWZ3

generation of Naa25-/- MEF cells and of NAA25-knockout mice

756931

additional information

Mus musculus

-

generation of Naa25-/- MEF cells and of NAA25-knockout mice

756931

additional information

Mus musculus C57BL/6

-

generation of Naa25-/- MEF cells and of NAA25-knockout mice

-

additional information

Saccharomyces cerevisiae

Q06504; Q12387

generation yeast naa20DELTA cells, W303 naa20DELTA, from wild-type strain W303-1B

756931

additional information

Saccharomyces cerevisiae

-

generation yeast naa20DELTA cells, W303 naa20DELTA, from wild-type strain W303-1B

756931

additional information

Saccharomyces cerevisiae

Q06504; Q12387

the wild-type yeast strain BY4743 transformed with the plasmid encoding wild-type influenza A PA-X, PA-X E2D, or PA-X E2N proteins barely formed any colonies, whereas wild-type yeast transformed with the plasmid encoding PA-X E2A or PA-X E2P viral proteins formed many colonies

756403

additional information

Saccharomyces cerevisiae ATCC 204508

-

the wild-type yeast strain BY4743 transformed with the plasmid encoding wild-type influenza A PA-X, PA-X E2D, or PA-X E2N proteins barely formed any colonies, whereas wild-type yeast transformed with the plasmid encoding PA-X E2A or PA-X E2P viral proteins formed many colonies

-

additional information

Saccharomyces cerevisiae ATCC 204508

-

generation yeast naa20DELTA cells, W303 naa20DELTA, from wild-type strain W303-1B

-