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2.3.1.212: benzalacetone synthase

This is an abbreviated version!
For detailed information about benzalacetone synthase, go to the full flat file.

Word Map on EC 2.3.1.212

Reaction

4-coumaroyl-CoA
+
malonyl-CoA
+
H2O
= 2 CoA +
4-hydroxybenzalacetone
+ 2 CO2

Synonyms

bAS, HsPKS3, PKS1, RinPKS1, RiPKS4, RpBAS

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.212 benzalacetone synthase

Purification

Purification on EC 2.3.1.212 - benzalacetone synthase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native enzyme 172fold from fruits by ammonium sulfate fractionation, gel filtration, hydrophobic interaction and anion exchange chromatography, and chromatofocusing
-
Ni-NTA column chromatography
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography and gel filtration
recombinant RiPKS4 from Escherichia coli strain BL21(DE3)pLYsS