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1.14.20.15: L-threonyl-[L-threonyl-carrier protein] 4-chlorinase

This is an abbreviated version!
For detailed information about L-threonyl-[L-threonyl-carrier protein] 4-chlorinase, go to the full flat file.

Word Map on EC 1.14.20.15

Reaction

an L-threonyl-[L-threonyl-carrier protein]
+
2-oxoglutarate
 +
O2
 +
Cl-
=
a 4-chloro-L-threonyl-[L-threonyl-carrier protein]
 +
succinate
 +
CO2
 +
H2O

Synonyms

aliphatic halogenase, SyrB2, syringomycin biosynthesis enzyme 2, Thr3

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.20 With 2-oxoglutarate as one donor, and the other dehydrogenated
                1.14.20.15 L-threonyl-[L-threonyl-carrier protein] 4-chlorinase

Engineering

Engineering on EC 1.14.20.15 - L-threonyl-[L-threonyl-carrier protein] 4-chlorinase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A118D
A118E
mutant enzyme binds Fe(II), but the mutations completely abrogates chlorination activity
A118Q
suppressed H-bonding interaction of Arg254 with Cl-Fe(III)-OH and suppressed chlorination activity
E102A
mutation abolishes the production of syringomycin, as shown by a lack of antifungal activity
F121A
mutation abolishes the production of syringomycin, as shown by a lack of antifungal activity
F195A
mutation abolishes the production of syringomycin, as shown by a lack of antifungal activity
F196A
inactive mutant enzyme
N123A
strong decrease in antifungal activity. The residual activity is approximately 26-30%
R247A
suppressed chlorination activity
R247Q
suppressed chlorination activity
R40A
mutant enzyme shows 26% residual activity
Y107A
mutant enzyme shows 75% residual activity
Y178A
mutant enzyme shows 23-26% activity
A118D
A118E
-
mutant enzyme binds Fe(II), but the mutations completely abrogates chlorination activity
-
A118Q
-
suppressed H-bonding interaction of Arg254 with Cl-Fe(III)-OH and suppressed chlorination activity
-
R247A
-
suppressed chlorination activity
-
R247Q
-
suppressed chlorination activity
-
E102A
-
mutation abolishes the production of syringomycin, as shown by a lack of antifungal activity
-
F196A
-
inactive mutant enzyme
-
R40A
-
mutant enzyme shows 26% residual activity
-
Y107A
-
mutant enzyme shows 75% residual activity
-
Y178A
-
mutant enzyme shows 23-26% activity
-
E105A
mutation abolishes the production of syringomycin, as shown by a lack of antifungal activity
F124A
mutation abolishes the production of syringomycin, as shown by a lack of antifungal activity
F195A
mutation abolishes the production of syringomycin, as shown by a lack of antifungal activity
F201A
inactive mutant enzyme
N126A
strong decrease in antifungal activity. The residual activity is approximately 26-30%
R44A
mutant enzyme shows 26% residual activity
Y110A
mutant enzyme shows 74% residual activity
Y184A
mutant enzyme shows 23-26% activity