1.1.1.430: D-xylose reductase (NADH)
This is an abbreviated version!
For detailed information about D-xylose reductase (NADH), go to the full flat file.
Reaction
Synonyms
CbXR, CT-XR, dsXR, NAD(P)H-dependent D-xylose reductase, NADH-dependent XR, NADPH-preferring xylose reductase, PsXR, XYL1, XYL1.1, XYL1.2, xylose reductase
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Cofactor
Cofactor on EC 1.1.1.430 - D-xylose reductase (NADH)
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NADH
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dual specific xylose reductase (dsXR) has an about 4fold higher specificity for NADH than NADPH
NADH
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transient-state and steady-state kinetic studies of the mechanism of NADH-dependent aldehyde reduction
NADH
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using a modified iterative protein redesign and optimization workflow, a sets of mutations is identified that change the nicotinamide cofactor specificity of xylose reductase (CbXR) from its physiological preference for NADPH, to the alternate cofactor NADH
NADH
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wild-type enzyme prefers NADPH over NADH. Mutant enzyme K270S/N272P/S271G/R276F shows a 25fold preference toward NADH over NADPH by a factor of about 13fold, or an improvement of about 42fold, as measured by the ratio of the specificity constant kcat/Km coenzyme
NADPH
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dual specific xylose reductase (dsXR) has an about 4fold higher specificity for NADH than NADPH. NADPH-dependent monospecific xylose reductase (msXR) shows non activity with NADH
NADPH
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using a modified iterative protein redesign and optimization workflow, a sets of mutations is identified that change the nicotinamide cofactor specificity of xylose reductase (CbXR) from its physiological preference for NADPH, to the alternate cofactor NADH
NADPH
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wild-type enzyme prefers NADPH over NADH. Mutant enzyme K270S/N272P/S271G/R276F shows a 25fold preference toward NADH over NADPH by a factor of about 13fold, or an improvement of about 42fold, as measured by the ratio of the specificity constant kcat/Km coenzyme
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Kluyveromyces marxianus strains expressing Pichia stipitis Psxyl1 genes show reversed cofactor specificity, overview
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additional information
most XRs are NADPH-dependent rather than NADH-dependent. CT-XR from Candida tenuis shows a similar preference for both NADH and NADPH
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