Literature summary for 1.1.1.430 extracted from

Petschacher, B.; Nidetzky, B.
Altering the coenzyme preference of xylose reductase to favor utilization of NADH enhances ethanol yield from xylose in a metabolically engineered strain of Saccharomyces cerevisiae (2008), Microb. Cell Fact., 7, 9.

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Cloned(Commentary)

Cloned (Comment)	Organism
overexpression of wild-type enzyme and mutant enzyme, with modified cofactor specificity, in Saccharomyces cerevisiae strain CEN.PK 113-7D under control of the constitutive TDH3 promoter, coexpression with xylitol dehydrogenase from Galactocandida mastotermitis, The strain harboring the xylose reductase double mutant shows 42% enhanced ethanol yield compared to the reference strain harboring wild-type xylose reductase during anaerobic bioreactor conversions of xylose. Likewise, the yields of xylitol and glycerol are decreased by 52% and 57% respectively in the xylose reductase mutant strain	Yamadazyma tenuis

Cloned (Comment)

Organism

overexpression of wild-type enzyme and mutant enzyme, with modified cofactor specificity, in Saccharomyces cerevisiae strain CEN.PK 113-7D under control of the constitutive TDH3 promoter, coexpression with xylitol dehydrogenase from Galactocandida mastotermitis, The strain harboring the xylose reductase double mutant shows 42% enhanced ethanol yield compared to the reference strain harboring wild-type xylose reductase during anaerobic bioreactor conversions of xylose. Likewise, the yields of xylitol and glycerol are decreased by 52% and 57% respectively in the xylose reductase mutant strain

Yamadazyma tenuis

Protein Variants

Protein Variants	Comment	Organism
K274R/N276D	structure-guided site-directed mutagenesis, change of the coenzyme preference of the xyluose reductase about 170fold from NADPH in the wild-type to NADH, which, in spite of the structural modifications introduced, has retained the original catalytic efficiency for reduction of xylose by NADH	Yamadazyma tenuis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-xylose + NAD(P)H + H+	Yamadazyma tenuis	-	xylitol + NAD(P)+	-	?

Organism

Organism	UniProt	Comment	Textmining
Yamadazyma tenuis	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-xylose + NAD(P)H + H+	-	Yamadazyma tenuis	xylitol + NAD(P)+	-	?

Synonyms

Synonyms	Comment	Organism
xylose reductase	-	Yamadazyma tenuis

Cofactor

Cofactor	Comment	Organism	Structure
NAD(P)H	-	Yamadazyma tenuis