EC Number   |
Protein Variants |
Reference |
|---|
   3.4.21.59 | D215G |
site-directed mutagenesis, derivative of tryptase alpha and beta/II |
651932 |
| 3.4.21.59 | D216G |
active site variant of rHTalpha |
650140 |
| 3.4.21.59 | D216G |
site-directed mutagenesis, alphaI-tryptase mutant, crystal structure determination and analysis, the sole mutation of Asp216 to Gly produces a protease with catalytic and instability properties intermediate between those of alpha and beta-tryptases |
683768 |
| 3.4.21.59 | E149T |
active site variant of rHTbeta |
650140 |
| 3.4.21.59 | G215D |
the mutation distorts the substrate binding site and limits peptidase activity |
708858 |
| 3.4.21.59 | I16G |
the inactive mutant cannot tetramerize |
754178 |
| 3.4.21.59 | I99C |
the mutant shows 33% of wild type activity |
754178 |
| 3.4.21.59 | I99C*/Y75A/Y37bA |
the monomeric mutant (where C* is cysteinylated Cys-99) cannot form a dimer or tetramer, yet it is active (2.9% of wild type activity) but only in the presence of heparin |
754178 |
| 3.4.21.59 | I99C/Y75A/Y37bA |
the dimeric mutant shows 2.1% of wild type activity |
754178 |
| 3.4.21.59 | K192Q |
active site variant of rHTalpha |
650140 |
