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Literature summary for 3.4.21.59 extracted from

  • Maun, H.R.; Liu, P.S.; Franke, Y.; Eigenbrot, C.; Forrest, W.F.; Schwartz, L.B.; Lazarus, R.A.
    Dual functionality of beta-tryptase protomers as both proteases and cofactors in the active tetramer (2018), J. Biol. Chem., 293, 9614-9628 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.59

Activating Compound

Activating Compound Comment Organism Structure
heparin heparin both stabilizes the tetramer and allosterically conditions the active site Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in baculovirus-infected Trichoplusia ni insect cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant I99C, vapor diffusion method Homo sapiens

Protein Variants

Protein Variants Comment Organism
I16G the inactive mutant cannot tetramerize Homo sapiens
I99C the mutant shows 33% of wild type activity Homo sapiens
I99C*/Y75A/Y37bA the monomeric mutant (where C* is cysteinylated Cys-99) cannot form a dimer or tetramer, yet it is active (2.9% of wild type activity) but only in the presence of heparin Homo sapiens
I99C/Y75A/Y37bA the dimeric mutant shows 2.1% of wild type activity Homo sapiens
S195A the mutant is catalytically incompetent in its protease form Homo sapiens
Y75C the mutant shows 63% of wild type activity Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Glu-Gly-Arg chloromethyl ketone
-
 Homo sapiens
additional information aprotinin only inhibits monomeric beta-tryptase mutant Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.32
-
 S-2288 mutant enzyme Y75C, at pH 6.8 and 25°C Homo sapiens
0.47
-
 S-2288 wild type enzyme, at pH 6.8 and 25°C Homo sapiens
0.82
-
 S-2288 mutant enzyme I99C, at pH 6.8 and 25°C Homo sapiens
1
-
 S-2288 mutant enzyme I99C/Y75A/Y37bA, at pH 6.8 and 25°C Homo sapiens
1.6
-
 S-2288 mutant enzyme I99C*/Y75A/Y37bA, at pH 6.8 and 25°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
147000
-
 enzyme in complex with murine B12 Fab, gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q15661
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and S200 gel filtration Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
mast cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-2288 + H2O
-
 Homo sapiens D-Ile-L-Pro-L-Arg + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
tetramer
-
 Homo sapiens

Synonyms

Synonyms Comment Organism
beta-tryptase isoform Homo sapiens
betaI-tryptase
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.8
-
 S-2288 mutant enzyme I99C/Y75A/Y37bA, at pH 6.8 and 25°C Homo sapiens
6.7
-
 S-2288 mutant enzyme I99C*/Y75A/Y37bA, at pH 6.8 and 25°C Homo sapiens
37
-
 S-2288 mutant enzyme Y75C, at pH 6.8 and 25°C Homo sapiens
50
-
 S-2288 mutant enzyme I99C, at pH 6.8 and 25°C Homo sapiens
87
-
 S-2288 wild type enzyme, at pH 6.8 and 25°C Homo sapiens

General Information

General Information Comment Organism
physiological function the enzyme is an important mediator of the allergic inflammatory responses in asthma Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4
-
 S-2288 mutant enzyme I99C*/Y75A/Y37bA, at pH 6.8 and 25°C Homo sapiens
6
-
 S-2288 mutant enzyme I99C/Y75A/Y37bA, at pH 6.8 and 25°C Homo sapiens
61
-
 S-2288 mutant enzyme I99C, at pH 6.8 and 25°C Homo sapiens
120
-
 S-2288 mutant enzyme Y75C, at pH 6.8 and 25°C Homo sapiens
180
-
 S-2288 wild type enzyme, at pH 6.8 and 25°C Homo sapiens