2.1.1.246: [methyl-Co(III) methanol-specific corrinoid protein]:coenzyme M methyltransferase
This is an abbreviated version!
For detailed information about [methyl-Co(III) methanol-specific corrinoid protein]:coenzyme M methyltransferase, go to the full flat file.
Word Map on EC 2.1.1.246
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2.1.1.246
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methyltransferases
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euchromatic
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methanosarcina
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barkeri
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rna-directed
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methanogenesis
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monomethylamine
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24-nucleotide
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dimethylamine
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biofuel production
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synthesis
- 2.1.1.246
- methyltransferases
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euchromatic
- methanosarcina
- barkeri
-
rna-directed
-
methanogenesis
- monomethylamine
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24-nucleotide
- dimethylamine
- biofuel production
- synthesis
Reaction
Synonyms
Co-methyl-5-hydroxybenzimidazolylcobamide:2-mercapto-ethanesulphonic acid methyltransferase, methanol-specific methyltransferase 2, methanol:cob(I)alamin methyltransferase, methanol:coenzyme M methyltransferase, methylcobalamin:coenzyme M methyltransferase, methylcobamide:coenzyme M methyltransferase, methylcobamide:CoM methyltransferase, methyltransferase 2, MT2, MT2-A, MT2-M, mtaA
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Substrates Products
Substrates Products on EC 2.1.1.246 - [methyl-Co(III) methanol-specific corrinoid protein]:coenzyme M methyltransferase
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REACTION DIAGRAM
a [methyl-Co(III) methanol-specific corrinoid protein] + 3-mercaptopropanoic acid
methyl-3-mercaptopropanoic acid + a [Co(I) methanol-specific corrinoid protein]
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r
a [methyl-Co(III) methanol-specific corrinoid protein] + coenzyme M
methyl-CoM + a [Co(I) methanol-specific corrinoid protein]
a [methyl-Co(III) methanol-specific corrinoid protein] + CoM
methyl-CoM + a [Co(I) methanol-specific corrinoid protein]
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?, r
methyl-CoM + a [Co(I) methanol-specific corrinoid protein]
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a [methyl-Co(III) methanol-specific corrinoid protein] + coenzyme M
methyl-CoM + a [Co(I) methanol-specific corrinoid protein]
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r
a [methyl-Co(III) methanol-specific corrinoid protein] + coenzyme M
methyl-CoM + a [Co(I) methanol-specific corrinoid protein]
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r
a [methyl-Co(III) methanol-specific corrinoid protein] + coenzyme M
methyl-CoM + a [Co(I) methanol-specific corrinoid protein]
the enzyme methylcobamide:CoM methyltransferase, MT2, catalyzes the transfer of the methyl group from the MT1-bound methylcobamide prosthetic group to coenzyme M
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r
a [methyl-Co(III) methanol-specific corrinoid protein] + coenzyme M
methyl-CoM + a [Co(I) methanol-specific corrinoid protein]
Methanosarcina barkeri Fusaro / DSM 804
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r
a [methyl-Co(III) methanol-specific corrinoid protein] + coenzyme M
methyl-CoM + a [Co(I) methanol-specific corrinoid protein]
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r
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methyltransferase 2, MT2, transfers the methyl group from the methylated corrinoid protein to coenzyme M, i.e. 2-mercaptoethanesulfonic acid. Methyl-coenzyme M is then disproportionated, with one molecule being oxidized to CO2 to provide the six electrons required for reduction of three additional methyl-coenzyme M molecules to methane
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additional information
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transferase MtaA catalyzes the transfer of the methyl group from methylated transferase MtaBC to coenzyme M. Instead of methylated transferase MtaBC, MtaA can also use free methylcobalamin as the methyl donor. MtaA activates coenzyme M for nucleophilic attack of the methyl group of the methylcobamide
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additional information
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conversions of monomethylamine and dimethylamine to CH3-SCoM are dependent upon MT2-A, and are not supported by MT2-M. In contrast, MT2-M acts specifically in metabolism of methanol, but does not substitute for MT2-A in conversion of monomethylamine or dimethylamine. Nevertheless, both isozymes are capable of supporting the conversion of trimethylamine
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additional information
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conversions of monomethylamine and dimethylamine to CH3-SCoM are dependent upon MT2-A, and are not supported by MT2-M. In contrast, MT2-M acts specifically in metabolism of methanol, but does not substitute for MT2-A in conversion of monomethylamine or dimethylamine. Nevertheless, both isozymes are capable of supporting the conversion of trimethylamine
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additional information
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in the assay for methanol:coenzyme M methyltransferase activity cob(I)alamin can be substituted by cob(I)inamide which is devoid of the nucleotide loopmethylation of cob(I)inamide with methanol is dependent on imidazole but not on the demethylation of methylcob(III)inamide with coenzyme M
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additional information
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recombinant MtaA catalyzes the formation of methyl-coenzyme M from free CH3-cob(III)alamin and coenzyme M at specific rates comparable to those predicted for MtaA-catalyzed MtaC demethylation
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additional information
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the reaction for possible production of methanol from the anaerobic oxidation of methane can be reversed in vitro. Develoment of an in vitro functional assay that demonstrates MtaABC can catalyze the energetically unfavorable reverse reaction starting from methyl coenzyme M and generating methanol as a product, overview. MtaABC enzyme protein complex to catalyze the methyl transfer from methanol to CoM to form methyl-CoM, which is energetically favorable in the forward reaction. MtaB catalyzes a methyl transfer from methanol to the corrinoid cofactor of the MtaC subunit. MtaA then catalyzes the transfer of the methyl group to CoM to form methyl-CoM. DTNB assays for the forward and reverse MtaABC reactions. Methylcobalamin assay of purified recombinant MtaA.Demethylation of methylcobalamin in a CoM-dependent manner by MtaA
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