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2.1.1.229: tRNA (carboxymethyluridine34-5-O)-methyltransferase

This is an abbreviated version!
For detailed information about tRNA (carboxymethyluridine34-5-O)-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.229

Reaction

S-adenosyl-L-methionine
+
carboxymethyluridine34 in tRNA
=
S-adenosyl-L-homocysteine
 +
5-(2-methoxy-2-oxoethyl)uridine34 in tRNA

Synonyms

ABH8, ALKBH8, CmoA, MnmC, Trm9, Trm9-Trm112, Trm9p, TrmC, tRNA methyltransferase 9, YfcK, YML014w

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.229 tRNA (carboxymethyluridine34-5-O)-methyltransferase

Purification

Purification on EC 2.1.1.229 - tRNA (carboxymethyluridine34-5-O)-methyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
co-purification of His-tagged Trm9p with untagged Trm112p as a complex from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography
-
recombinant His-tagged enzyme from Escherichia coli strain Rosetta pLysS (DE3) by nickel affinity chromatography and gel filtration, followed by cleavage of the N-terminal His6-tag with rhinovirus 3C protease and another step of nickel affinity chromatography to remove the tag
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage with thrombin , and again nickel adffinity chromatography for tag removal, followed by gel filtration
-
recombinant N-terminally His6-tagged wild-type and selenomethionine-labeled enzyme from Escherichia coli strains by nickel affinity chromatography, tag cleavage with enterokinase, and again nickel adffinity chromatography for tag removal, followed by gel filtration
recombinant wild-type and selenomethinone-labeled enzymes from Escherichia coli by ultracentrifugation, anion exchange and hydrophobic interaction chromatography, another step of anion exchange chromatography and gel filtration