2.1.1.218: tRNA (adenine9-N1)-methyltransferase
This is an abbreviated version!
For detailed information about tRNA (adenine9-N1)-methyltransferase, go to the full flat file.
Reaction
Synonyms
adenosine-specific Trm10, EC 2.1.1.36, hTRMT10B, m1A9 MTase, m1R9-specific TkTrm10, More, SaTrm10, SPOUT MTase, TK0422p, TkTrm10, Trm10, Trm10p (ambiguous), TRMT10B, Trmt10C, tRNA m1A9 methyltransferase, tRNA m1A9 MTase, tRNA m1A9-methyltransferase, tRNA m1R9 methyltransferase, tRNA(m1G9/m1A9)-methyltransferase, tRNA(m1G9/m1A9)MTase
ECTree
2.1.1.218 tRNA (adenine9-N1)-methyltransferaseAdvanced search results
results (
results (Engineering
Engineering on EC 2.1.1.218 - tRNA (adenine9-N1)-methyltransferase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
top
PROTEIN VARIANTS 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
G231R
site-directed mutagenesis, the mutation abolishs methylation of tRNAAsp and tRNATrp, with some residual (though reduced) activity on tRNAArg
G231R/G232R
site-directed mutagenesis, the double mutant displays no methylation activity on any of the three substrates
D184N
site-directed mutagenesis, kinetics and structure comparison
D220N
site-directed mutagenesis, kinetics and structure comparison
K121E
site-directed mutagenesis, the mutant shows highly reduced activity compared to wild-type
K185E
site-directed mutagenesis, the mutant shows very highly reduced activity compared to wild-type
K249E
site-directed mutagenesis, the mutant is almost inactive
K38E
site-directed mutagenesis, the mutant shows very highly reduced activity compared to wild-type
K5E
site-directed mutagenesis, the mutant shows moderately reduced activity compared to wild-type
K64E
site-directed mutagenesis, the mutant shows highly reduced activity compared to wild-type
K75E
site-directed mutagenesis, the mutant shows very highly reduced activity compared to wild-type
K78E
site-directed mutagenesis, the mutant shows moderately reduced activity compared to wild-type
R276E
site-directed mutagenesis, the mutant shows moderately reduced activity compared to wild-type
R288E
site-directed mutagenesis, the mutant is almost inactive
R47E
site-directed mutagenesis, the mutant shows very highly reduced activity compared to wild-type
R74E
site-directed mutagenesis, the mutant shows very highly reduced activity compared to wild-type
D220N
-
site-directed mutagenesis, kinetics and structure comparison
-
K75E
-
site-directed mutagenesis, the mutant shows very highly reduced activity compared to wild-type
-
K78E
-
site-directed mutagenesis, the mutant shows moderately reduced activity compared to wild-type
-
R276E
-
site-directed mutagenesis, the mutant shows moderately reduced activity compared to wild-type
-
R47E
-
site-directed mutagenesis, the mutant shows very highly reduced activity compared to wild-type
-
D104A
site-directed mutagenesis, the mutant shows moderately reduced activity compared to wild-type
D104A/E115Q/D245A
site-directed mutagenesis, almost inactive mutant
D104N
site-directed mutagenesis, the mutant shows slightly reduced activity compared to wild-type
D104N/D206N/D245N
site-directed mutagenesis, the mutant shows highly reduced activity compared to wild-type
D104N/D206N/D245N/E115Q
site-directed mutagenesis, the mutant shows highly reduced activity compared to wild-type
D206A
site-directed mutagenesis, the enzyme activity is modestly reduced compared to wild-type
D206A/D245A
site-directed mutagenesis, the enzyme activity is abolished in the double mutant
D206N
site-directed mutagenesis, the mutant shows moderately reduced activity compared to wild-type
D245A
site-directed mutagenesis, the enzyme activity is modestly reduced compared to wild-type
D245N
site-directed mutagenesis, the mutation has no significant effect on the A-preference for TktRNAAsp, but exhibits a modest, but shows about 4fold reduced G-preference activity compared to wild-type
E115Q
site-directed mutagenesis, the mutant shows moderately reduced activity compared to wild-type
G202R
site-directed mutagenesis, the mutant is nearly inactive, nearly complete loss of both m1G9 and m1A9 activity
G242R
site-directed mutagenesis, the mutant shows unaltered activity
Q122A
site-directed mutagenesis, the mutant shows highly reduced activity compared to wild-type
