2.1.1.218: tRNA (adenine9-N1)-methyltransferase
This is an abbreviated version!
For detailed information about tRNA (adenine9-N1)-methyltransferase, go to the full flat file.
Reaction
Synonyms
adenosine-specific Trm10, EC 2.1.1.36, hTRMT10B, m1A9 MTase, m1R9-specific TkTrm10, More, SaTrm10, SPOUT MTase, TK0422p, TkTrm10, Trm10, Trm10p (ambiguous), TRMT10B, Trmt10C, tRNA m1A9 methyltransferase, tRNA m1A9 MTase, tRNA m1A9-methyltransferase, tRNA m1R9 methyltransferase, tRNA(m1G9/m1A9)-methyltransferase, tRNA(m1G9/m1A9)MTase
ECTree
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Crystallization
Crystallization on EC 2.1.1.218 - tRNA (adenine9-N1)-methyltransferase
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purified recombinant truncated construct of SaTrm10, lacking the C-terminal domain, native and enriched in selenomethionine (SaTrm10 1-249 and SaTrm10 1-249 SAD, respectively), hanging drop vapour diffusion, for SaTrm10 1-249: mixing of 15 mg/ml protein solution with an equal volume of crystallisation buffer containing 8% ethylene glycol, 0.1 M HEPES, pH 7.5, and 10% PEG 8000, for SaTrm10 1-249 SAD: mixing of 15 mg/ml protein solution with an equal volume of crystallisation buffer containing 2% dioxane, 0.05 M bicine, pH 9.0, and 15% PEG 20000, for full-length enzyme: mixing of 8 mg/ml protein in the presence of 1 mM SAH with an equal volume of crystallisation buffer containing 0.4 M (NH4)2SO4, 0.1 M sodium acetate, pH 4.5, and 15-20% PEG2000MME, several months, 20°C, X-ray diffraction structure determination and analysis at 2.1-2.4 A resolution, molecular replacement and modelling