1.14.20.7: 2-oxoglutarate/L-arginine monooxygenase/decarboxylase (succinate-forming)
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For detailed information about 2-oxoglutarate/L-arginine monooxygenase/decarboxylase (succinate-forming), go to the full flat file.
Reaction
Synonyms
2-oxoglutarate-dependent ethylene/succinate-forming enzyme, 2-oxoglutarate-dependent oxygenase-type ethylene-forming-enzyme, 2-oxoglutarate-Fe(II) oxygenase, 2OG-Fe(II) oxygenase, EC 1.14.11.34, EFE, ethylene forming enzyme, ethylene-forming enzyme, More
ECTree
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Purification
Purification on EC 1.14.20.7 - 2-oxoglutarate/L-arginine monooxygenase/decarboxylase (succinate-forming)
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recombinant His-tagged enzyme from Escherichia coli strain BL21 Gold (DE3) by nickel affinity chromatography, tag cleavage by a TEV protease mutant, and another step of nickel affinity chromatography, and dialysis of the flow through
recombinant N-terminally His6-tagged enzyme from Escherichia coli by nickel affinity chromatography
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