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1.14.20.7: 2-oxoglutarate/L-arginine monooxygenase/decarboxylase (succinate-forming)

This is an abbreviated version!
For detailed information about 2-oxoglutarate/L-arginine monooxygenase/decarboxylase (succinate-forming), go to the full flat file.

Reaction

5-hydroxy-L-arginine
=
guanidine
 +
(S)-1-pyrroline-5-carboxylate
 +
H2O

Synonyms

2-oxoglutarate-dependent ethylene/succinate-forming enzyme, 2-oxoglutarate-dependent oxygenase-type ethylene-forming-enzyme, 2-oxoglutarate-Fe(II) oxygenase, 2OG-Fe(II) oxygenase, EC 1.14.11.34, EFE, ethylene forming enzyme, ethylene-forming enzyme, More

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.20 With 2-oxoglutarate as one donor, and the other dehydrogenated
                1.14.20.7 2-oxoglutarate/L-arginine monooxygenase/decarboxylase (succinate-forming)

Cloned

Cloned on EC 1.14.20.7 - 2-oxoglutarate/L-arginine monooxygenase/decarboxylase (succinate-forming)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli MG1655 cells
-
expressed in Saccharomyces cerevisiae strain SCNJ03
-
expression in Escherichia coli
gene efe, codon optimization, gene synthesis, and construction of the self-replicating wide-host-range pDF-series vectors, recombinant expression in Synechocystis sp. strain PCC 6803, the cyanobacterial system is highly unstable resulting in rapid development of mutants that lost the capability to synthesize ethylene, N-terminally His6-tagged enzyme expression in Escherichia coli. Ethylene synthesis in Escherichia coli and Synechocystis using three different metal-inducible promoters from cyanobacteria, expression rates and genomic structure of the native metal-inducible promoter elements, method development, overview
-
gene efe, large scale expression of His6-tagged enzyme in Escherichia coli strain BL21 Gold (DE3), method optimization and evaluation
gene efe, recombinant expression in Escherichia coli strain MG1655, importance of promoter strength on the expression of enzyme EFE in Escherichia coli with stronger promoters producing elevated levels of ethylene, e.g. the Amaranthus hybridus chloroplast psbA promoter (PpsbA), expression method optimization, overview
-
recombinant expression of wild-type and mutant enzymes