1.14.19.65: (S)-cheilanthifoline synthase

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For detailed information about (S)-cheilanthifoline synthase, go to the full flat file.

Reaction

Synonyms

(S)-scoulerine oxidase (methylenedioxy-bridge-forming), cheilanthifoline synthase, CYP719A13, CYP719A14, CYP719A5, EC 1.1.3.33, EC 1.14.21.2, S-cheilanthifoline synthase, synthase, (S)-cheilanthifoline

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.19 With oxidation of a pair of donors resulting in the reduction of O₂ to two molecules of water

                1.14.19.65 (S)-cheilanthifoline synthase

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1	Activating Compound
4	AA Sequence
2	Application
1	CAS Registry Number
4	Cloned(Commentary)
3	Cofactor
1	Expression
2	General Information
3	KM Value [mM]
1	Localization
1	Molecular Weight [Da]
4	Natural Substrates/ Products (Substrates)
6	Organism
5	Pathway
4	pH Optimum
3	pH Range
1	Purification (Commentary)
1	Reaction
5	Reaction Type
6	Reference
7	Source Tissue
1	Storage Stability
9	Substrates and Products (Substrate)
13	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
1	Temperature Range [°C]
2	Temperature Stability [°C]
3	Turnover Number [1/s]

Cloned

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Cloned on EC 1.14.19.65 - (S)-cheilanthifoline synthase

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

coexpression of cheilanthifoline synthase (CYP719A5), and stylopine synthase (CYP719A2) in Pichia pastoris. Biosynthetic enzyme expression is examined in a consolidated system with all genes expressed in one cell and a coculture system with three cell lines that each express a single gene were examined. Although both systems efficiently converted reticuline to stylopine, the consolidated system is more rapid and efficient than the co-culture system. However, substrate-feeding experiments reveal a decrease in the conversion efficiency in the consolidated system during successive cultures, whereas the conversion efficiency in the co-culture system remains constant. Thus, the final amount of stylopine produced from reticuline after successive feedings in the co-culture system is more than 150 nmoles from 750 nmoles of (R,S)-reticuline (375 nmoles of (S)-reticuline)

Eschscholzia californica

B5UAQ8

746447

expressed in Spodoptera frugiperda Sf9 cells

Argemone mexicana

B1NF19

711025

heterologously expressed in Saccharcomyces cerevisiae

Chelidonium majus

A0A240FWB3

746047

into the pT7Blue T-vector for sequencing, and subcloned into a yeast expression vector

Eschscholzia californica

-

700725