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1.14.19.58: tryptophan 5-halogenase

This is an abbreviated version!
For detailed information about tryptophan 5-halogenase, go to the full flat file.

Word Map on EC 1.14.19.58

Reaction

L-tryptophan
+
FADH2
 +
chloride
 +
O2
 +
H+
=
5-chloro-L-tryptophan
 +
FAD
 + 2 H2O

Synonyms

halogenase, mibH, pyrH, sttH, trp-5-halogenase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.19 With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
                1.14.19.58 tryptophan 5-halogenase

Crystallization

Crystallization on EC 1.14.19.58 - tryptophan 5-halogenase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified recombinant His6-tagged enzyme with bound FAD, hanging drop vapour diffusion method, mixing of 22 mg/ml protein in 20 mM HEPES, pH 8.0, and 150 mM KCl, with reservoir solution containing 12.5% MPD, 12.5% PEG 3350, 0.1 M MES/imidazole, pH 6.5, and 0.09 M NPS (1:1:1 NaNO3, Na2HPO4, (NH4)2SO4), X-ray diffraction structure determination and analysis at 1.85 A resolution
sitting-drop vapor-diffusion method at 20°C, crystal structure of a tryptophan 5-halogenase (PyrH) bound to tryptophan and FAD