1.14.14.90: isoflavone 2'-hydroxylase

This is an abbreviated version!
For detailed information about isoflavone 2'-hydroxylase, go to the full flat file.

Word Map on EC 1.14.14.90

1.14.14.90

isoflavonoids

phytoalexins

glycyrrhiza

flavonoid

echinata

genistein

cinnamic

4-hydroxylase

phenylpropanoids

chickpea

medicarpin

cicer

elicitor

legume

licorice

2'-hydroxylated

arietinum

Reaction

Synonyms

AmI2'H, CYP Ge-3, CYP81E, CYP81E1, CYP81E11, CYP81E12, CYP81E13, CYP81E42, EC 1.14.13.89, I2'H, isoflavone 2'-monooxygenase, LjCY-2 protein, MtCYP81E7

ECTree

1 Oxidoreductases

1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor

1.14.14.90 isoflavone 2'-hydroxylase

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Results	in table
1	Activating Compound
21	AA Sequence
1	CAS Registry Number
8	Cloned(Commentary)
7	Cofactor
1	Expression
2	General Information
2	KM Value [mM]
2	Localization
7	Natural Substrates/ Products (Substrates)
10	Organism
6	Pathway
1	pH Optimum
1	Protein Variants
1	Purification (Commentary)
1	Reaction
9	Reference
5	Source Tissue
22	Substrates and Products (Substrate)
1	Subunits
20	Synonyms
1	Systematic Name
2	Turnover Number [1/s]

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Reference on EC 1.14.14.90 - isoflavone 2'-hydroxylase

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285323

Akashi, T.; Aoki, T.; Ayabe, S.I.

CYP81E1, a cytochrome P450 cDNA of licorice (Glycyrrhiza echinata L.), encodes isoflavone 2'-hydroxylase

Biochem. Biophys. Res. Commun.

251

67-70

1998

Glycyrrhiza echinata (P93147), Glycyrrhiza echinata L. (P93147)

9790908

285324

Shimada, N.; Akashi, T.; Aoki, T.; Ayabe, S.i.

Induction of isoflavonoid pathway in the model legume Lotus japonicus: molecular characterization of enzymes involved in phytoalexin biosynthesis

Plant Sci.

160

37-47

2000

Lotus japonicus

11164575

658293

Nakamura, K.; Akashi, T.; Aoki, T.; Kawaguchi, K.; Ayabe, S.

Induction of isoflavonoid and retrochalcone branches of the flavonoid pathway in cultured Glycyrrhiza echinata cells treated with yeast extract

Biosci. Biotechnol. Biochem.

1618-1620

1999

Glycyrrhiza echinata (P93147), Glycyrrhiza echinata, Glycyrrhiza echinata AK-1 (P93147)

10540749

660173

Liu, C.J.; Huhman, D.; Sumner, L.W.; Dixon, R.A.

Regiospecific hydroxylation of isoflavones by cytochrome p450 81E enzymes from Medicago truncatula

Plant J.

471-484

2003

Medicago truncatula (Q6WNR0), Medicago truncatula

14617078

745618

Gholampour, H.; Ghayeb Zamharir, M.; Karimi, J.; Farrokhi, N.; Alizadeh, A.; Taheri, P.

Identification of genes expressed differentially in grapefruit infected with Candidatus liberibacter asiaticus in the late stage of disease

J. Phytopathol.

162

811-819

2014

Citrus x paradisi

746010

Uchida, K.; Akashi, T.; Aoki, T.

Functional expression of cytochrome P450 in Escherichia coli An approach to functional analysis of uncharacterized enzymes for flavonoid biosynthesis

Plant Biotechnol.

205-213

2015

Glycine max, Glycine max (Q2LAL0)

746367

Chen, J.; Yuan, H.; Zhang, L.; Pan, H.; Xu, R.; Zhong, Y.; Chen, J.; Nan, P.

Cloning, expression and purification of isoflavone-2'-hydroxylase from Astragalus membranaceus Bge. Var. mongolicus (Bge.) Hsiao

Protein Expr. Purif.

107

83-89

2015

Astragalus membranaceus (W5S082), Astragalus membranaceus

25462811