Cloned (Comment) | Organism |
---|---|
gene AmI2'H or CYP81E42, DNA and amino acid sequence determination and analysis, recombinant expression of truncated enzyme, lacking the N-terminal membrane binding motif, in Escherichia coli strain BL21(DE3). Expression of the enzyme without removal of the transmembrane domain or insertion of the hydrophilic peptide AKKTSSKGKL is unsuccessful | Astragalus membranaceus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | modification of the gene sequence to facilitate protein expression and increase protein solubility, removal of the N-terminal membrane binding motif to enhance protein solubility | Astragalus membranaceus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | transmembrane enzyme | Astragalus membranaceus | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Astragalus membranaceus | W5S082 | var. mongolicus | - |
Purification (Comment) | Organism |
---|---|
recombinant truncated enzyme, lacking the N-terminal membrane binding motif, from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Astragalus membranaceus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | AmI2'H is expressed throughout the entire plant | Astragalus membranaceus | - |
Subunits | Comment | Organism |
---|---|---|
More | secondary structure analysis of AmI2'H | Astragalus membranaceus |
Synonyms | Comment | Organism |
---|---|---|
AmI2'H | - |
Astragalus membranaceus |
CYP81E42 | - |
Astragalus membranaceus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P-450 | - |
Astragalus membranaceus | |
NADPH-hemoprotein reductase | A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._ | Astragalus membranaceus |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in isoflavonoid biosynthesis | Astragalus membranaceus |
physiological function | the enzyme promotes the accumulation of medicarpin malonyl glucoside | Astragalus membranaceus |