EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
---|
1.17.1.9 | 4 |
50 |
the Escherichia coli cell-surface-displayed enzyme retains its original enzymatic activity after incubation at 4°C for 1 month with the half-life of 17 days at 40°C and 38 h at 50°C |
740397 |
1.17.1.9 | 25 |
48 |
melting temperature is 48°C. The enzyme remains stable for up to 30 min at 25°C |
743715 |
1.17.1.9 | 25 |
50 |
the enzyme remains stable from 25-50°C and rapidly loses activity at higher temperatures (inactive at 60°C) |
725735 |
1.17.1.9 | 30 |
- |
pH 5.2-9.0, 1 h, more than 90% of the activity is retained |
288104 |
1.17.1.9 | 30 |
40 |
the enzyme remains stable after 1 h at 30-40°C |
739921 |
1.17.1.9 | 30 |
52 |
the enzyme remains stable after 20 min at 30-52°C. The half-life temperature is 56.7°C. The enzyme is inactivated after 20 min at 60°C |
739917 |
1.17.1.9 | 35 |
- |
10 min, stable |
288120 |
1.17.1.9 | 37 |
- |
almost complete inactivation in 2-3 days, -SH compounds protect against inactivation, NAD+ or NADH decreases the inactivation rate, inactivation rate is significantly diminished in presence of formate, strongest stabilization by EDTA |
288086 |
1.17.1.9 | 40 |
- |
after 2 h at 40°C and 60°C, the wild type enzyme retains 44% and 10% of its initial activity, respectively |
740308 |
1.17.1.9 | 40 |
60 |
the enzyme retains 93, 88, 83, and 71% of its initial activity after 4 h of exposure at 40, 50, 55, and 60 °C, respectively |
724094 |