Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | [Candida] boidinii |
Protein Variants | Comment | Organism |
---|---|---|
N187D | the mutant shows increased catalytic efficiency compared to the wild type enzyme | [Candida] boidinii |
V120S | the mutant has the highest catalytic efficiency with formate as it enhances the kcat and kcat/Km values 3.48fold and 1.6fold, respectively, compared to the wild type enzyme | [Candida] boidinii |
V120S/N187D | the mutant has the highest catalytic efficiency with NAD+ as it displays an approximately 1.5fold increase in kcat/Km compared to the wild type enzyme | [Candida] boidinii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.02 | - |
NAD+ | mutant enzyme V120S/N187D, at pH 9.5 and 30°C | [Candida] boidinii | |
2.5 | - |
formate | mutant enzyme V120S/N187D, at pH 9.5 and 30°C | [Candida] boidinii | |
3.4 | - |
NAD+ | wild type enzyme, at pH 9.5 and 30°C | [Candida] boidinii | |
3.6 | - |
formate | wild type enzyme, at pH 9.5 and 30°C | [Candida] boidinii | |
3.8 | - |
NAD+ | mutant enzyme N187D, at pH 9.5 and 30°C | [Candida] boidinii | |
6.7 | - |
formate | mutant enzyme N187D, at pH 9.5 and 30°C | [Candida] boidinii | |
7.9 | - |
formate | mutant enzyme V120S, at pH 9.5 and 30°C | [Candida] boidinii | |
8.8 | - |
NAD+ | mutant enzyme V120S, at pH 9.5 and 30°C | [Candida] boidinii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
formate + NAD+ | [Candida] boidinii | - |
CO2 + NADH + H+ | - |
? | |
formate + NAD+ | [Candida] boidinii ATCC 32195 | - |
CO2 + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
[Candida] boidinii | O13437 | - |
- |
[Candida] boidinii ATCC 32195 | O13437 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
[Candida] boidinii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formate + NAD+ | - |
[Candida] boidinii | CO2 + NADH + H+ | - |
? | |
formate + NAD+ | - |
[Candida] boidinii ATCC 32195 | CO2 + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FDH | - |
[Candida] boidinii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
[Candida] boidinii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 40 | the enzyme remains stable after 1 h at 30-40°C | [Candida] boidinii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
formate | mutant enzyme V120S/N187D, at pH 9.5 and 30°C | [Candida] boidinii | |
1.53 | - |
formate | wild type enzyme, at pH 9.5 and 30°C | [Candida] boidinii | |
3.29 | - |
formate | mutant enzyme N187D, at pH 9.5 and 30°C | [Candida] boidinii | |
5.34 | - |
formate | mutant enzyme V120S, at pH 9.5 and 30°C | [Candida] boidinii | |
18.68 | - |
NAD+ | mutant enzyme V120S/N187D, at pH 9.5 and 30°C | [Candida] boidinii | |
22.3 | - |
NAD+ | wild type enzyme, at pH 9.5 and 30°C | [Candida] boidinii | |
28.99 | - |
NAD+ | mutant enzyme N187D, at pH 9.5 and 30°C | [Candida] boidinii | |
40.63 | - |
NAD+ | mutant enzyme V120S, at pH 9.5 and 30°C | [Candida] boidinii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
[Candida] boidinii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
[Candida] boidinii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.275 | - |
formate | mutant enzyme V120S/N187D, at pH 9.5 and 30°C | [Candida] boidinii | |
0.423 | - |
formate | wild type enzyme, at pH 9.5 and 30°C | [Candida] boidinii | |
0.491 | - |
formate | mutant enzyme N187D, at pH 9.5 and 30°C | [Candida] boidinii | |
0.676 | - |
formate | mutant enzyme V120S, at pH 9.5 and 30°C | [Candida] boidinii | |
5.008 | - |
NAD+ | mutant enzyme V120S, at pH 9.5 and 30°C | [Candida] boidinii | |
6.618 | - |
NAD+ | wild type enzyme, at pH 9.5 and 30°C | [Candida] boidinii | |
9.808 | - |
NAD+ | mutant enzyme N187D, at pH 9.5 and 30°C | [Candida] boidinii | |
9.934 | - |
NAD+ | mutant enzyme V120S/N187D, at pH 9.5 and 30°C | [Candida] boidinii |