EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
---|
1.5.1.3 | 10 |
- |
7,8-dihydrofolate |
deletion mutant DELTAArg52/DELTAGly121, at pH 7.0 and 25°C |
724416 |
1.5.1.3 | 20 |
- |
7,8-dihydrofolate |
mutant enzyme F96I, at pH 7.0 and 25°C |
725661 |
1.5.1.3 | 30 |
- |
7,8-dihydrofolate |
deletion mutant DELTAGly121/DELTAAla145, at pH 7.0 and 25°C |
724416 |
1.5.1.3 | 70 |
- |
7,8-dihydrofolate |
deletion mutant DELTAGly67/DELTAGly121, at pH 7.0 and 25°C |
724416 |
1.5.1.3 | 100 |
- |
7,8-dihydrofolate |
mutant enzyme R44P, at 15°C, pH not specified in the publication |
724168 |
1.5.1.3 | 120 |
- |
7,8-dihydrofolate |
mutant enzyme T86N, in 50 mM TES, pH 7.0, at 37°C |
723808 |
1.5.1.3 | 120 |
- |
7,8-dihydrofolate |
mutant enzyme Y102F, at pH 7.0 and 25°C |
725661 |
1.5.1.3 | 130 |
- |
7,8-dihydrofolate |
chimera with Homo sapiens enzyme, pH 7, 25°C |
765726 |
1.5.1.3 | 160 |
- |
7,8-dihydrofolate |
deletion mutant DELTAGly121, at pH 7.0 and 25°C |
724416 |
1.5.1.3 | 200 |
- |
7,8-dihydrofolate |
pH 8.0, 25°C |
764647 |