Protein Variants | Comment | Organism |
---|---|---|
additional information | replacement of an integral alpha-helical segment of Escherichia coli DHFR with segments from a number of different organisms (Listeria grayi, Haemophilus influenzae, Streptococcus dysgalactiae, Saccharomyces cerevisiae, Plasmodium falciparum, Homo sapiens). The parameters most sensitive to helical replacement are KM and Ki. The rank orders of KM, Ki, change of Tm, and protein stability against unfolding to the molten-globule state are significantly correlated with helical content | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0004 | - |
7,8-dihydrofolate | chimera with Haemophilus influenzae enzyme, pH 7, 25°C | Escherichia coli | |
0.0007 | - |
7,8-dihydrofolate | chimera with Listeria grayi enzyme, pH 7, 25°C | Escherichia coli | |
0.0008 | - |
7,8-dihydrofolate | wild-type, pH 7, 25°C | Escherichia coli | |
0.0015 | - |
7,8-dihydrofolate | chimera with Saccharomyces cerevisiae enzyme, pH 7, 25°C | Escherichia coli | |
0.0063 | - |
7,8-dihydrofolate | chimera with Streptococcus dysgalactiae enzyme, pH 7, 25°C | Escherichia coli | |
0.058 | - |
7,8-dihydrofolate | chimera with Plasmodium falciparum enzyme, pH 7, 25°C | Escherichia coli | |
0.067 | - |
7,8-dihydrofolate | chimera with Homo sapiens enzyme, pH 7, 25°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0ABQ4 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydrofolate + NADPH + H+ | - |
Escherichia coli | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9 | - |
7,8-dihydrofolate | chimera with Homo sapiens enzyme, pH 7, 25°C | Escherichia coli | |
10 | - |
7,8-dihydrofolate | chimera with Haemophilus influenzae enzyme, pH 7, 25°C | Escherichia coli | |
13 | - |
7,8-dihydrofolate | wild-type, pH 7, 25°C | Escherichia coli | |
14 | - |
7,8-dihydrofolate | chimera with Listeria grayi enzyme, pH 7, 25°C | Escherichia coli | |
29 | - |
7,8-dihydrofolate | chimera with Saccharomyces cerevisiae enzyme, pH 7, 25°C | Escherichia coli | |
33 | - |
7,8-dihydrofolate | chimera with Plasmodium falciparum enzyme, pH 7, 25°C | Escherichia coli | |
37 | - |
7,8-dihydrofolate | chimera with Streptococcus dysgalactiae enzyme, pH 7, 25°C | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
130 | - |
7,8-dihydrofolate | chimera with Homo sapiens enzyme, pH 7, 25°C | Escherichia coli | |
500 | - |
7,8-dihydrofolate | chimera with Plasmodium falciparum enzyme, pH 7, 25°C | Escherichia coli | |
6000 | - |
7,8-dihydrofolate | chimera with Streptococcus dysgalactiae enzyme, pH 7, 25°C | Escherichia coli | |
16000 | - |
7,8-dihydrofolate | wild-type, pH 7, 25°C | Escherichia coli | |
22000 | - |
7,8-dihydrofolate | chimera with Listeria grayi enzyme, pH 7, 25°C | Escherichia coli | |
24000 | - |
7,8-dihydrofolate | chimera with Saccharomyces cerevisiae enzyme, pH 7, 25°C | Escherichia coli | |
25000 | - |
7,8-dihydrofolate | chimera with Haemophilus influenzae enzyme, pH 7, 25°C | Escherichia coli |