EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
---|
1.3.1.9 | 0.011 |
- |
(2E)-2-dodecenoyl-CoA |
mutant K245M, pH 7.9, 25°C |
711222 |
1.3.1.9 | 0.025 |
- |
(2E)-2-dodecenoyl-CoA |
mutant K244A, pH 7.9, 25°C |
711222 |
1.3.1.9 | 0.028 |
- |
(2E)-2-dodecenoyl-CoA |
mutant K244R, pH 7.9, 25°C |
711222 |
1.3.1.9 | 0.03 |
- |
(2E)-2-dodecenoyl-CoA |
mutant Y235A, pH 7.9, 25°C |
711222 |
1.3.1.9 | 0.047 |
- |
(2E)-2-dodecenoyl-CoA |
mutant Y235S, pH 7.9, 25°C |
711222 |
1.3.1.9 | 1.15 |
- |
crotonyl-CoA |
at pH 7.5 and 30°C |
739906 |
1.3.1.9 | 5 |
- |
trans-2-dodecenoyl-CoA |
mutant enzyme T267Y, at pH 8.0 and 30°C |
740080 |
1.3.1.9 | 5 |
- |
trans-2-dodecenoyl-CoA |
pH 6.8, 25°C, mutant enzyme Y158F |
747054 |
1.3.1.9 | 6.5 |
- |
(2E)-2-dodecenoyl-CoA |
wild-type, pH 7.9, 25°C |
711222 |
1.3.1.9 | 8.3 |
- |
trans-2-dodecenoyl-CoA |
mutant P193A, pH 7.5, 37°C |
763718 |