Protein Variants | Comment | Organism |
---|---|---|
M155A | vmax for NADH is similar to that of wild-type, the M155A Vmax for trans-2-dodecenoyl-CoA is around 64% of the wild-type rate | Mycobacterium tuberculosis |
P193A | mutation inactivates the ability of InhA to turn over either NADH or trans-2-dodecenoyl-CoA | Mycobacterium tuberculosis |
S94A | similar activity compared to wild-type but a reduced NADH affinity, the affinity of S94A for is also decreased | Mycobacterium tuberculosis |
W222A | reduction in overall enoyl reductase activity, mutant shows an increased affinity for NADH | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
NADH | mutant P193A, pH 7.5, 37°C | Mycobacterium tuberculosis | |
0.026 | - |
trans-2-dodecenoyl-CoA | mutant M155A, pH 7.5, 37°C | Mycobacterium tuberculosis | |
0.053 | - |
trans-2-dodecenoyl-CoA | mutant P193A, pH 7.5, 37°C | Mycobacterium tuberculosis | |
0.065 | - |
NADH | mutant M155A, pH 7.5, 37°C | Mycobacterium tuberculosis | |
0.075 | - |
trans-2-dodecenoyl-CoA | wild-type, pH 7.5, 37°C | Mycobacterium tuberculosis | |
0.083 | - |
NADH | mutant W222A, pH 7.5, 37°C | Mycobacterium tuberculosis | |
0.098 | - |
NADH | wild-type, pH 7.5, 37°C | Mycobacterium tuberculosis | |
0.106 | - |
trans-2-dodecenoyl-CoA | mutant S94A, pH 7.5, 37°C | Mycobacterium tuberculosis | |
0.145 | - |
trans-2-dodecenoyl-CoA | mutant W222A, pH 7.5, 37°C | Mycobacterium tuberculosis | |
0.211 | - |
NADH | mutant S94A, pH 7.5, 37°C | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
trans-2-dodecenoyl-CoA + NADH + H+ | - |
Mycobacterium tuberculosis | dodecanoyl-CoA + NAD+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
InhA | - |
Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
NADH | mutant P193A, pH 7.5, 37°C | Mycobacterium tuberculosis | |
3.8 | - |
trans-2-dodecenoyl-CoA | mutant P193A, pH 7.5, 37°C | Mycobacterium tuberculosis | |
7.4 | - |
trans-2-dodecenoyl-CoA | mutant M155A, pH 7.5, 37°C | Mycobacterium tuberculosis | |
10.3 | - |
NADH | mutant W222A, pH 7.5, 37°C | Mycobacterium tuberculosis | |
11.6 | - |
trans-2-dodecenoyl-CoA | wild-type, pH 7.5, 37°C | Mycobacterium tuberculosis | |
13.2 | - |
trans-2-dodecenoyl-CoA | mutant S94A, pH 7.5, 37°C | Mycobacterium tuberculosis | |
17 | - |
NADH | wild-type, pH 7.5, 37°C | Mycobacterium tuberculosis | |
17.6 | - |
trans-2-dodecenoyl-CoA | mutant W222A, pH 7.5, 37°C | Mycobacterium tuberculosis | |
17.9 | - |
NADH | mutant M155A, pH 7.5, 37°C | Mycobacterium tuberculosis | |
29.1 | - |
NADH | mutant S94A, pH 7.5, 37°C | Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.3 | - |
trans-2-dodecenoyl-CoA | mutant P193A, pH 7.5, 37°C | Mycobacterium tuberculosis | |
20 | - |
NADH | mutant P193A, pH 7.5, 37°C | Mycobacterium tuberculosis | |
122 | - |
trans-2-dodecenoyl-CoA | mutant W222A, pH 7.5, 37°C | Mycobacterium tuberculosis | |
123 | - |
trans-2-dodecenoyl-CoA | mutant S94A, pH 7.5, 37°C | Mycobacterium tuberculosis | |
125 | - |
NADH | mutant W222A, pH 7.5, 37°C | Mycobacterium tuberculosis | |
142 | - |
NADH | mutant S94A, pH 7.5, 37°C | Mycobacterium tuberculosis | |
155 | - |
trans-2-dodecenoyl-CoA | wild-type, pH 7.5, 37°C | Mycobacterium tuberculosis | |
173 | - |
NADH | wild-type, pH 7.5, 37°C | Mycobacterium tuberculosis | |
277 | - |
NADH | mutant M155A, pH 7.5, 37°C | Mycobacterium tuberculosis | |
283 | - |
trans-2-dodecenoyl-CoA | mutant M155A, pH 7.5, 37°C | Mycobacterium tuberculosis |