EC Number   |
General Stability |
Reference |
|---|
    1.17.1.9 | after modification with either cholinium, hydroxyethyl-methylimidazolium, or hydroxypropyl-methylimidazolium cations, the FDH still presents 30-45% of activity in aqueous buffer in 70% (v/v) dimethylimidazolium dimethyl phosphate while the native enzyme is inactive at this ionic liquid concentration. The half-life of the modified enzyme is also increased by a 5fold factor after modification by cholinium (9 days) and by a 3fold factor after modification by hydroxyethyl-methylimidazolium or hydroxypropyl-methylimidazolium cations (6 and 5 days respectively) in aqueous solution. The half-life of the wild type FDH in aqueous solution is 1.5 days. When stored in 37.5% (v/v) dimethylimidazolium dimethyl phosphate, both modified and unmodified FDH have an increased half-life of 6-9 days |
712790 |
| 1.17.1.9 | both dithiothreitol, 10 mM, and glycerol, 10%, are required for stability during purification |
288110 |
| 1.17.1.9 | enzyme immobilized on highly activated glyoxyl agarose, retains full activity after 24 h in 50% acetone or dioxane. At 75% acetone, immobilized enzyme keeps more than 65% of initial activity after 4 days |
672513 |
| 1.17.1.9 | formate dehydrogenase immobilized on magnetic glyoxylagarose support suffers a decrease in formate conversion with the increase in immobilization time. MagNP-APTS/FDH (enzyme on a magnetic support containing a silica gel coated magnetite core which is further silanized with a minopropyltriethoxysilane minopropyltriethoxysilane) shows increased stability when incubated for up to 1 h at 60°C and pH 7.5, while MagNP-APTS/glyoxyl-agarose/FDH (enzyme on a magnetic support consisting of magnetite-aminopropyltriethoxysilane coatedwith glyoxyl-agarose) is completely inactive after 20 min of incubation |
725739 |
| 1.17.1.9 | inactivated by light irradiation |
288087 |
| 1.17.1.9 | instability to freezing and thawing |
288094 |
| 1.17.1.9 | N3- and NO3- stabilize |
288123 |
| 1.17.1.9 | rapid freezing in liquid nitrogen gives 75% recovery |
80983 |
| 1.17.1.9 | strong inhibition by light |
288094 |
| 1.17.1.9 | the activities of liposomal FDH/cofactor systems are highly stable in the airlift regardless of the type of gas phase, whereas free FDH is deactivated in the airlift especially at high superficial gas velocity with CO2 bubbles (after 360 min at 45°C) |
711548 |
