Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Aprotinin | - |
Bos taurus | |
Bovine pancreatic trypsin inhibitor | pH-dependent | Bos taurus | |
diisopropyl fluorophosphate | - |
Bos taurus | |
leupeptin | - |
Bos taurus | |
N-tosyl-L-Lys chloromethyl ketone | - |
Bos taurus | |
N-tosyl-L-Phe chloromethyl ketone | weak | Bos taurus | |
NaCl | - |
Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0002 | - |
Benzyloxycarbonyl-Ala-Arg-Arg 4-methylcoumarin 7-amide | - |
Bos taurus | |
0.0046 | - |
Tert-Butyloxycarbonyl-Gly-Lys-Arg 4-methylcoumarin 7-amide | - |
Bos taurus | |
0.0231 | - |
benzyloxycarbonyl-Arg 4-methylcoumarin 7-amide | - |
Bos taurus | |
0.029 | - |
tert-butyloxycarbonyl-Phe-Ser-Arg 4-methylcoumarin 7-amide | - |
Bos taurus | |
0.031 | - |
tert-butyloxycarbonyl-Val-Pro-Arg 4-methylcoumarin 7-amide | - |
Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
x * 39000 + x * 40000, SDS-PAGE of DFP-labelled enzyme | Bos taurus |
40000 | - |
x * 39000 + x * 40000, SDS-PAGE of DFP-labelled enzyme | Bos taurus |
360000 | - |
gel filtration | Bos taurus |
360000 | - |
bovine | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mast cell | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Bos taurus |
Storage Stability | Organism |
---|---|
-20°C, high salt concentration, 50% glycerol, stable for some months | Bos taurus |
4°C, pH 6.1, high salt concentration, stable for about 2 or 3 weeks | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Benzyloxycarbonyl-Ala-Arg-Arg 4-methylcoumarin 7-amide + H2O | - |
Bos taurus | Benzyloxycarbonyl-Ala-Arg-Arg + 7-amino-4-methylcoumarin | - |
? | |
Benzyloxycarbonyl-Arg 4-methylcoumarin 7-amide + H2O | - |
Bos taurus | Benzyloxycarbonyl-Arg + 7-amino-4-methylcoumarine | - |
? | |
N-tert-Butyloxycarbonyl-Phe-Ser-Arg 4-methylcoumarin 7-amide + H2O | - |
Bos taurus | N-tert-Butyloxycarbonyl-Phe-Ser-Arg + 7-amino-4-methylcoumarin | - |
? | |
N-tert-Butyloxycarbonyl-Val-Pro-Arg 4-methylcoumarin 7-amide + H2O | - |
Bos taurus | N-tert-Butyloxycarbonyl-Val-Pro-Arg + 7-amino-4-methylcoumarin | - |
? | |
tert-butyloxycarbonyl-Gly-Lys-Arg 4-methylcoumarin 7-amide + H2O | - |
Bos taurus | tert-butyloxycarbonyl-Gly-Lys-Arg + 7-amino-4-methylcoumarin | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 39000 + x * 40000, SDS-PAGE of DFP-labelled enzyme | Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Bos taurus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
benzyloxycarbonyl-Arg 4-methylcoumarin 7-amide | - |
Bos taurus | |
0.5 | - |
Tert-Butyloxycarbonyl-Gly-Lys-Arg 4-methylcoumarin 7-amide | - |
Bos taurus | |
0.8 | - |
Benzyloxycarbonyl-Ala-Arg-Arg 4-methylcoumarin 7-amide | - |
Bos taurus | |
1.4 | - |
tert-butyloxycarbonyl-L-Val-L-Pro-L-Arg-4-methylcoumarin 7-amide | - |
Bos taurus | |
2.2 | - |
tert-butyloxycarbonyl-Phe-Ser-Arg 4-methylcoumarin 7-amide | - |
Bos taurus |