Inhibitors | Comment | Organism | Structure |
---|---|---|---|
antipain | - |
Homo sapiens | |
CaCl2 | - |
Homo sapiens | |
D-Tyr-Glu-Phe-Lys-Arg-CH2Cl | - |
Homo sapiens | |
diisopropyl fluorophosphate | - |
Homo sapiens | |
DTNB | - |
Homo sapiens | |
HgCl2 | - |
Homo sapiens | |
leupeptin | - |
Homo sapiens | |
N-tosyl-L-Lys chloromethyl ketone | - |
Homo sapiens | |
NaCl | reversible at 1 M | Homo sapiens | |
p-chloromercuribenzoate | - |
Homo sapiens | |
PMSF | - |
Homo sapiens | |
ZnSO4 | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0138 | - |
Benzyloxycarbonyl-L-Ala-L-Lys-L-Arg 4-methylcoumarin 7-amide | - |
Homo sapiens | |
0.0148 | - |
Benzyloxycarbonyl-L-Ala-L-Lys-L-Lys 4-methylcoumarin 7-amide | - |
Homo sapiens | |
0.0409 | - |
Benzyloxycarbonyl-L-Ala-L-Arg-L-Arg 4-methylcoumarin 7-amide | - |
Homo sapiens | |
0.323 | - |
benzyloxycarbonyl-L-Ala-L-Leu-L-Lys-7-amido-4-methylcoumarin | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
110000 | - |
human, gel filtration in presence of 0.3 M NaCl | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | some of the MW heterogeneity of the multiple forms results from N-linked glycosylation | Homo sapiens |
Purification (Comment) | Organism |
---|---|
multiple forms | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mast cell | present in pituitary connective tissue | Homo sapiens | - |
Storage Stability | Organism |
---|---|
-20°C | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Benzyloxycarbonyl-L-Ala-L-Arg-L-Arg 4-methylcoumarin 7-amide + H2O | - |
Homo sapiens | Benzyloxycarbonyl-L-Ala-L-Arg-L-Arg + 7-amino-4-methylcoumarin | - |
? | |
benzyloxycarbonyl-L-Ala-L-Leu-L-Lys-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | benzyloxycarbonyl-L-Ala-L-Leu-L-Lys + 7-amino-4-methylcoumarin | - |
? | |
Benzyloxycarbonyl-L-Ala-L-Lys-L-Arg 4-methylcoumarin 7-amide + H2O | - |
Homo sapiens | Benzyloxycarbonyl-L-Ala-L-Lys-L-Arg + 7-amino-4-methylcoumarin | - |
? | |
Benzyloxycarbonyl-L-Ala-L-Lys-L-Lys 4-methylcoumarin 7-amide + H2O | - |
Homo sapiens | Benzyloxycarbonyl-L-Ala-L-Lys-L-Lys + 7-amino-4-methylcoumarin | - |
? | |
additional information | pituitary enzyme has a preference for cleaving COOH-terminal to arginine or lysine residues which are preceded by a proline residue 4 or 6 residues NH2-terminal to the site of cleavage | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 32400-36300, human, SDS-PAGE, various enzyme forms | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1520 | - |
Benzyloxycarbonyl-L-Ala-L-Lys-L-Lys 4-methylcoumarin 7-amide | - |
Homo sapiens | |
1870 | - |
benzyloxycarbonyl-L-Ala-L-Leu-L-Lys-7-amido-4-methylcoumarin | - |
Homo sapiens | |
2040 | - |
Benzyloxycarbonyl-L-Ala-L-Arg-L-Arg 4-methylcoumarin 7-amide | - |
Homo sapiens | |
3290 | - |
Benzyloxycarbonyl-L-Ala-L-Lys-L-Arg 4-methylcoumarin 7-amide | - |
Homo sapiens |