General Stability | Organism |
---|---|
addition of protein to the tryptase-heparin mixture is needed to keep the enzyme stable | Homo sapiens |
heparin glycosaminoglycan partially stabilizes the tryptase | Homo sapiens |
In low-salt buffer the enzyme is labile, at least 1.4 M KCl is needed to keep the enzyme stable if incubated at 37°C for 30 min | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
benzamidine | - |
Homo sapiens | |
diisopropyl fluorophosphate | - |
Homo sapiens | |
leupeptin | - |
Homo sapiens | |
N-tosyl-L-Phe chloromethyl ketone | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
x * 34000 + x * 38000, human, SDS-PAGE | Homo sapiens |
38000 | - |
x * 34000 + x * 38000, human, SDS-PAGE | Homo sapiens |
120000 | - |
human, gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
lung | - |
Homo sapiens | - |
skin | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Benzyloxycarbonyl-Arg 4-nitroanilide + H2O | - |
Homo sapiens | Benzyloxycarbonyl-Arg + 4-nitroaniline | - |
? | |
Benzyloxycarbonyl-Gly-Arg 4-trifluoromethylcoumarin 7-amide + H2O | - |
Homo sapiens | Benzyloxycarbonyl-Gly-Arg + 7-amino-4-trifluoromethylcoumarin | - |
? | |
Benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide + H2O | - |
Homo sapiens | Benzyloxycarbonyl-Gly-Pro-Arg + 4-nitroaniline | - |
? | |
Benzyloxycarbonyl-Lys-Arg 4-trifluoromethylcoumarin 7-amide + H2O | - |
Homo sapiens | Benzyloxycarbonyl-Lys-Arg + 7-amino-4-trifluoromethylcoumarin | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | x * 34000 + x * 38000, human, SDS-PAGE | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.35 | - |
benzyloxycarbonyl-Arg 4-nitroanilide | skin enzyme | Homo sapiens | |
5.52 | - |
benzyloxycarbonyl-Arg 4-nitroanilide | lung enzyme | Homo sapiens | |
45.7 | - |
Benzyloxycarbonyl-Lys-Arg 4-trifluoromethylcoumarin 7-amide | skin and lung enzyme | Homo sapiens | |
104 | - |
Benzyloxycarbonyl-Gly-Arg 4-trifluoromethylcoumarin 7-amide | skin enzyme | Homo sapiens | |
107 | - |
Benzyloxycarbonyl-Gly-Arg 4-trifluoromethylcoumarin 7-amide | lung enzyme | Homo sapiens | |
551 | - |
Benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide | skin enzyme | Homo sapiens | |
553 | - |
Benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide | lung enzyme | Homo sapiens |