Cloned (Comment) | Organism |
---|---|
bioF gene overexpression in Escherichia coli | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
pH 8.5-8.9, ammonium sulfate precipitation, apoenzyme, X-ray diffraction structure analysis with and without bound pyridoxyl-5'-phosphate, model, active site residues | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
amino acid sequence | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-carboxyhexanoyl-CoA + L-alanine | Escherichia coli | i.e. pimeloyl-CoA | 8-amino-7-oxononanoate + CoA + CO2 | i.e. 7-oxo-8-amino-pelargonic acid | ? | |
6-carboxyhexanoyl-CoA + L-alanine | Escherichia coli | reaction in biotin biosynthesis | 8-amino-7-oxononanoate + CoA + CO2 | i.e. 7-oxo-8-amino-pelargonic acid | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P12998 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant from E. coli | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
pimeloyl-[acyl-carrier protein] + L-alanine = 8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein] | reaction mechanism | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-carboxyhexanoyl-CoA + L-alanine | i.e. pimeloyl-CoA | Escherichia coli | 8-amino-7-oxononanoate + CoA + CO2 | i.e. 7-oxo-8-amino-pelargonic acid | ? | |
6-carboxyhexanoyl-CoA + L-alanine | reaction in biotin biosynthesis | Escherichia coli | 8-amino-7-oxononanoate + CoA + CO2 | i.e. 7-oxo-8-amino-pelargonic acid | ? |
Subunits | Comment | Organism |
---|---|---|
More | dimer, active site is formed at the interface between the monomers | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
AONS | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Escherichia coli |