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(R)-8-amino-7-oxononanoic acid + S-adenosyl-L-methionine
(7R,8S)-diaminopelargonic acid + S-adenosyl-(4-methylthio-2-oxobutanoic acid)
-
8.6% product is formed matching the portion of S-enantiomer substrate (9.3%),37°C, direct assay with 100 mM N-(2-hydroxyethyl)piperazine-N'-(2-propanesulfonic acid) buffer (pH 8.6), 0.1 mM pyridoxal 5'-phosphate, 1 mM S-adenosyl-L-methionine, 10 microM (R)-8-amino-7-oxononanoic acid
-
-
?
(S)-8-amino-7-oxononanoic acid + (R)-8-amino-7-oxononanoic acid + S-adenosyl-L-methionine
(7R,8S)-diaminopelargonic acid + S-adenosyl-(4-methylthio-2-oxobutanoic acid)
-
46.3% product is formed from the racemic substrate, 37°C, direct assay with 100 mM N-(2-hydroxyethyl)piperazine-N'-(2-propanesulfonic acid) buffer (pH 8.6), 0.1 mM pyridoxal 5'-phosphate, 1 mM S-adenosyl-L-methionine, 2 microM racemic 8-amino-7-oxononanoic acid
-
-
?
(S)-8-amino-7-oxononanoic acid + S-adenosyl-L-methionine
(7R,8S)-diaminopelargonic acid + S-adenosyl-(4-methylthio-2-oxobutanoic acid)
6-carboxyhexanoyl-CoA + D-alanine
8-amino-7-oxononanoate + CoA + CO2
-
the L-isomer of the amino acid is preferred, D-alanine shows 5fold slower reaction, interaction analysis, overview
i.e. D-KAPA
-
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
6-carboxyhexanoyl-CoA + L-serine
9-hydroxy-8-amino-7-oxononanoate + CoA
-
low activity
-
?
acetyl-CoA + L-arginine
N-[(4S)-4-amino-5-oxohexyl]guanidine + CO2 + CoA
benzoyl-CoA + L-arginine
N-[(4S)-4-amino-5-oxo-5-phenylpentyl]guanidine + CO2 + CoA
butanoyl-CoA + L-arginine
N-[(4S)-4-amino-5-oxooctyl]guanidine + CO2 + CoA
cyclopentanoyl-CoA + L-arginine
N-[(4S)-4-amino-5-cyclopentyl-5-oxopentyl]guanidine + CO2 + CoA
hexanoyl-CoA + L-arginine
N-[(4S)-4-amino-5-oxodecyl]guanidine + CO2 + CoA
indole-3-carboxylic acid + 1-(2,6-dihydroxyphenyl)-butan-1-one
indolchromin A + CO2 + H2O
-
-
-
-
?
isobutanoyl-CoA + L-arginine
N-[(4S)-4-amino-6-methyl-5-oxoheptyl]guanidine + CO2 + CoA
-
-
-
-
?
isovaleryl-CoA + L-arginine
N-[(4S)-4-amino-7-methyl-5-oxooctyl]guanidine + CO2 + CoA
-
-
-
-
?
L-alanine + pimeloyl-CoA
8-amino-7-oxononanoate + CoA + CO2
octanoyl-CoA + L-arginine
N-[(4S)-4-amino-5-oxododecyl]guanidine + CO2 + CoA
-
-
-
-
?
pimeloyl-CoA + D-alanine
?
-
-
-
-
?
pimeloyl-CoA + L-alanine
8-amino-7-oxononanoate + CO2 + CoA
pimeloyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
pimeloyl-CoA + L-alanine
?
-
-
-
-
?
pimeloyl-CoA + L-alanine methyl ester
?
-
trapping and determination of the reaction intermediate using the the more stable methyl ester of the substrate, overview
-
-
?
pimeloyl-[acyl carrier protein] + L-alanine
8-amino-7-oxononanoate + CO2 + [acyl carrier protein]
-
-
-
?
pimeloyl-[acyl-carrier protein] + L-alanine
8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein]
propionyl-CoA + L-arginine
N-[(4S)-4-amino-5-oxoheptyl]guanidine + CO2 + CoA
-
-
-
-
?
propionyl-[acyl-carrier protein] + L-alanine
1-[(4S)-4-amino-5-oxoheptyl]guanidine + CO2 + holo-[acyl-carrier protein]
-
-
-
-
?
propionyl-[acyl-carrier protein] + L-arginine
N-[(4S)-4-amino-5-oxoheptyl]guanidine + CO2 + holo-[acyl-carrier protein]
-
-
-
-
?
additional information
?
-
(S)-8-amino-7-oxononanoic acid + S-adenosyl-L-methionine
(7R,8S)-diaminopelargonic acid + S-adenosyl-(4-methylthio-2-oxobutanoic acid)
-
37°C, direct assay with 100 mM N-(2-hydroxyethyl)piperazine-N'-(2-propanesulfonic acid) buffer (pH 8.6), 0.1 mM pyridoxal 5'-phosphate, varying S-adenosyl-L-methionine, 9 microM (S)-8-amino-7-oxononanoic acid, varying inhibitor concentration
-
-
?
(S)-8-amino-7-oxononanoic acid + S-adenosyl-L-methionine
(7R,8S)-diaminopelargonic acid + S-adenosyl-(4-methylthio-2-oxobutanoic acid)
-
37°C, direct assay with 100 mM N-(2-hydroxyethyl)piperazine-N'-(2-propanesulfonic acid) buffer (pH 8.6), 0.1 mM pyridoxal 5'-phosphate, varying S-adenosyl-L-methionine, 9 microM (S)-8-amino-7-oxononanoic acid, varying inhibitor concentration
-
-
?
(S)-8-amino-7-oxononanoic acid + S-adenosyl-L-methionine
(7R,8S)-diaminopelargonic acid + S-adenosyl-(4-methylthio-2-oxobutanoic acid)
-
37°C, direct assay with 100 mM N-(2-hydroxyethyl)piperazine-N'-(2-propanesulfonic acid) buffer (pH 8.6), 0.1 mM pyridoxal 5'-phosphate, 1 mM S-adenosyl-L-methionine, 2 microM (S)-8-amino-7-oxononanoic acid
-
-
?
(S)-8-amino-7-oxononanoic acid + S-adenosyl-L-methionine
(7R,8S)-diaminopelargonic acid + S-adenosyl-(4-methylthio-2-oxobutanoic acid)
-
93.2% product is formed matching the portion of S-enantiomer substrate (94.6%), 37°C, direct assay with 100 mM N-(2-hydroxyethyl)piperazine-N'-(2-propanesulfonic acid) buffer (pH 8.6), 0.1 mM pyridoxal 5'-phosphate, 1 mM S-adenosyl-L-methionine, 2 microM (S)-8-amino-7-oxononanoic acid
-
-
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
i.e. pimeloyl-CoA
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
reaction in biotin biosynthesis
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
i.e. pimeloyl-CoA
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
reaction in biotin biosynthesis
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
i.e. pimeloyl-CoA
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
reaction in biotin biosynthesis
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
i.e. pimeloyl-CoA
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
reaction in biotin biosynthesis
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
Erwinia aroidea
-
i.e. pimeloyl-CoA
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
Erwinia aroidea
-
reaction in biotin biosynthesis
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
-
-
-
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
i.e. pimeloyl-CoA
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
i.e. pimeloyl-CoA
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
i.e. pimeloyl-CoA
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
i.e. pimeloyl-CoA
i.e. 7-oxo-8-amino-pelargonic acid
ir
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
i.e. pimeloyl-CoA
i.e. 7-oxo-8-amino-pelargonic acid
ir
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
i.e. pimeloyl-CoA
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
reaction in biotin biosynthesis
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
reaction in biotin biosynthesis
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
reaction in biotin biosynthesis
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
reaction in biotin biosynthesis
i.e. 7-oxo-8-amino-pelargonic acid
ir
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
reaction in biotin biosynthesis
i.e. 7-oxo-8-amino-pelargonic acid
ir
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
reaction in biotin biosynthesis
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
the enzyme catalyzes the first committed step in the four step pathway, the decarboxylative condensation of L-alanine and pimeloyl-CoA to give (7S)-8-amino-7-oxononanoate
-
-
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
a Claisen condensation
-
-
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
i.e. pimeloyl-CoA
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
reaction in biotin biosynthesis
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
i.e. pimeloyl-CoA
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
L-cysteine cannot replace L-alanine
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
reaction in biotin biosynthesis
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
i.e. pimeloyl-CoA
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
reaction in biotin biosynthesis
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
absolutely specific for L-alanine
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
i.e. pimeloyl-CoA
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
i.e. pimeloyl-CoA
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
i.e. pimeloyl-CoA
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
i.e. pimeloyl-CoA
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
i.e. pimeloyl-CoA
i.e. 7-oxo-8-amino-pelargonic acid
ir
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
i.e. pimeloyl-CoA
i.e. 7-oxo-8-amino-pelargonic acid
ir
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
i.e. pimeloyl-CoA
i.e. 7-oxo-8-amino-pelargonic acid
ir
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
L-cysteine cannot replace L-alanine
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
second step in biotin biosynthesis
i.e. 7-oxo-8-amino-pelargonic acid
ir
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
second step in biotin biosynthesis
i.e. 7-oxo-8-amino-pelargonic acid
ir
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
reaction in biotin biosynthesis
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
reaction in biotin biosynthesis
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
reaction in biotin biosynthesis
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
reaction in biotin biosynthesis
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
reaction in biotin biosynthesis
i.e. 7-oxo-8-amino-pelargonic acid
ir
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
reaction in biotin biosynthesis
i.e. 7-oxo-8-amino-pelargonic acid
ir
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
reaction in biotin biosynthesis
i.e. 7-oxo-8-amino-pelargonic acid
ir
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
first committed step in the biotin biosynthesis pathway
-
-
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
the L-isomer of the amino acid is preferred giving a 5fold faster reaction, interaction analysis, overview
i.e. L-KAPA
-
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
i.e. pimeloyl-CoA
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
L-cysteine cannot replace L-alanine
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
reaction in biotin biosynthesis
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
i.e. pimeloyl-CoA
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
reaction in biotin biosynthesis
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
Sporobolomyces coprophilus
-
i.e. pimeloyl-CoA
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
Sporobolomyces coprophilus
-
reaction in biotin biosynthesis
i.e. 7-oxo-8-amino-pelargonic acid
?
acetyl-CoA + L-arginine
N-[(4S)-4-amino-5-oxohexyl]guanidine + CO2 + CoA
-
-
-
-
?
acetyl-CoA + L-arginine
N-[(4S)-4-amino-5-oxohexyl]guanidine + CO2 + CoA
-
-
-
-
?
benzoyl-CoA + L-arginine
N-[(4S)-4-amino-5-oxo-5-phenylpentyl]guanidine + CO2 + CoA
-
-
-
-
?
benzoyl-CoA + L-arginine
N-[(4S)-4-amino-5-oxo-5-phenylpentyl]guanidine + CO2 + CoA
-
-
-
-
?
butanoyl-CoA + L-arginine
N-[(4S)-4-amino-5-oxooctyl]guanidine + CO2 + CoA
-
-
-
-
?
butanoyl-CoA + L-arginine
N-[(4S)-4-amino-5-oxooctyl]guanidine + CO2 + CoA
-
-
-
-
?
cyclopentanoyl-CoA + L-arginine
N-[(4S)-4-amino-5-cyclopentyl-5-oxopentyl]guanidine + CO2 + CoA
-
-
-
-
?
cyclopentanoyl-CoA + L-arginine
N-[(4S)-4-amino-5-cyclopentyl-5-oxopentyl]guanidine + CO2 + CoA
-
-
-
-
?
glycine + acetyl-CoA
?
-
-
-
-
?
glycine + acetyl-CoA
?
-
-
-
-
?
glycine + pimeloyl-CoA
?
-
-
-
-
?
glycine + pimeloyl-CoA
?
-
-
-
-
?
hexanoyl-CoA + L-arginine
N-[(4S)-4-amino-5-oxodecyl]guanidine + CO2 + CoA
-
-
-
-
?
hexanoyl-CoA + L-arginine
N-[(4S)-4-amino-5-oxodecyl]guanidine + CO2 + CoA
-
-
-
-
?
L-alanine + acetyl-CoA
?
-
-
-
-
?
L-alanine + acetyl-CoA
?
-
-
-
-
?
L-alanine + pimeloyl-CoA
8-amino-7-oxononanoate + CoA + CO2
-
-
-
-
?
L-alanine + pimeloyl-CoA
8-amino-7-oxononanoate + CoA + CO2
-
-
-
-
?
L-alanine + pimeloyl-CoA
8-amino-7-oxononanoate + CoA + CO2
-
no activity observed with acetyl-CoA, no activity observed with glycine or L-serine
-
-
?
L-alanine + pimeloyl-CoA
8-amino-7-oxononanoate + CoA + CO2
-
-
-
-
?
L-alanine + pimeloyl-CoA
8-amino-7-oxononanoate + CoA + CO2
-
-
-
-
?
L-alanine + pimeloyl-CoA
8-amino-7-oxononanoate + CoA + CO2
-
-
-
?
L-alanine + pimeloyl-CoA
8-amino-7-oxononanoate + CoA + CO2
-
no activity observed with acetyl-CoA, no activity observed with glycine or L-serine
-
-
?
L-alanine + pimeloyl-CoA
8-amino-7-oxononanoate + CoA + CO2
-
no activity observed with acetyl-CoA, no activity observed with glycine or L-serine
-
-
?
L-alanine + pimeloyl-CoA
8-amino-7-oxononanoate + CoA + CO2
-
no activity observed with L-serine
-
-
?
L-alanine + pimeloyl-CoA
8-amino-7-oxononanoate + CoA + CO2
-
no activity observed with L-serine
-
-
?
L-alanine + pimeloyl-CoA
8-amino-7-oxononanoate + CoA + CO2
Thermosynechococcus vestitus
-
-
-
-
?
pimeloyl-CoA + L-alanine
8-amino-7-oxononanoate + CO2 + CoA
-
-
-
?
pimeloyl-CoA + L-alanine
8-amino-7-oxononanoate + CO2 + CoA
-
-
-
?
pimeloyl-CoA + L-alanine
8-amino-7-oxononanoate + CO2 + CoA
-
-
-
?
pimeloyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
-
-
?
pimeloyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
the enzyme is strictly stereospecific for L-alanine
-
-
?
pimeloyl-[acyl-carrier protein] + L-alanine
8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein]
-
-
-
?
pimeloyl-[acyl-carrier protein] + L-alanine
8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein]
-
-
-
?
pimeloyl-[acyl-carrier protein] + L-alanine
8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein]
-
-
-
-
?
pimeloyl-[acyl-carrier protein] + L-alanine
8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein]
-
-
-
?
additional information
?
-
the enzyme catalyzes the first committed enzyme of the biotin synthesis pathway
-
-
?
additional information
?
-
the enzyme is unable to utilize pimeloyl-[acyl carrier protein]
-
-
-
additional information
?
-
-
the enzyme is unable to utilize pimeloyl-[acyl carrier protein]
-
-
-
additional information
?
-
the enzyme is unable to utilize pimeloyl-[acyl carrier protein]
-
-
-
additional information
?
-
-
pimeloyl-CoA forms a quinonoid with L- or D-KAPA
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(S)-8-amino-7-oxononanoic acid + S-adenosyl-L-methionine
(7R,8S)-diaminopelargonic acid + S-adenosyl-(4-methylthio-2-oxobutanoic acid)
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
pimeloyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
-
-
?
pimeloyl-[acyl-carrier protein] + L-alanine
8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein]
additional information
?
-
the enzyme catalyzes the first committed enzyme of the biotin synthesis pathway
-
-
?
(S)-8-amino-7-oxononanoic acid + S-adenosyl-L-methionine
(7R,8S)-diaminopelargonic acid + S-adenosyl-(4-methylthio-2-oxobutanoic acid)
-
37°C, direct assay with 100 mM N-(2-hydroxyethyl)piperazine-N'-(2-propanesulfonic acid) buffer (pH 8.6), 0.1 mM pyridoxal 5'-phosphate, varying S-adenosyl-L-methionine, 9 microM (S)-8-amino-7-oxononanoic acid, varying inhibitor concentration
-
-
?
(S)-8-amino-7-oxononanoic acid + S-adenosyl-L-methionine
(7R,8S)-diaminopelargonic acid + S-adenosyl-(4-methylthio-2-oxobutanoic acid)
-
37°C, direct assay with 100 mM N-(2-hydroxyethyl)piperazine-N'-(2-propanesulfonic acid) buffer (pH 8.6), 0.1 mM pyridoxal 5'-phosphate, varying S-adenosyl-L-methionine, 9 microM (S)-8-amino-7-oxononanoic acid, varying inhibitor concentration
-
-
?
(S)-8-amino-7-oxononanoic acid + S-adenosyl-L-methionine
(7R,8S)-diaminopelargonic acid + S-adenosyl-(4-methylthio-2-oxobutanoic acid)
-
37°C, direct assay with 100 mM N-(2-hydroxyethyl)piperazine-N'-(2-propanesulfonic acid) buffer (pH 8.6), 0.1 mM pyridoxal 5'-phosphate, 1 mM S-adenosyl-L-methionine, 2 microM (S)-8-amino-7-oxononanoic acid
-
-
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
i.e. pimeloyl-CoA
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
reaction in biotin biosynthesis
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
i.e. pimeloyl-CoA
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
reaction in biotin biosynthesis
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
i.e. pimeloyl-CoA
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
reaction in biotin biosynthesis
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
i.e. pimeloyl-CoA
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
reaction in biotin biosynthesis
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
Erwinia aroidea
-
i.e. pimeloyl-CoA
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
Erwinia aroidea
-
reaction in biotin biosynthesis
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
-
-
-
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
i.e. pimeloyl-CoA
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
i.e. pimeloyl-CoA
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
i.e. pimeloyl-CoA
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
i.e. pimeloyl-CoA
i.e. 7-oxo-8-amino-pelargonic acid
ir
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
i.e. pimeloyl-CoA
i.e. 7-oxo-8-amino-pelargonic acid
ir
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
i.e. pimeloyl-CoA
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
reaction in biotin biosynthesis
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
reaction in biotin biosynthesis
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
reaction in biotin biosynthesis
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
reaction in biotin biosynthesis
i.e. 7-oxo-8-amino-pelargonic acid
ir
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
reaction in biotin biosynthesis
i.e. 7-oxo-8-amino-pelargonic acid
ir
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
reaction in biotin biosynthesis
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
the enzyme catalyzes the first committed step in the four step pathway, the decarboxylative condensation of L-alanine and pimeloyl-CoA to give (7S)-8-amino-7-oxononanoate
-
-
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
i.e. pimeloyl-CoA
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
reaction in biotin biosynthesis
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
i.e. pimeloyl-CoA
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
reaction in biotin biosynthesis
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
i.e. pimeloyl-CoA
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
reaction in biotin biosynthesis
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
i.e. pimeloyl-CoA
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
i.e. pimeloyl-CoA
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
i.e. pimeloyl-CoA
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
i.e. pimeloyl-CoA
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
i.e. pimeloyl-CoA
i.e. 7-oxo-8-amino-pelargonic acid
ir
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
i.e. pimeloyl-CoA
i.e. 7-oxo-8-amino-pelargonic acid
ir
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
i.e. pimeloyl-CoA
i.e. 7-oxo-8-amino-pelargonic acid
ir
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
second step in biotin biosynthesis
i.e. 7-oxo-8-amino-pelargonic acid
ir
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
second step in biotin biosynthesis
i.e. 7-oxo-8-amino-pelargonic acid
ir
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
reaction in biotin biosynthesis
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
reaction in biotin biosynthesis
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
reaction in biotin biosynthesis
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
reaction in biotin biosynthesis
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
reaction in biotin biosynthesis
i.e. 7-oxo-8-amino-pelargonic acid
ir
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
reaction in biotin biosynthesis
i.e. 7-oxo-8-amino-pelargonic acid
ir
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
reaction in biotin biosynthesis
i.e. 7-oxo-8-amino-pelargonic acid
ir
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
first committed step in the biotin biosynthesis pathway
-
-
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
i.e. pimeloyl-CoA
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
reaction in biotin biosynthesis
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
i.e. pimeloyl-CoA
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
-
reaction in biotin biosynthesis
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
Sporobolomyces coprophilus
-
i.e. pimeloyl-CoA
i.e. 7-oxo-8-amino-pelargonic acid
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
Sporobolomyces coprophilus
-
reaction in biotin biosynthesis
i.e. 7-oxo-8-amino-pelargonic acid
?
pimeloyl-[acyl-carrier protein] + L-alanine
8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein]
-
-
-
?
pimeloyl-[acyl-carrier protein] + L-alanine
8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein]
-
-
-
?
pimeloyl-[acyl-carrier protein] + L-alanine
8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein]
-
-
-
-
?
pimeloyl-[acyl-carrier protein] + L-alanine
8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein]
-
-
-
?
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(R)-8-amino-7-oxononanoic acid
1,10-phenanthroline
-
to some extent
2,3-dichloronaphthalene-1,4-dione
-
-
2-(furan-2-yl)-1H-benzimidazole
-
-
2-amino-3-hydroxy-2-methylnonadioic acid
-
competitive, transition state analogue
2-[(1,1,2,2-tetrachloroethyl)sulfanyl]-3a,4,7,7a-tetrahydro-1H-isoindole-1,3(2H)-dione
-
-
2-[(trichloromethyl)sulfanyl]-3a,4,7,7a-tetrahydro-1H-isoindole-1,3(2H)-dione
-
-
3-([4-[(2-fluorophenyl)amino]phenyl]disulfanyl)-1H-1,2,4-triazole-1-carboxamide
-
3-([4-[(3,4-difluorophenyl)amino]phenyl]disulfanyl)-1H-1,2,4-triazole-1-carboxamide
-
3-([4-[(3,5-difluorophenyl)amino]phenyl]disulfanyl)-1H-1,2,4-triazole-1-carboxamide
-
3-([4-[(4-fluorophenyl)amino]phenyl]disulfanyl)-1H-1,2,4-triazole-1-carboxamide
-
3-([4-[(4-nitrophenyl)amino]phenyl]disulfanyl)-1H-1,2,4-triazole-1-carboxamide
-
3-[[4-(benzylamino)phenyl]disulfanyl]-1H-1,2,4-triazole-1-carboxamide
-
4-(1H-inden-3-yl)naphthalene-1,2-dione
-
-
4-carboxybutyl(1-amino-1-carboxyethyl)phosphonate
-
competitive, transition state analogue
5,10-dioxo-5,10-dihydronaphtho[2,3-b][1,4]dithiine-2,3-dicarbonitrile
-
-
5,8-dihydronaphthalene-1,4-dione
-
-
5,8-dihydroxynaphthalene-1,4-dione
-
-
5-hydroxy-2-methylnaphthalene-1,4-dione
-
-
5-hydroxynaphthalene-1,4-dione
-
-
8-amino-7-oxo-8-phosphonononanoic acid
-
strong inhibition
8-Amino-7-oxononanoate
-
product inhibition in vivo
8-amino-7-oxooctanoic acid
-
achiral analog of substrate, strong inhibitor of both enzyme forms, binding to active site
D-KAPA
-
product inhibition
D-penicillamine
-
L-enantiomer not as effective
diisopropyl fluorophosphate
-
to some extent
isoniazide
-
to some extent
methyl 3-(5,6-dioxo-5,6-dihydronaphthalen-2-yl)propanoate
-
-
methyl 3-[8-(1H-inden-3-yl)-5,6-dioxo-5,6-dihydronaphthalen-2-yl]propanoate
-
-
methyl 3-[8-[(4-fluorophenyl)amino]-5,6-dioxo-5,6-dihydronaphthalen-2-yl]propanoate
-
-
methyl 3-[8-[(4-methylphenyl)amino]-5,6-dioxo-5,6-dihydronaphthalen-2-yl]propanoate
-
-
N-(2-fluorophenyl)-3-(phenyldisulphanyl)-1H-1,2,4-triazole-1-carboxamide
i.e. KHG23844, induces 2.3fold higher L-alanine accumulation in the treated Arabidopsis thaliana plants. 45-day old Arabidopsis thaliana plants are completely killed by the compound. Foliar supplement of 1 mM biotin at 1 and 2 days before KHG23844 application effectively recovers the growth inhibition of plants treated with the inhibitor
N-[dichloro(fluoro)-l4-sulfanyl]-N',N'-dimethyl-N-phenylsulfuric diamide
-
-
N-[dichloro(fluoromethyl)-lambda4-sulfanyl]-N',N'-dimethyl-N-(4-methylphenyl)sulfuric diamide
-
-
naphthalene-1,2-dione
-
-
pyridoxal 5'-phosphate
-
high concentrations
trifluoroalanine
-
binding structure, irreversible suicide inhibition of the enzyme, involves decarboxylative defluorination of the inhibitor-PLP aldimine followed by attack of the conjugated imine by the amino group of the active site lysine to afford a covalently bound difluorinated intermediate, which can subsequently undergo further HF losses and hydrolysis to afford a 2-(pyridoximine phosphate) acetoyl protein adduct
(R)-8-amino-7-oxononanoic acid
-
no inhibition up to 15 microM with 94.6% ee (S)-enantiomer as substrate, beyond that strong inhibitor of both enzyme forms, binding to active site
(R)-8-amino-7-oxononanoic acid
-
no inhibition up to 15 microM with 94.6% ee (S)-enantiomer as substrate, beyond that strong inhibitor of both enzyme forms, binding to active site
biotin
-
-
D-alanine
-
-
D-alanine
-
competitive; Schiff base formation between D- or L-alanine and pyridoxal-5'-phophate in the active site
D-cycloserine
-
-
D/L-histidine
-
-
D/L-histidine
Erwinia aroidea
-
-
glycine
-
-
L-cysteine
-
-
L-serine
-
-
triphenyltin acetate
-
herbicidal activity (foliar treatment) under greenhouse conditions
additional information
triazolyl phenyl disulfides as 8-amino-7-oxononanoate synthase inhibitors, overview
-
additional information
-
triazolyl phenyl disulfides as 8-amino-7-oxononanoate synthase inhibitors, overview
-
additional information
-
concentration above 10 mM of Ca2+, Mg2+, and Mn2+ cause slow precipitation of the enzyme
-
additional information
-
no inhibition by HgCl2, EDTA, 2,2'-dipyridyl, oxalate, KCN, various amino acids
-
additional information
-
inhibition mechanism; no inhibition by 8-amino-7-oxo-8-phosphonononanoic acid
-
additional information
-
no inhibition by D-alanine
-
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Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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0.0017 - 0.0162
(R)-8-amino-7-oxononanoic acid
0.08
2-amino-3-hydroxy-2-methylnonadioic acid
-
-
0.068
4-carboxybutyl(1-amino-1-carboxyethyl)phosphonate
-
-
0.007
8-amino-7-oxo-8-phosphonononanoic acid
-
-
0.0009 - 0.0152
8-amino-7-oxooctanoic acid
0.115
D-KAPA
-
pH 7.7, 25°C, recombinant enzyme
additional information
additional information
-
dimethyl-8-amino-7-oxooctanoic acid, an achiral substrate analogue, does not inhibit the enzyme
-
0.0017
(R)-8-amino-7-oxononanoic acid
-
competitive constant, pyridoxamine 5'-phosphate form of enzyme, coupled assay at 37°C with 100 mM N-(2-hydroxyethyl)piperazine-N'-(2-propanesulfonic acid) buffer (pH 8.6), 10 mM ATP, 50 mM NaHCO3, 10 mM MgCl2, dithiobiotin syntetase, 0.1 mM pyridoxal 5'-phosphate, 2.5 mM S-adenosyl-L-methionine and various concentrations of (S)-8-amino-7-oxononanoic acid, 0.2-19.2 microM inhibitor
0.0033
(R)-8-amino-7-oxononanoic acid
-
uncompetitive constant, pyridoxal 5'-phosphate form of enzyme, coupled assay at 37°C with 100 mM N-(2-hydroxyethyl)piperazine-N'-(2-propanesulfonic acid) buffer (pH 8.6), 10 mM ATP, 50 mM NaHCO3, 10 mM MgCl2, dithiobiotin syntetase, 0.1 mM pyridoxal 5'-phosphate, varicous concentrations of S-adenosyl-L-methionine and 9 microM (S)-8-amino-7-oxononanoic acid, 0.2-20 microM inhibitor
0.0059
(R)-8-amino-7-oxononanoic acid
-
competitive constant, pyridoxal 5'-phosphate form of enzyme, coupled assay at 37°C with 100 mM N-(2-hydroxyethyl)piperazine-N'-(2-propanesulfonic acid) buffer (pH 8.6), 10 mM ATP, 50 mM NaHCO3, 10 mM MgCl2, dithiobiotin syntetase, 0.1 mM pyridoxal 5'-phosphate, varicous concentrations of S-adenosyl-L-methionine and 9 microM (S)-8-amino-7-oxononanoic acid, 0.2-57.3 microM inhibitor
0.0134
(R)-8-amino-7-oxononanoic acid
-
uncompetitive constant, pyridoxal 5'-phosphate form of enzyme, coupled assay at 37°C with 100 mM N-(2-hydroxyethyl)piperazine-N'-(2-propanesulfonic acid) buffer (pH 8.6), 10 mM ATP, 50 mM NaHCO3, 10 mM MgCl2, dithiobiotin syntetase, 0.1 mM pyridoxal 5'-phosphate, varicous concentrations of S-adenosyl-L-methionine and 9 microM (S)-8-amino-7-oxononanoic acid, 0.2-57.3 microM inhibitor
0.0153
(R)-8-amino-7-oxononanoic acid
-
competitive constant, pyridoxal 5'-phosphate form of enzyme, coupled assay at 37°C with 100 mM N-(2-hydroxyethyl)piperazine-N'-(2-propanesulfonic acid) buffer (pH 8.6), 10 mM ATP, 50 mM NaHCO3, 10 mM MgCl2, dithiobiotin syntetase, 0.1 mM pyridoxal 5'-phosphate, varicous concentrations of S-adenosyl-L-methionine and 9 microM (S)-8-amino-7-oxononanoic acid, 0.2-20 microM inhibitor
0.0162
(R)-8-amino-7-oxononanoic acid
-
uncompetitive constant, pyridoxamine 5'-phosphate form of enzyme, coupled assay at 37°C with 100 mM N-(2-hydroxyethyl)piperazine-N'-(2-propanesulfonic acid) buffer (pH 8.6), 10 mM ATP, 50 mM NaHCO3, 10 mM MgCl2, dithiobiotin syntetase, 0.1 mM pyridoxal 5'-phosphate, 2.5 mM S-adenosyl-L-methionine and various concentrations of (S)-8-amino-7-oxononanoic acid, 0.2-19.2 microM inhibitor
0.0009
8-amino-7-oxooctanoic acid
-
achiral substrate analogue, competitive constant, pyridoxamine 5'-phosphate form of enzyme, coupled assay at 37°C with 100 mM N-(2-hydroxyethyl)piperazine-N'-(2-propanesulfonic acid) buffer (pH 8.6), 10 mM ATP, 50 mM NaHCO3, 10 mM MgCl2, dithiobiotin syntetase, 0.1 mM pyridoxal 5'-phosphate, 2.5 mM S-adenosyl-L-methionine and varicous concentrations of (S)-8-amino-7-oxononanoic acid, 0-100 microM inhibitor
0.0042
8-amino-7-oxooctanoic acid
-
achiral substrate analogue, competitive constant, pyridoxal 5'-phosphate form of enzyme, coupled assay at 37°C with 100 mM N-(2-hydroxyethyl)piperazine-N'-(2-propanesulfonic acid) buffer (pH 8.6), 10 mM ATP, 50 mM NaHCO3, 10 mM MgCl2, dithiobiotin syntetase, 0.1 mM pyridoxal 5'-phosphate, varicous concentrations of S-adenosyl-L-methionine and 9 microM (S)-8-amino-7-oxononanoic acid, 0-60 microM inhibitor
0.0125
8-amino-7-oxooctanoic acid
-
achiral substrate analogue, uncompetitive constant, pyridoxamine 5'-phosphate form of enzyme, coupled assay at 37°C with 100 mM N-(2-hydroxyethyl)piperazine-N'-(2-propanesulfonic acid) buffer (pH 8.6), 10 mM ATP, 50 mM NaHCO3, 10 mM MgCl2, dithiobiotin syntetase, 0.1 mM pyridoxal 5'-phosphate, 2.5 mM S-adenosyl-L-methionine and varicous concentrations of (S)-8-amino-7-oxononanoic acid, 0-100 microM inhibitor
0.0152
8-amino-7-oxooctanoic acid
-
achiral substrate analogue, uncompetitive constant, pyridoxal 5'-phosphate form of enzyme, coupled assay at 37°C with 100 mM N-(2-hydroxyethyl)piperazine-N'-(2-propanesulfonic acid) buffer (pH 8.6), 10 mM ATP, 50 mM NaHCO3, 10 mM MgCl2, dithiobiotin syntetase, 0.1 mM pyridoxal 5'-phosphate, varicous concentrations of S-adenosyl-L-methionine and 9 microM (S)-8-amino-7-oxononanoic acid, 0-60 microM inhibitor
0.59
D-alanine
-
-
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