Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40762 | - |
x * 40763, recombinant enzyme, mass spectrometry, x * 40762, sequence calculation | Bacillus subtilis |
40763 | - |
x * 40763, recombinant enzyme, mass spectrometry, x * 40762, sequence calculation | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycine + O2 + H2O | Bacillus subtilis | - |
glyoxylate + H2O2 + NH3 | - |
? | |
glycine + O2 + H2O | Bacillus subtilis R5 | - |
glyoxylate + H2O2 + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Bacillus subtilis R5 | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
glycine + H2O + O2 = glyoxylate + NH3 + H2O2 | reaction mechanism involving an FADH2-imino acid complex, overview | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycine + O2 + H2O | - |
Bacillus subtilis | glyoxylate + H2O2 + NH3 | - |
? | |
glycine + O2 + H2O | using samples of [2-RS-3H2,2-14C]-, [2-R-3H,2-14C]-, and [2-S-3H,2-14C]glycine, HSi is removed in the overall process. Incubation of the enzyme with [2-RS-3H2,2-14C]glycine under anaerobic conditions, when only the reducing half of the reaction can occur, leads to the recovery of 98.5% of the original glycine, which has the same 3H:14C ratio as the starting substrate. Isotope effects, overview | Bacillus subtilis | glyoxylate + H2O2 + NH3 | - |
? | |
glycine + O2 + H2O | - |
Bacillus subtilis R5 | glyoxylate + H2O2 + NH3 | - |
? | |
glycine + O2 + H2O | using samples of [2-RS-3H2,2-14C]-, [2-R-3H,2-14C]-, and [2-S-3H,2-14C]glycine, HSi is removed in the overall process. Incubation of the enzyme with [2-RS-3H2,2-14C]glycine under anaerobic conditions, when only the reducing half of the reaction can occur, leads to the recovery of 98.5% of the original glycine, which has the same 3H:14C ratio as the starting substrate. Isotope effects, overview | Bacillus subtilis R5 | glyoxylate + H2O2 + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40763, recombinant enzyme, mass spectrometry, x * 40762, sequence calculation | Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | the reaction mechanism involves an FADH2-imino acid complex | Bacillus subtilis |