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2-iminoacetate + H2O
glyoxylate + NH3
cephalosporin C + H2O + O2
?
cyclopropylglycine + H2O + O2
?
D-2-aminobutyrate + H2O + O2
2-oxobutyrate + H2O2
-
as active as sarcosine
-
-
?
D-2-aminobutyrate + H2O + O2
? + NH3 + H2O2
-
2.2% of the activity with sarcosine
-
-
?
D-4-hydroxyphenylglycine + H2O + O2
?
D-Ala + H2O + O2
? + NH3 + H2O2
D-Ala + H2O + O2
pyruvate + NH3 + H2O2
D-alanine + H2O + O2
pyruvate + NH3 + H2O2
D-aminobutanoate + H2O + O2
? + H2O2
30% of the activity with sarcosine
-
-
?
D-Arg + H2O + O2
? + H2O2
-
about 35% of the activity with sarcosine
-
-
?
D-glutamate + H2O + O2
?
-
0.2% activity compared to glycine
-
-
?
D-His + H2O + O2
? + H2O2
-
about 25% of the activity with sarcosine
-
-
?
D-Ile + H2O + O2
3-methyl-2-oxopentanoate + H2O2
-
about 30% of the activity with sarcosine
-
-
?
D-Leu + H2O + O2
4-methyl-2-oxopentanoate + H2O2
-
about 35% of the activity with sarcosine
-
-
?
D-lysine + H2O + O2
?
-
0.2% activity compared to glycine
-
-
?
D-methionine + H2O + O2
4-(methylsulfanyl)-2-oxobutanoic acid + NH3 + H2O2
D-methionine + H2O + O2
?
-
-
-
?
D-norvaline + H2O + O2
2-oxopentanoic acid + NH3 + H2O2
D-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
D-pipecolate + H2O + O2
?
D-pipecolate + H2O + O2
? + H2O2
D-Pro + H2O + O2
? + H2O2
-
about 120% of the activity with sarcosine
-
-
?
D-Pro + H2O + O2
? + NH3 + H2O2
D-proline + H2O + O2
? + NH3 + H2O2
D-Val + H2O + O2
3-methyl-2-oxobutanoate + H2O2
-
about 35% of the activity with sarcosine
-
-
?
D-Val + H2O + O2
? + NH3 + H2O2
-
4.8% of the activity with sarcosine
-
-
?
D-valine + H2O + O2
3-methyl-2-oxobutanoic acid + NH3 + H2O2
glycine + H2O + O2
glyoxylate + NH3 + H2O2
glycine + O2
2-iminoacetate + H2O2
glycine + O2 + H2O
glyoxylate + H2O2 + NH3
glycine ethyl ester + H2O + O2
?
glycine-ethyl-ester + H2O + O2
?
glycine-ethyl-ester + H2O + O2
? + H2O2
glycylglycine + H2O + O2
?
3% activity compared to glycine
-
-
?
glyphosate + H2O + O2
? + H2O2
glyphosate + H2O + O2
glyoxylate + aminomethylphosphonic acid + NH3 + H2O2
N-ethylglycine + H2O + O2
?
N-ethylglycine + H2O + O2
glyoxylate + ethylamine + H2O2
N-methyl-D-Ala + H2O + O2
? + H2O2
75% of the activity with sarcosine
-
-
?
N-methyl-D-Ala + H2O + O2
? + NH3 + H2O2
-
16.9% of the activity with sarcosine
-
-
?
N-methyl-D-Ala + H2O + O2
pyruvate + methylamine + H2O2
-
about 110% of the activity with sarcosine
-
-
?
sarcosine + H2O + O2
glyoxylate + methylamine + H2O2
additional information
?
-
2-iminoacetate + H2O
glyoxylate + NH3
-
-
-
?
2-iminoacetate + H2O
glyoxylate + NH3
-
-
-
-
?
2-iminoacetate + H2O
glyoxylate + NH3
-
-
-
?
2-iminoacetate + H2O
glyoxylate + NH3
-
-
-
?
2-iminoacetate + H2O
glyoxylate + NH3
-
-
-
?
2-iminoacetate + H2O
glyoxylate + NH3
-
-
-
-
?
cephalosporin C + H2O + O2
?
-
-
-
-
?
cephalosporin C + H2O + O2
?
-
-
-
-
?
cyclopropylglycine + H2O + O2
?
-
-
-
?
cyclopropylglycine + H2O + O2
?
enzyme is required for the biosynthesis of the thiazole moiety of thiamine diphosphate
-
-
?
D-4-hydroxyphenylglycine + H2O + O2
?
-
0.6% activity compared to glycine
-
-
?
D-4-hydroxyphenylglycine + H2O + O2
?
-
0.6% activity compared to glycine
-
-
?
D-Ala + H2O + O2
? + NH3 + H2O2
40% of the activity with sarcosine
-
-
?
D-Ala + H2O + O2
? + NH3 + H2O2
-
-
-
-
?
D-Ala + H2O + O2
pyruvate + NH3 + H2O2
-
7.4% of the activity with sarcosine
-
-
?
D-Ala + H2O + O2
pyruvate + NH3 + H2O2
no activity with D-Ala
-
-
?
D-Ala + H2O + O2
pyruvate + NH3 + H2O2
-
about 115% of the activity with sarcosine
-
-
?
D-alanine + H2O + O2
?
-
-
-
?
D-alanine + H2O + O2
?
-
-
-
?
D-alanine + H2O + O2
?
10% activity compared to glycine
-
-
?
D-alanine + H2O + O2
?
10% activity compared to glycine
-
-
?
D-alanine + H2O + O2
pyruvate + NH3 + H2O2
-
-
-
?
D-alanine + H2O + O2
pyruvate + NH3 + H2O2
-
-
-
?
D-alanine + H2O + O2
pyruvate + NH3 + H2O2
-
-
-
-
?
D-alanine + H2O + O2
pyruvate + NH3 + H2O2
-
-
-
?
D-alanine + H2O + O2
pyruvate + NH3 + H2O2
-
-
-
?
D-alanine + H2O + O2
pyruvate + NH3 + H2O2
-
-
-
?
D-alanine + H2O + O2
pyruvate + NH3 + H2O2
-
-
-
-
?
D-alanine + H2O + O2
pyruvate + NH3 + H2O2
-
0.2% activity compared to glycine
-
-
?
D-alanine + H2O + O2
pyruvate + NH3 + H2O2
-
0.2% activity compared to glycine
-
-
?
D-arginine + H2O + O2
?
-
-
-
-
?
D-arginine + H2O + O2
?
-
-
-
-
?
D-aspartate + H2O + O2
?
-
-
-
-
?
D-aspartate + H2O + O2
?
-
-
-
-
?
D-methionine + H2O + O2
4-(methylsulfanyl)-2-oxobutanoic acid + NH3 + H2O2
25% of the activity with sarcosine
-
-
?
D-methionine + H2O + O2
4-(methylsulfanyl)-2-oxobutanoic acid + NH3 + H2O2
-
-
-
-
?
D-methionine + H2O + O2
4-(methylsulfanyl)-2-oxobutanoic acid + NH3 + H2O2
-
-
-
-
?
D-norvaline + H2O + O2
2-oxopentanoic acid + NH3 + H2O2
-
-
-
-
?
D-norvaline + H2O + O2
2-oxopentanoic acid + NH3 + H2O2
-
-
-
-
?
D-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
-
-
-
?
D-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
-
-
-
-
?
D-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
-
-
-
-
?
D-pipecolate + H2O + O2
?
-
-
-
?
D-pipecolate + H2O + O2
?
-
-
-
-
?
D-pipecolate + H2O + O2
?
-
-
-
-
?
D-pipecolate + H2O + O2
? + H2O2
-
about 85% of the activity with sarcosine
-
-
?
D-pipecolate + H2O + O2
? + H2O2
70% of the activity with sarcosine
-
-
?
D-Pro + H2O + O2
? + NH3 + H2O2
-
-
-
-
?
D-Pro + H2O + O2
? + NH3 + H2O2
-
15.1% of the activity with sarcosine
-
-
?
D-Pro + H2O + O2
? + NH3 + H2O2
110% of the activity with sarcosine
-
-
?
D-Pro + H2O + O2
? + NH3 + H2O2
-
-
-
-
?
D-proline + H2O + O2
?
-
-
-
-
?
D-proline + H2O + O2
?
-
-
-
?
D-proline + H2O + O2
?
-
-
-
-
?
D-proline + H2O + O2
?
-
0.3% activity compared to glycine
-
-
?
D-proline + H2O + O2
?
-
0.3% activity compared to glycine
-
-
?
D-proline + H2O + O2
?
25% activity compared to glycine
-
-
?
D-proline + H2O + O2
?
25% activity compared to glycine
-
-
?
D-proline + H2O + O2
? + NH3 + H2O2
-
-
-
?
D-proline + H2O + O2
? + NH3 + H2O2
-
-
-
?
D-valine + H2O + O2
3-methyl-2-oxobutanoic acid + NH3 + H2O2
-
-
-
-
?
D-valine + H2O + O2
3-methyl-2-oxobutanoic acid + NH3 + H2O2
-
-
-
-
?
glycine + H2O + O2
glyoxylate + NH3 + H2O2
-
-
-
-
?
glycine + H2O + O2
glyoxylate + NH3 + H2O2
-
-
-
?
glycine + H2O + O2
glyoxylate + NH3 + H2O2
-
-
-
-
?
glycine + H2O + O2
glyoxylate + NH3 + H2O2
-
-
-
?
glycine + H2O + O2
glyoxylate + NH3 + H2O2
-
-
-
?
glycine + H2O + O2
glyoxylate + NH3 + H2O2
-
-
-
?
glycine + H2O + O2
glyoxylate + NH3 + H2O2
-
-
-
?
glycine + H2O + O2
glyoxylate + NH3 + H2O2
-
-
-
?
glycine + H2O + O2
glyoxylate + NH3 + H2O2
-
-
-
-
?
glycine + H2O + O2
glyoxylate + NH3 + H2O2
-
-
-
?
glycine + H2O + O2
glyoxylate + NH3 + H2O2
-
-
-
-
?
glycine + H2O + O2
glyoxylate + NH3 + H2O2
-
-
-
?
glycine + H2O + O2
glyoxylate + NH3 + H2O2
-
-
-
-
?
glycine + H2O + O2
glyoxylate + NH3 + H2O2
-
-
-
r
glycine + H2O + O2
glyoxylate + NH3 + H2O2
-
free glycine oxidase forms the anionic red semiquinone upon photoreduction.This species is thermodynamically stable, as indicated by the large separation of the two single-electron reduction potentials of DeltaE 290 mV. The first potential is pH-independent, while the second is dependent. The midpoint reduction potential exhibits a 23.4 mV/pH unit slope, which is consistent with an overall two-electrons/one-proton transfer in the reduction to yield anionic reduced flavin. In the presence of glycolate and at pH 7.5 the potential for the semiquinone-reduced enzyme couple is shifted positively by about 160 mV, this favors a two-electron transfer compared to the free enzyme. Binding of glycolate and sulfite is also affected by pH
-
?
glycine + H2O + O2
glyoxylate + NH3 + H2O2
-
77.4% of the activity with sarcosine
-
-
?
glycine + H2O + O2
glyoxylate + NH3 + H2O2
-
as active as sarcosine
-
-
?
glycine + H2O + O2
glyoxylate + NH3 + H2O2
85% of the activity with sarcosine
-
-
?
glycine + H2O + O2
glyoxylate + NH3 + H2O2
-
-
-
?
glycine + H2O + O2
glyoxylate + NH3 + H2O2
-
-
-
r
glycine + H2O + O2
glyoxylate + NH3 + H2O2
-
-
-
-
?
glycine + H2O + O2
glyoxylate + NH3 + H2O2
-
-
-
?
glycine + H2O + O2
glyoxylate + NH3 + H2O2
-
-
-
-
?
glycine + H2O + O2
glyoxylate + NH3 + H2O2
-
-
-
-
?
glycine + H2O + O2
glyoxylate + NH3 + H2O2
-
100% activity
-
-
?
glycine + H2O + O2
glyoxylate + NH3 + H2O2
-
100% activity
-
-
?
glycine + H2O + O2
glyoxylate + NH3 + H2O2
-
-
-
?
glycine + H2O + O2
glyoxylate + NH3 + H2O2
-
-
-
?
glycine + H2O + O2
glyoxylate + NH3 + H2O2
-
-
-
?
glycine + H2O + O2
glyoxylate + NH3 + H2O2
-
-
-
?
glycine + O2
2-iminoacetate + H2O2
-
-
-
?
glycine + O2
2-iminoacetate + H2O2
-
-
-
-
?
glycine + O2
2-iminoacetate + H2O2
-
-
-
?
glycine + O2
2-iminoacetate + H2O2
-
-
-
?
glycine + O2
2-iminoacetate + H2O2
-
-
-
?
glycine + O2
2-iminoacetate + H2O2
-
-
-
?
glycine + O2
2-iminoacetate + H2O2
-
-
-
?
glycine + O2
2-iminoacetate + H2O2
-
-
-
-
?
glycine + O2 + H2O
glyoxylate + H2O2 + NH3
-
-
-
-
?
glycine + O2 + H2O
glyoxylate + H2O2 + NH3
-
using samples of [2-RS-3H2,2-14C]-, [2-R-3H,2-14C]-, and [2-S-3H,2-14C]glycine, HSi is removed in the overall process. Incubation of the enzyme with [2-RS-3H2,2-14C]glycine under anaerobic conditions, when only the reducing half of the reaction can occur, leads to the recovery of 98.5% of the original glycine, which has the same 3H:14C ratio as the starting substrate. Isotope effects, overview
-
-
?
glycine + O2 + H2O
glyoxylate + H2O2 + NH3
-
-
-
-
?
glycine + O2 + H2O
glyoxylate + H2O2 + NH3
-
using samples of [2-RS-3H2,2-14C]-, [2-R-3H,2-14C]-, and [2-S-3H,2-14C]glycine, HSi is removed in the overall process. Incubation of the enzyme with [2-RS-3H2,2-14C]glycine under anaerobic conditions, when only the reducing half of the reaction can occur, leads to the recovery of 98.5% of the original glycine, which has the same 3H:14C ratio as the starting substrate. Isotope effects, overview
-
-
?
glycine ethyl ester + H2O + O2
?
-
30.7% activity compared to glycine
-
-
?
glycine ethyl ester + H2O + O2
?
5% activity compared to glycine
-
-
?
glycine ethyl ester + H2O + O2
?
5% activity compared to glycine
-
-
?
glycine-ethyl-ester + H2O + O2
?
-
-
-
-
?
glycine-ethyl-ester + H2O + O2
?
-
-
-
?
glycine-ethyl-ester + H2O + O2
?
-
-
-
-
?
glycine-ethyl-ester + H2O + O2
? + H2O2
90% of the activity with sarcosine
-
-
?
glycine-ethyl-ester + H2O + O2
? + H2O2
-
-
-
-
?
glyphosate + H2O + O2
?
-
-
-
?
glyphosate + H2O + O2
?
the wild type enzyme shows very low activity with glyphosate
-
-
?
glyphosate + H2O + O2
?
-
-
-
?
glyphosate + H2O + O2
?
-
-
-
?
glyphosate + H2O + O2
?
-
-
-
?
glyphosate + H2O + O2
? + H2O2
-
-
-
-
?
glyphosate + H2O + O2
? + H2O2
110% of the activity with sarcosine
-
-
?
glyphosate + H2O + O2
glyoxylate + aminomethylphosphonic acid + NH3 + H2O2
-
-
-
?
glyphosate + H2O + O2
glyoxylate + aminomethylphosphonic acid + NH3 + H2O2
-
-
-
?
N-ethylglycine + H2O + O2
?
-
0.3% activity compared to glycine
-
-
?
N-ethylglycine + H2O + O2
?
40% activity compared to glycine
-
-
?
N-ethylglycine + H2O + O2
glyoxylate + ethylamine + H2O2
-
-
-
-
?
N-ethylglycine + H2O + O2
glyoxylate + ethylamine + H2O2
-
-
-
?
N-ethylglycine + H2O + O2
glyoxylate + ethylamine + H2O2
-
85.3% of the activity with sarcosine
-
-
?
N-ethylglycine + H2O + O2
glyoxylate + ethylamine + H2O2
-
about 65% of the activity with sarcosine
-
-
?
N-ethylglycine + H2O + O2
glyoxylate + ethylamine + H2O2
120% of the activity with sarcosine
-
-
?
N-ethylglycine + H2O + O2
glyoxylate + ethylamine + H2O2
-
-
-
-
?
N-ethylglycine + H2O + O2
glyoxylate + ethylamine + H2O2
-
-
-
-
?
sarcosine + H2O + O2
?
-
-
-
?
sarcosine + H2O + O2
?
-
-
-
?
sarcosine + H2O + O2
?
-
0.1% activity compared to glycine
-
-
?
sarcosine + H2O + O2
?
-
0.1% activity compared to glycine
-
-
?
sarcosine + H2O + O2
?
-
-
-
?
sarcosine + H2O + O2
?
-
-
-
?
sarcosine + H2O + O2
glyoxylate + methylamine + H2O2
-
-
-
?
sarcosine + H2O + O2
glyoxylate + methylamine + H2O2
-
-
-
-
?
sarcosine + H2O + O2
glyoxylate + methylamine + H2O2
-
-
-
?
sarcosine + H2O + O2
glyoxylate + methylamine + H2O2
-
-
-
-
?
sarcosine + H2O + O2
glyoxylate + methylamine + H2O2
-
-
-
?
sarcosine + H2O + O2
glyoxylate + methylamine + H2O2
-
-
-
-
?
sarcosine + H2O + O2
glyoxylate + methylamine + H2O2
-
-
-
?
sarcosine + H2O + O2
glyoxylate + methylamine + H2O2
-
-
-
?
sarcosine + H2O + O2
glyoxylate + methylamine + H2O2
-
-
-
-
?
sarcosine + H2O + O2
glyoxylate + methylamine + H2O2
-
-
-
-
?
additional information
?
-
-
glycine oxidase is converted to a two-electron reduced form upon anaerobic reduction with the individual substrates and its reductive half-reaction is reversible
-
-
?
additional information
?
-
a general catabolic role of the enzyme on primary or secondary amines is excluded, because the expression of glycine oxidase is not inducible by Gly, sarcosine, or D-Ala as carbon or nitrogen source
-
-
?
additional information
?
-
-
a general catabolic role of the enzyme on primary or secondary amines is excluded, because the expression of glycine oxidase is not inducible by Gly, sarcosine, or D-Ala as carbon or nitrogen source
-
-
?
additional information
?
-
-
the enzyme is strictly stereospecific as it only catalyzes the oxidation of the D-isomer
-
-
?
additional information
?
-
no activity with L-alanine
-
-
?
additional information
?
-
no activity with L-alanine
-
-
?
additional information
?
-
-
no activity with butilamine, benzilamine, D-histidine, D-leucine, L-proline, D-tryptophan, and D-tyrosine
-
-
?
additional information
?
-
-
substrate preference for amines of small size, like glycine, sarcosine, N-ethyl-glycine, and glycine-ethylester
-
-
?
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1.17
cyclopropylglycine
-
221.8 - 2000
D-pipecolate
0.29 - 1.8
glycine-ethyl-ester
0.71 - 2.8
N-ethyl-glycine
0.22 - 7.68
N-ethylglycine
4 - 10
D-alanine
-
apparent value for mutant enzyme M261Y, at 25°C and pH 8.5
20.6
D-alanine
-
pH 8.5, 37°C
20.6
D-alanine
-
in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate, at pH 8.5 and 37°C
34.65
D-alanine
wild type enzyme, at pH 7.5, temperature not specified in the publication
45.54
D-alanine
pH 8.2, 23°C
121.9
D-alanine
-
M261Y mutant
155.6
D-alanine
-
M261H mutant
217
D-alanine
mutant enzyme I15V, in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate (pH 8.5) at 37°C
315
D-alanine
-
M261 wild type
315
D-alanine
wild type enzyme, in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate (pH 8.5) at 37°C
350
D-alanine
-
apparent value for mutant enzyme H244Q, at 25°C and pH 8.5
420
D-alanine
-
apparent value for mutant enzyme H244N, at 25°C and pH 8.5
490
D-alanine
-
apparent value for wild type enzyme, at 25°C and pH 8.5
530
D-alanine
-
apparent value for mutant enzyme H244F, at 25°C and pH 8.5
2000
D-alanine
-
Km above 2000 mM, apparent value for mutant enzyme M261H, at 25°C and pH 8.5
221.8
D-pipecolate
-
pH 8.5, 37°C
221.8
D-pipecolate
-
in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate, at pH 8.5 and 37°C
290
D-pipecolate
mutant enzyme I15V, in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate (pH 8.5) at 37°C
2000
D-pipecolate
wild type enzyme, in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate (pH 8.5) at 37°C
46
D-Pro
-
-
2.06
D-proline
-
M261Y mutant
3.91
D-proline
-
M261H mutant
7.9
D-proline
mutant enzyme I15V, in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate (pH 8.5) at 37°C
8.58
D-proline
pH 8.2, 23°C
8.9
D-proline
-
M261 wild type
8.9
D-proline
wild type enzyme, in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate (pH 8.5) at 37°C
9.3
D-proline
-
pH 8.5, 37°C
9.3
D-proline
-
in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate, at pH 8.5 and 37°C
17
D-proline
-
apparent value for wild type enzyme, at 25°C and pH 8.5
26
D-proline
-
apparent value for mutant enzyme H244F, at 25°C and pH 8.5
26
D-proline
-
apparent value for mutant enzyme H244N, at 25°C and pH 8.5
27
D-proline
-
apparent value for mutant enzyme H244Q, at 25°C and pH 8.5
31.7
D-proline
at pH 8.5 and 40°C
280
D-proline
-
apparent value for mutant enzyme M261H, at 25°C and pH 8.5
285
D-proline
-
apparent value for mutant enzyme M261Y, at 25°C and pH 8.5
0.99
Gly
-
-
0.047
glycine
pH 8.5, 37°C, mutant enzyme T42A/C245S/L301V
0.14
glycine
mutant enzyme H244K, at pH 8.5 and 25°C
0.18
glycine
-
M261Y mutant
0.24
glycine
mutant enzyme H244R, at pH 8.5 and 25°C
0.25
glycine
-
pH 8.5, 37°C
0.25
glycine
-
in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate, at pH 8.5 and 37°C
0.3
glycine
mutant enzyme H244K/M261R, at pH 8.5 and 25°C
0.31
glycine
mutant enzyme M49I, at pH 8.5 and 25°C
0.37
glycine
at pH 8.0 and 50°C
0.4
glycine
mutant enzyme I15V, in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate (pH 8.5) at 37°C
0.53
glycine
mutant enzyme N336R, at pH 7.5, temperature not specified in the publication
0.56
glycine
-
M261 wild type
0.6
glycine
-
apparent value for mutant enzyme H244F, at 25°C and pH 8.5
0.6
glycine
wild type enzyme, in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate (pH 8.5) at 37°C
0.7
glycine
wild-type, pH 8.5, 25°C
0.7
glycine
-
apparent value for wild type enzyme, at 25°C and pH 8.5
0.7
glycine
wild type enzyme, at pH 8.5 and 25°C
0.79
glycine
mutant enzyme N336K, at pH 7.5, temperature not specified in the publication
0.8
glycine
-
apparent value for mutant enzyme H244Q, at 25°C and pH 8.5
0.826
glycine
-
mutant enzyme F237A, at 30°C, pH not specified in the publication
0.9
glycine
mutant enzyme H244Q, at pH 8.5 and 25°C
0.95
glycine
mutant enzyme N336H, at pH 7.5, temperature not specified in the publication
0.97
glycine
-
M261H mutant
0.99
glycine
pH 8.5, 37°C, wild-type enzyme
0.99
glycine
mutant enzyme D60G, at pH 8.5 and 60°C
1.04
glycine
-
wild-type, pH 7, 25°C
1.04
glycine
wild type enzyme, at pH 7.5, temperature not specified in the publication
1.04
glycine
wild type enzyme, at pH 8.5 and 60°C
1.35
glycine
mutant enzyme M49L, at pH 8.5 and 25°C
1.41
glycine
mutant enzyme N336A, at pH 7.5, temperature not specified in the publication
1.47
glycine
mutant enzyme M49T, at pH 8.5 and 25°C
1.5
glycine
mutant H244A, pH 8.5, 25°C
1.5
glycine
mutant enzyme H244A, at pH 8.5 and 25°C
1.5
glycine
mutant enzyme M261I, at pH 8.5 and 25°C
1.9
glycine
-
apparent value for mutant enzyme H244N, at 25°C and pH 8.5
1.9
glycine
mutant enzyme H244N, at pH 8.5 and 25°C
2.25
glycine
mutant enzyme N336G, at pH 7.5, temperature not specified in the publication
2.43
glycine
at pH 8.5 and 40°C
3.03
glycine
-
mutant enzyme F237Y, at 30°C, pH not specified in the publication
3.5
glycine
mutant enzyme M261R, at pH 8.5 and 25°C
8.33
glycine
-
pH and temperature not specified in the publication
9.67
glycine
pH 8.2, 23°C
10
glycine
mutant enzyme A54E, at pH 8.5 and 25°C
14.6
glycine
mutant enzyme G51Q, at pH 8.5 and 25°C
14.7
glycine
pH 9.5, 25°C
17.2
glycine
mutant enzyme Y246W, at pH 8.5 and 25°C
18
glycine
-
apparent value for mutant enzyme M261Y, at 25°C and pH 8.5
18.8
glycine
mutant enzyme M49L/A54R/H244K, at pH 8.5 and 25°C
28
glycine
-
apparent value for mutant enzyme M261H, at 25°C and pH 8.5
28
glycine
mutant A54R, pH 8.5, 25°C
28
glycine
mutant enzyme A54R, at pH 8.5 and 25°C
33.47
glycine
-
mutant S122P/H164R/Q346R/E357G/K367R/T368R, pH 7, 25°C
35
glycine
mutant G51S/A54R, pH 8.5, 25°C
35.7
glycine
mutant enzyme G51H, at pH 8.5 and 25°C
38
glycine
mutant Y241H, pH 8.5, 25°C
39
glycine
-
wild type enzyme, at 30°C, pH not specified in the publication
40.1
glycine
mutant enzyme A54R/H244K, at pH 8.5 and 25°C
41.55
glycine
mutant enzyme G51R/G60S/T118A/K133R/I198V/V262I/I284L/L307S/E357G, at pH 8.5 and 60°C
41.64
glycine
mutant enzyme G51R/D60G/T118A/K133R/I284L, at pH 8.5 and 60°C
45.9
glycine
mutant enzyme A54R/H244K/M261R, at pH 8.5 and 25°C
53
glycine
mutant G51R, pH 8.5, 25°C
54.6
glycine
mutant enzyme G51R, at pH 8.5 and 60°C
58.5
glycine
mutant enzyme G51R/D60G, at pH 8.5 and 60°C
59
glycine
mutant G51R/A54R, pH 8.5, 25°C
59.94
glycine
-
mutant D103Y/D121G/S122P/R238K/K367R/T368R, pH 7, 25°C
80.43
glycine
mutant enzyme G51R/G60S/D121G/S122P/H164Y/M267T, at pH 8.5 and 60°C
92.5
glycine
mutant enzyme G51R/D60G/T118A/K133R, at pH 8.5 and 60°C
101.8
glycine
mutant enzyme BG51R/G60S/I198V/E357G, at pH 8.5 and 60°C
105
glycine
mutant G51S/A54R/H244A, pH 8.5, 25°C
105.6
glycine
mutant enzyme G51R/D60G/K133R/V262I, at pH 8.5 and 60°C
134.4
glycine
mutant enzyme BG51R/G60S/S210P/M267T, at pH 8.5 and 60°C
138.1
glycine
mutant enzyme G51R/G60S/K133R/S210P/R250G/V262I, at pH 8.5 and 60°C
0.29
glycine-ethyl-ester
-
M261Y mutant
0.52
glycine-ethyl-ester
-
pH 8.5, 37°C
0.52
glycine-ethyl-ester
-
in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate, at pH 8.5 and 37°C
0.6
glycine-ethyl-ester
mutant enzyme I15V, in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate (pH 8.5) at 37°C
1.57
glycine-ethyl-ester
-
M261H mutant
1.78
glycine-ethyl-ester
-
M261 wild type
1.8
glycine-ethyl-ester
wild type enzyme, in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate (pH 8.5) at 37°C
0.051
glyphosate
-
mutant D103Y/D121G/S122P/R238K/K367R/T368R, pH 7, 25°C
0.061
glyphosate
-
mutant S122P/H164R/Q346R/E357G/K367R/T368R, pH 7, 25°C
0.5
glyphosate
mutant G51S/A54R/H244A, pH 8.5, 25°C
0.53
glyphosate
mutant enzyme G51R/G60S/T118A/K133R/I198V/V262I/I284L/L307S/E357G, at pH 8.5 and 60°C
0.57
glyphosate
mutant enzyme G51R/D60G/T118A/K133R/I284L, at pH 8.5 and 60°C
1
glyphosate
mutant G51R/A54R, pH 8.5, 25°C
1.3
glyphosate
mutant G51S/A54R, pH 8.5, 25°C
1.37
glyphosate
mutant enzyme G51R/G60S/D121G/S122P/H164Y/M267T, at pH 8.5 and 60°C
1.69
glyphosate
mutant enzyme G51R/G60S/K133R/S210P/R250G/V262I, at pH 8.5 and 60°C
2.17
glyphosate
mutant enzyme G51R/D60G/T118A/K133R, at pH 8.5 and 60°C
2.45
glyphosate
mutant enzyme G51R/D60G, at pH 8.5 and 60°C
2.77
glyphosate
mutant enzyme G51R/D60G/K133R/V262I, at pH 8.5 and 60°C
3.8
glyphosate
mutant enzyme BG51R/G60S/I198V/E357G, at pH 8.5 and 60°C
4.37
glyphosate
mutant enzyme BG51R/G60S/S210P/M267T, at pH 8.5 and 60°C
4.4
glyphosate
mutant A54R, pH 8.5, 25°C
6.5
glyphosate
mutant G51R, pH 8.5, 25°C
8.29
glyphosate
mutant enzyme G51R, at pH 8.5 and 60°C
10.44
glyphosate
mutant enzyme N336R, at pH 7.5, temperature not specified in the publication
18.88
glyphosate
mutant enzyme D60G, at pH 8.5 and 60°C
22.45
glyphosate
mutant enzyme N336K, at pH 7.5, temperature not specified in the publication
41
glyphosate
mutant Y241H, pH 8.5, 25°C
42.31
glyphosate
mutant enzyme N336H, at pH 7.5, temperature not specified in the publication
68.36
glyphosate
mutant enzyme N336A, at pH 7.5, temperature not specified in the publication
78
glyphosate
mutant H244A, pH 8.5, 25°C
84.79
glyphosate
-
wild-type, pH 7, 25°C
84.79
glyphosate
wild type enzyme, at pH 7.5, temperature not specified in the publication
84.79
glyphosate
wild type enzyme, at pH 8.5 and 60°C
87
glyphosate
wild-type, pH 8.5, 25°C
96.73
glyphosate
mutant enzyme N336G, at pH 7.5, temperature not specified in the publication
0.71
N-ethyl-glycine
-
M261Y mutant
2.07
N-ethyl-glycine
-
M261H mutant
2.8
N-ethyl-glycine
-
M261 wild type
0.22
N-ethylglycine
-
pH 8.5, 37°C
0.22
N-ethylglycine
-
in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate, at pH 8.5 and 37°C
0.5
N-ethylglycine
mutant enzyme I15V, in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate (pH 8.5) at 37°C
2.8
N-ethylglycine
wild type enzyme, in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate (pH 8.5) at 37°C
7.68
N-ethylglycine
at pH 8.5 and 40°C
0.15
O2
pH 9.0, 25°C
0.38
O2
-
reaction with Gly
0.416
O2
-
mutant enzyme F237A, at 30°C, pH not specified in the publication
0.42
O2
-
reaction with sarcosine
0.44
O2
-
reaction with D-Pro
2.27
O2
-
mutant enzyme F237Y, at 30°C, pH not specified in the publication
2.6
O2
-
wild type enzyme, at 30°C, pH not specified in the publication
0.22
sarcosine
-
-
0.23
sarcosine
-
M261Y mutant
0.45
sarcosine
-
pH 8.5, 37°C
0.45
sarcosine
-
in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate, at pH 8.5 and 37°C
0.51
sarcosine
mutant enzyme I15V, in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate (pH 8.5) at 37°C
0.57
sarcosine
-
M261 wild type
0.58
sarcosine
-
M261H mutant
0.6
sarcosine
wild type enzyme, in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate (pH 8.5) at 37°C
0.7
sarcosine
-
apparent value for wild type enzyme, at 25°C and pH 8.5
0.7
sarcosine
wild type enzyme, at pH 8.5 and 25°C
1.4
sarcosine
-
apparent value for mutant enzyme H244F, at 25°C and pH 8.5
1.51
sarcosine
wild type enzyme, at pH 7.5, temperature not specified in the publication
2.1
sarcosine
-
apparent value for mutant enzyme H244N, at 25°C and pH 8.5
2.8
sarcosine
-
apparent value for mutant enzyme H244Q, at 25°C and pH 8.5
4.86
sarcosine
at pH 8.5 and 40°C
11
sarcosine
-
apparent value for mutant enzyme M261H, at 25°C and pH 8.5
11
sarcosine
-
apparent value for mutant enzyme M261Y, at 25°C and pH 8.5
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
1.14
cyclopropylglycine
-
0.4
D-Ala
-
0.33
D-alanine
-
kcat above 0.33 1/s, apparent value for mutant enzyme M261H, at 25°C and pH 8.5
0.47
D-alanine
-
apparent value for mutant enzyme M261Y, at 25°C and pH 8.5
0.75
D-alanine
-
apparent value for mutant enzyme H244N, at 25°C and pH 8.5
0.81
D-alanine
wild type enzyme, at pH 7.5, temperature not specified in the publication
0.92
D-alanine
-
apparent value for wild type enzyme, at 25°C and pH 8.5
1.08
D-alanine
-
apparent value for mutant enzyme H244Q, at 25°C and pH 8.5
1.4
D-alanine
pH 8.2, 23°C
1.5
D-alanine
-
apparent value for mutant enzyme H244F, at 25°C and pH 8.5
1.3
D-Pro
-
-
0.3
D-proline
-
apparent value for mutant enzyme H244N, at 25°C and pH 8.5
0.38
D-proline
-
apparent value for mutant enzyme M261Y, at 25°C and pH 8.5
0.5
D-proline
-
apparent value for wild type enzyme, at 25°C and pH 8.5
0.62
D-proline
-
apparent value for mutant enzyme M261H, at 25°C and pH 8.5
1.23
D-proline
-
apparent value for mutant enzyme H244Q, at 25°C and pH 8.5
1.35
D-proline
-
apparent value for mutant enzyme H244F, at 25°C and pH 8.5
1.69
D-proline
pH 8.2, 23°C
1.2
Gly
-
0.007
glycine
mutant enzyme G51R/D60G, at pH 8.5 and 60°C
0.019
glycine
mutant enzyme G51R, at pH 8.5 and 60°C
0.0225
glycine
mutant enzyme G51R/D60G/T118A/K133R, at pH 8.5 and 60°C
0.024
glycine
mutant enzyme N336R, at pH 7.5, temperature not specified in the publication
0.031
glycine
mutant enzyme G51R/D60G/K133R/V262I, at pH 8.5 and 60°C
0.038
glycine
mutant enzyme G51R/D60G/T118A/K133R/I284L, at pH 8.5 and 60°C
0.04
glycine
mutant enzyme M49T, at pH 8.5 and 25°C
0.076
glycine
mutant enzyme D60G, at pH 8.5 and 60°C
0.09
glycine
mutant enzyme BG51R/G60S/S210P/M267T, at pH 8.5 and 60°C
0.09
glycine
mutant enzyme G51R/G60S/T118A/K133R/I198V/V262I/I284L/L307S/E357G, at pH 8.5 and 60°C
0.095
glycine
mutant enzyme G51R/G60S/D121G/S122P/H164Y/M267T, at pH 8.5 and 60°C
0.103
glycine
-
mutant D103Y/D121G/S122P/R238K/K367R/T368R, pH 7, 25°C
0.136
glycine
-
wild-type, pH 7, 25°C
0.14
glycine
wild type enzyme, at pH 8.5 and 60°C
0.158
glycine
-
mutant S122P/H164R/Q346R/E357G/K367R/T368R, pH 7, 25°C
0.169
glycine
mutant enzyme G51R/G60S/K133R/S210P/R250G/V262I, at pH 8.5 and 60°C
0.217
glycine
mutant enzyme BG51R/G60S/I198V/E357G, at pH 8.5 and 60°C
0.25
glycine
mutant enzyme N336K, at pH 7.5, temperature not specified in the publication
0.3
glycine
-
apparent value for mutant enzyme M261Y, at 25°C and pH 8.5
0.3
glycine
pH 8.5, 37°C, mutant enzyme T42A/C245S/L301V
0.35
glycine
mutant G51R, pH 8.5, 25°C
0.35
glycine
mutant enzyme A54E, at pH 8.5 and 25°C
0.36
glycine
mutant enzyme M49I, at pH 8.5 and 25°C
0.37
glycine
mutant enzyme N336H, at pH 7.5, temperature not specified in the publication
0.43
glycine
mutant enzyme M49L, at pH 8.5 and 25°C
0.52
glycine
mutant enzyme N336A, at pH 7.5, temperature not specified in the publication
0.55
glycine
-
apparent value for mutant enzyme M261H, at 25°C and pH 8.5
0.6
glycine
wild-type, pH 8.5, 25°C
0.6
glycine
-
apparent value for wild type enzyme, at 25°C and pH 8.5
0.6
glycine
mutant enzyme M49L/A54R/H244K, at pH 8.5 and 25°C
0.6
glycine
wild type enzyme, at pH 8.5 and 25°C
0.63
glycine
mutant H244A, pH 8.5, 25°C
0.63
glycine
mutant enzyme H244A, at pH 8.5 and 25°C
0.64
glycine
mutant enzyme M261I, at pH 8.5 and 25°C
0.67
glycine
mutant enzyme N336G, at pH 7.5, temperature not specified in the publication
0.7
glycine
mutant G51R/A54R, pH 8.5, 25°C
0.71
glycine
wild type enzyme, at pH 7.5, temperature not specified in the publication
0.74
glycine
mutant enzyme G51Q, at pH 8.5 and 25°C
0.77
glycine
mutant enzyme G51H, at pH 8.5 and 25°C
0.78
glycine
-
apparent value for mutant enzyme H244F, at 25°C and pH 8.5
0.91
glycine
mutant G51S/A54R, pH 8.5, 25°C
0.91
glycine
mutant Y241H, pH 8.5, 25°C
1
glycine
mutant enzyme M261R, at pH 8.5 and 25°C
1.2
glycine
-
apparent value for mutant enzyme H244Q, at 25°C and pH 8.5
1.2
glycine
mutant A54R, pH 8.5, 25°C
1.2
glycine
mutant enzyme A54R, at pH 8.5 and 25°C
1.2
glycine
mutant enzyme H244K/M261R, at pH 8.5 and 25°C
1.21
glycine
mutant enzyme H244Q, at pH 8.5 and 25°C
1.21
glycine
mutant enzyme Y246W, at pH 8.5 and 25°C
1.3
glycine
pH 8.5, 37°C, wild-type enzyme
1.3
glycine
-
apparent value for mutant enzyme H244N, at 25°C and pH 8.5
1.3
glycine
mutant enzyme H244N, at pH 8.5 and 25°C
1.35
glycine
mutant enzyme H244K, at pH 8.5 and 25°C
1.49
glycine
mutant enzyme H244R, at pH 8.5 and 25°C
1.5
glycine
mutant G51S/A54R/H244A, pH 8.5, 25°C
1.61
glycine
mutant enzyme A54R/H244K/M261R, at pH 8.5 and 25°C
2.16
glycine
pH 8.2, 23°C
3.2
glycine
mutant enzyme A54R/H244K, at pH 8.5 and 25°C
39
glycine
-
wild type enzyme, at 30°C, pH not specified in the publication
0.021
glyphosate
mutant enzyme N336R, at pH 7.5, temperature not specified in the publication
0.046
glyphosate
mutant enzyme G51R/D60G, at pH 8.5 and 60°C
0.049
glyphosate
mutant enzyme G51R, at pH 8.5 and 60°C
0.069
glyphosate
mutant enzyme G51R/D60G/T118A/K133R, at pH 8.5 and 60°C
0.077
glyphosate
mutant enzyme G51R/D60G/T118A/K133R/I284L, at pH 8.5 and 60°C
0.095
glyphosate
-
wild-type, pH 7, 25°C
0.095
glyphosate
wild type enzyme, at pH 8.5 and 60°C
0.12
glyphosate
mutant enzyme D60G, at pH 8.5 and 60°C
0.121
glyphosate
mutant enzyme BG51R/G60S/S210P/M267T, at pH 8.5 and 60°C
0.162
glyphosate
-
mutant D103Y/D121G/S122P/R238K/K367R/T368R, pH 7, 25°C
0.19
glyphosate
mutant enzyme N336K, at pH 7.5, temperature not specified in the publication
0.19
glyphosate
-
mutant S122P/H164R/Q346R/E357G/K367R/T368R, pH 7, 25°C
0.195
glyphosate
mutant enzyme G51R/G60S/T118A/K133R/I198V/V262I/I284L/L307S/E357G, at pH 8.5 and 60°C
0.2165
glyphosate
mutant enzyme G51R/G60S/D121G/S122P/H164Y/M267T, at pH 8.5 and 60°C
0.22
glyphosate
mutant enzyme G51R/G60S/K133R/S210P/R250G/V262I, at pH 8.5 and 60°C
0.23
glyphosate
mutant enzyme N336H, at pH 7.5, temperature not specified in the publication
0.513
glyphosate
mutant enzyme BG51R/G60S/I198V/E357G, at pH 8.5 and 60°C
0.62
glyphosate
mutant enzyme N336G, at pH 7.5, temperature not specified in the publication
0.67
glyphosate
mutant enzyme N336A, at pH 7.5, temperature not specified in the publication
0.7
glyphosate
mutant G51R/A54R, pH 8.5, 25°C
0.77
glyphosate
mutant H244A, pH 8.5, 25°C
0.87
glyphosate
wild type enzyme, at pH 7.5, temperature not specified in the publication
0.91
glyphosate
wild-type, pH 8.5, 25°C
1.05
glyphosate
mutant G51S/A54R, pH 8.5, 25°C
1.05
glyphosate
mutant G51S/A54R/H244A, pH 8.5, 25°C
1.3
glyphosate
mutant Y241H, pH 8.5, 25°C
1.5
glyphosate
mutant A54R, pH 8.5, 25°C
1.8
glyphosate
mutant G51R, pH 8.5, 25°C
64
glyphosate
mutant enzyme G51R/D60G/K133R/V262I, at pH 8.5 and 60°C
0.6
O2
-
mutant enzyme F237A, at 30°C, pH not specified in the publication
15
O2
-
mutant enzyme F237Y, at 30°C, pH not specified in the publication
93
O2
-
wild type enzyme, at 30°C, pH not specified in the publication
0.47
sarcosine
-
apparent value for mutant enzyme M261Y, at 25°C and pH 8.5
0.6
sarcosine
-
apparent value for wild type enzyme, at 25°C and pH 8.5
0.6
sarcosine
wild type enzyme, at pH 8.5 and 25°C
0.73
sarcosine
-
apparent value for mutant enzyme M261H, at 25°C and pH 8.5
0.98
sarcosine
wild type enzyme, at pH 7.5, temperature not specified in the publication
1.08
sarcosine
-
apparent value for mutant enzyme H244F, at 25°C and pH 8.5
1.12
sarcosine
-
apparent value for mutant enzyme H244Q, at 25°C and pH 8.5
1.33
sarcosine
-
apparent value for mutant enzyme H244N, at 25°C and pH 8.5
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0.023
D-alanine
wild type enzyme, at pH 7.5, temperature not specified in the publication
0.02
glycine
mutant enzyme G51H, at pH 8.5 and 25°C
0.03
glycine
mutant enzyme M49L/A54R/H244K, at pH 8.5 and 25°C
0.03
glycine
mutant enzyme M49T, at pH 8.5 and 25°C
0.04
glycine
mutant enzyme A54E, at pH 8.5 and 25°C
0.04
glycine
mutant enzyme A54R, at pH 8.5 and 25°C
0.04
glycine
mutant enzyme A54R/H244K/M261R, at pH 8.5 and 25°C
0.045
glycine
mutant enzyme N336R, at pH 7.5, temperature not specified in the publication
0.05
glycine
mutant enzyme G51Q, at pH 8.5 and 25°C
0.07
glycine
mutant enzyme Y246W, at pH 8.5 and 25°C
0.11
glycine
mutant enzyme A54R/H244K, at pH 8.5 and 25°C
0.3
glycine
mutant enzyme M261R, at pH 8.5 and 25°C
0.3
glycine
mutant enzyme N336G, at pH 7.5, temperature not specified in the publication
0.32
glycine
mutant enzyme M49L, at pH 8.5 and 25°C
0.32
glycine
mutant enzyme N336K, at pH 7.5, temperature not specified in the publication
0.37
glycine
mutant enzyme N336A, at pH 7.5, temperature not specified in the publication
0.39
glycine
mutant enzyme N336H, at pH 7.5, temperature not specified in the publication
0.42
glycine
mutant enzyme H244A, at pH 8.5 and 25°C
0.43
glycine
mutant enzyme M261I, at pH 8.5 and 25°C
0.68
glycine
wild type enzyme, at pH 7.5, temperature not specified in the publication
0.68
glycine
mutant enzyme H244N, at pH 8.5 and 25°C
0.86
glycine
wild type enzyme, at pH 8.5 and 25°C
1.17
glycine
mutant enzyme M49I, at pH 8.5 and 25°C
1.3
glycine
pH 8.5, 37°C, wild-type enzyme
1.34
glycine
mutant enzyme H244Q, at pH 8.5 and 25°C
4
glycine
mutant enzyme H244K/M261R, at pH 8.5 and 25°C
6.22
glycine
mutant enzyme H244R, at pH 8.5 and 25°C
6.4
glycine
pH 8.5, 37°C, mutant enzyme T42A/C245S/L301V
9.65
glycine
mutant enzyme H244K, at pH 8.5 and 25°C
0.002
glyphosate
mutant enzyme N336R, at pH 7.5, temperature not specified in the publication
0.0054
glyphosate
mutant enzyme N336H, at pH 7.5, temperature not specified in the publication
0.0064
glyphosate
mutant enzyme N336G, at pH 7.5, temperature not specified in the publication
0.0084
glyphosate
mutant enzyme N336K, at pH 7.5, temperature not specified in the publication
0.0098
glyphosate
mutant enzyme N336A, at pH 7.5, temperature not specified in the publication
0.01
glyphosate
wild type enzyme, at pH 7.5, temperature not specified in the publication
1
O2
-
mutant enzyme F237A, at 30°C, pH not specified in the publication
6.6
O2
-
mutant enzyme F237Y, at 30°C, pH not specified in the publication
36
O2
-
wild type enzyme, at 30°C, pH not specified in the publication
0.65
sarcosine
wild type enzyme, at pH 7.5, temperature not specified in the publication
0.86
sarcosine
wild type enzyme, at pH 8.5 and 25°C
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BG51R/G60S/I198V/E357G
the mutant shows no activity with glycine and high activity with glyphosate
BG51R/G60S/S210P/M267T
the mutant shows no activity with glycine and activity with glyphosate
D103Y/D121G/S122P/R238K/K367R/T368R
-
mutant displays increased activity towards glyphosate with dramatic loss of activity with glycine
D60G
the mutant shows activity with glycine and very low activity with glyphosate
G51R
the mutant shows no activity with glycine and very low activity with glyphosate
G51R/D60G
the mutant shows no activity with glycine and activity with glyphosate
G51R/D60G/K133R/V262I
the mutant shows no activity with glycine and activity with glyphosate
G51R/D60G/T118A/K133R
the mutant shows no activity with glycine and activity with glyphosate
G51R/D60G/T118A/K133R/I284L
the mutant shows no activity with glycine and very high activity with glyphosate
G51R/G60S/D121G/S122P/H164Y/M267T
the mutant shows no activity with glycine and high activity with glyphosate
G51R/G60S/K133R/S210P/R250G/V262I
the mutant shows no activity with glycine and high activity with glyphosate
G51R/G60S/T118A/K133R/I198V/V262I/I284L/L307S/E357G
the mutant shows no activity with glycine and high activity with glyphosate
N336A
the mutant shows increased substrate affinity compared to the wild type enzyme
N336G
the mutant shows increased substrate affinity compared to the wild type enzyme
N336H
the mutant shows increased substrate affinity compared to the wild type enzyme
N336K
the mutant shows increased substrate affinity compared to the wild type enzyme
N336R
the mutant shows increased substrate affinity compared to the wild type enzyme
S122P/H164R/Q346R/E357G/K367R/T368R
-
mutant displays increased activity towards glyphosate with dramatic loss of activity with glycine
N336A
-
the mutant shows increased substrate affinity compared to the wild type enzyme
-
N336G
-
the mutant shows increased substrate affinity compared to the wild type enzyme
-
N336H
-
the mutant shows increased substrate affinity compared to the wild type enzyme
-
N336K
-
the mutant shows increased substrate affinity compared to the wild type enzyme
-
N336R
-
the mutant shows increased substrate affinity compared to the wild type enzyme
-
A45H/G300C
monomeric in solution. Upon incubation of apoprotein with FAD, neither FAD binding nor enzymatic activity is observed. Slow elimination of urea from partially unfolded mutant in presence of a large excess of FAD and 30% glyccerol does not produce the holoenzyme
A54E
the mutant shows reduced catalytic efficiency with glycine compared to the wild type enzyme
A54R/H244K
the mutant shows reduced catalytic efficiency with glycine compared to the wild type enzyme
A54R/H244K/M261R
the mutant shows reduced catalytic efficiency with glycine compared to the wild type enzyme
agriculture
expression of an evolved engineered variant of glycine oxidase leads to glyphosate resistance in alfalfa
G300C
upon incubation of apoprotein with FAD, neither FAD binding nor enzymatic activity is observed. Slow elimination of urea from partially unfolded mutant in presence of a large excess of FAD and 30% glycerol does not produce the holoenzyme
G51H
the mutant shows reduced catalytic efficiency with glycine compared to the wild type enzyme
G51Q
the mutant shows reduced catalytic efficiency with glycine compared to the wild type enzyme
G51R
4000fold increase in the specificity constant for substrate glyphosate
G51R/A54R
5800fold increase in the specificity constant for substrate glyphosate
G51S/A54R
3100fold increase in the specificity constant for substrate glyphosate
G51S/A54R/H244A
210fold increase in catalytic activity and 15000fold increase in the specificity constant for substrate glyphosate. The alpha2-alpha3-loop assumes a different conformation, residue R54 may be the key residue in stabilizing glyphosate binding
H244F
-
shows increased kcat value for glycine compared to the wild type enzyme, the mutation does not affect the expression of glycine oxidase and the physicochemical properties of bound FAD
H244K
the mutant shows increased catalytic efficiency with glycine compared to the wild type enzyme
H244K/M261R
the variant shows a 5.4fold increase in maximal activity on glycine compared to the wild type enzyme
H244R
the mutant shows increased catalytic efficiency with glycine compared to the wild type enzyme
I15V
the mutant exhibits a 7fold higher specific activity compared to the wild type enzyme
L301V
improvement in residual activity after incubation of 60 min at 60°C to 12.9%
M261I
the mutant shows reduced catalytic efficiency with glycine compared to the wild type enzyme
M261R
the mutant shows reduced catalytic efficiency with glycine compared to the wild type enzyme
M49I
the mutant shows increased catalytic efficiency with glycine compared to the wild type enzyme
M49L
the mutant shows reduced catalytic efficiency with glycine compared to the wild type enzyme
M49L/A54R/H244K
the mutant shows reduced catalytic efficiency with glycine compared to the wild type enzyme
M49T
the mutant shows reduced catalytic efficiency with glycine compared to the wild type enzyme
T42A
improvement in residual activity after incubation of 60 min at 60°C to 18.7%
T42A/C245S/L301V
improvement in residual activity after incubation of 60 min at 60°C to 74.0%. The mutant enzyme retains most of its enzymatic activity during storage for over a year at 4°C
T42S
improvement in residual activity after incubation of 60 min at 60°C to 31.5%
T42S/C245S/L301V
improvement in residual activity after incubation of 60 min at 60°C to 55.7%
Y241H
100fold increase in the specificity constant for substrate glyphosate
Y246W
the mutant shows reduced catalytic efficiency with glycine compared to the wild type enzyme
A54R
-
the mutant shows reduced catalytic efficiency with glycine compared to the wild type enzyme
-
agriculture
-
expression of an evolved engineered variant of glycine oxidase leads to glyphosate resistance in alfalfa
-
H244A
-
the mutant shows reduced catalytic efficiency with glycine compared to the wild type enzyme
-
H244N
-
the mutant shows reduced catalytic efficiency with glycine compared to the wild type enzyme
-
H244Q
-
the mutant shows increased catalytic efficiency with glycine compared to the wild type enzyme
-
L301V
-
improvement in residual activity after incubation of 60 min at 60°C to 12.9%
-
M49L
-
the mutant shows reduced catalytic efficiency with glycine compared to the wild type enzyme
-
T42A
-
improvement in residual activity after incubation of 60 min at 60°C to 18.7%
-
T42A/C245S/L301V
-
improvement in residual activity after incubation of 60 min at 60°C to 74.0%. The mutant enzyme retains most of its enzymatic activity during storage for over a year at 4°C
-
T42S
-
improvement in residual activity after incubation of 60 min at 60°C to 31.5%
-
T42S/C245S/L301V
-
improvement in residual activity after incubation of 60 min at 60°C to 55.7%
-
F237A
-
the mutant shows stronlgy reduced catalytic efficiency compared to the wild type enzyme
F237Y
-
the mutant shows reduced catalytic efficiency compared to the wild type enzyme
A54R
20fold increase in Km for glyphosate
A54R
the mutant shows reduced catalytic efficiency with glycine compared to the wild type enzyme
H244A
2fold increase in expression yield
H244A
the mutant shows reduced catalytic efficiency with glycine compared to the wild type enzyme
H244N
-
shows increased kcat value for glycine compared to the wild type enzyme, the mutation does not affect the expression of glycine oxidase and the physicochemical properties of bound FAD
H244N
the mutant shows reduced catalytic efficiency with glycine compared to the wild type enzyme
H244Q
-
shows increased kcat value compared to the wild type enzyme, the mutation does not affect the expression of glycine oxidase and the physicochemical properties of bound FAD
H244Q
the mutant shows increased catalytic efficiency with glycine compared to the wild type enzyme
M261H
-
decreased in Km compared with the wild type
M261H
-
shows decreased kcat value for glycine compared to the wild type enzyme, the mutation does not affect the expression of glycine oxidase and the physicochemical properties of bound FAD
M261Y
-
decreased in Km compared with the wild type
M261Y
-
shows decreased kcat value for glycine compared to the wild type enzyme, the mutation does not affect the expression of glycine oxidase and the physicochemical properties of bound FAD
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Glyphosate resistance by engineering the flavoenzyme glycine oxidase
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Characterization and structural modeling of a novel thermostable glycine oxidase from Geobacillus kaustophilus HTA426
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Gene cloning and characterization of glycine oxidase from newly isolated Bacillus subtilis strain R5
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-
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Expression of an evolved engineered variant of a bacterial glycine oxidase leads to glyphosate resistance in alfalfa
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674
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Pseudoalteromonas luteoviolacea (A0A161XU12), Pseudoalteromonas luteoviolacea, Pseudoalteromonas luteoviolacea DSM 6061 (A0A161XU12)
brenda
Seok, J.; Kim, Y.J.; Kim, I.K.; Kim, K.J.
Structural basis for stereospecificity to D-amino acid of glycine oxidase from Bacillus cereus ATCC 14579
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Structure and enzymatic properties of an unusual cysteine tryptophylquinone-dependent glycine oxidase from Pseudoalteromonas luteoviolacea
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The Redox properties of a cysteine tryptophylquinone-dependent glycine oxidase are distinct from those of tryptophylquinone-dependent dehydrogenases
Biochemistry
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