Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Geobacillus kaustophilus HTA426 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
N-ethylglycine | pH 8.5, 37°C | Geobacillus kaustophilus HTA426 | |
0.25 | - |
glycine | pH 8.5, 37°C | Geobacillus kaustophilus HTA426 | |
0.45 | - |
sarcosine | pH 8.5, 37°C | Geobacillus kaustophilus HTA426 | |
0.52 | - |
glycine-ethyl-ester | pH 8.5, 37°C | Geobacillus kaustophilus HTA426 | |
9.3 | - |
D-proline | pH 8.5, 37°C | Geobacillus kaustophilus HTA426 | |
20.6 | - |
D-alanine | pH 8.5, 37°C | Geobacillus kaustophilus HTA426 | |
221.8 | - |
D-pipecolate | pH 8.5, 37°C | Geobacillus kaustophilus HTA426 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
4 * 43000, SDS-PAGE | Geobacillus kaustophilus HTA426 |
160000 | - |
gel filtration | Geobacillus kaustophilus HTA426 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus kaustophilus HTA426 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
11.85 | - |
pH 8.5, 37°C | Geobacillus kaustophilus HTA426 |
Storage Stability | Organism |
---|---|
-80°C, 75 mM sodium diphosphate, pH 8.5, 10% glycerol, stable for months | Geobacillus kaustophilus HTA426 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cephalosporin C + H2O + O2 | - |
Geobacillus kaustophilus HTA426 | ? | - |
? | |
D-Ala + H2O + O2 | - |
Geobacillus kaustophilus HTA426 | ? + NH3 + H2O2 | - |
? | |
D-arginine + H2O + O2 | - |
Geobacillus kaustophilus HTA426 | ? | - |
? | |
D-aspartate + H2O + O2 | - |
Geobacillus kaustophilus HTA426 | ? | - |
? | |
D-methionine + H2O + O2 | - |
Geobacillus kaustophilus HTA426 | 4-(methylsulfanyl)-2-oxobutanoic acid + NH3 + H2O2 | - |
? | |
D-norvaline + H2O + O2 | - |
Geobacillus kaustophilus HTA426 | 2-oxopentanoic acid + NH3 + H2O2 | - |
? | |
D-phenylalanine + H2O + O2 | - |
Geobacillus kaustophilus HTA426 | phenylpyruvate + NH3 + H2O2 | - |
? | |
D-pipecolate + H2O + O2 | - |
Geobacillus kaustophilus HTA426 | ? | - |
? | |
D-Pro + H2O + O2 | - |
Geobacillus kaustophilus HTA426 | ? + NH3 + H2O2 | - |
? | |
D-valine + H2O + O2 | - |
Geobacillus kaustophilus HTA426 | 3-methyl-2-oxobutanoic acid + NH3 + H2O2 | - |
? | |
glycine + H2O + O2 | - |
Geobacillus kaustophilus HTA426 | glyoxylate + NH3 + H2O2 | - |
? | |
glycine-ethyl-ester + H2O + O2 | - |
Geobacillus kaustophilus HTA426 | ? + H2O2 | - |
? | |
additional information | substrate preference for amines of small size, like glycine, sarcosine, N-ethyl-glycine, and glycine-ethylester | Geobacillus kaustophilus HTA426 | ? | - |
? | |
N-ethylglycine + H2O + O2 | - |
Geobacillus kaustophilus HTA426 | glyoxylate + ethylamine + H2O2 | - |
? | |
sarcosine + H2O + O2 | - |
Geobacillus kaustophilus HTA426 | glyoxylate + methylamine + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 43000, SDS-PAGE | Geobacillus kaustophilus HTA426 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
74 | - |
- |
Geobacillus kaustophilus HTA426 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
activation during the first 15 min, with slow decrease thereafter | Geobacillus kaustophilus HTA426 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
half-life 0.6 h | Geobacillus kaustophilus HTA426 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Geobacillus kaustophilus HTA426 |