Application | Comment | Organism |
---|---|---|
analysis | the substrate preference of AtcABC enables the application of atcABC genes as molecular biomarkers to assess anaerobic androgen biodegradation in the environment | Steroidobacter denitrificans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.052 | - |
1-testosterone | pH 7.5, 30°C | Steroidobacter denitrificans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mo2+ | a molybdenum enzyme | Steroidobacter denitrificans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-testosterone + H2O + acceptor | Steroidobacter denitrificans | the enzyme is involved in the anaerobic degradation of steroid A-ring | 1,17-dihydroxy-androstan-3-one + reduced acceptor | - |
? | |
1-testosterone + H2O + acceptor | Steroidobacter denitrificans DSM 18526 | the enzyme is involved in the anaerobic degradation of steroid A-ring | 1,17-dihydroxy-androstan-3-one + reduced acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Steroidobacter denitrificans | A0A127F6U7 AND A0A127F985 AND A0A127F9C1 | A0A127F6U7: AtcA, A0A127F985: AtcB,A0A127F9C1: AtcC | - |
Steroidobacter denitrificans DSM 18526 | A0A127F6U7 AND A0A127F985 AND A0A127F9C1 | A0A127F6U7: AtcA, A0A127F985: AtcB,A0A127F9C1: AtcC | - |
Purification (Comment) | Organism |
---|---|
- |
Steroidobacter denitrificans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-dehydrotestosterone + H2O + 2,6-dichlorophenolindophenol | at about 10% of the activity as compared to 1-testosterone | Steroidobacter denitrificans | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
1-dehydrotestosterone + H2O + 2,6-dichlorophenolindophenol | at about 10% of the activity as compared to 1-testosterone | Steroidobacter denitrificans DSM 18526 | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
1-testosterone + H2O + 2,6-dichlorophenolindophenol | - |
Steroidobacter denitrificans | 1,17-dihydroxy-androstan-3-one + reduced 2,6-dichlorophenolindophenol | - |
? | |
1-testosterone + H2O + 2,6-dichlorophenolindophenol | - |
Steroidobacter denitrificans DSM 18526 | 1,17-dihydroxy-androstan-3-one + reduced 2,6-dichlorophenolindophenol | - |
? | |
1-testosterone + H2O + acceptor | the enzyme is involved in the anaerobic degradation of steroid A-ring | Steroidobacter denitrificans | 1,17-dihydroxy-androstan-3-one + reduced acceptor | - |
? | |
1-testosterone + H2O + acceptor | the enzyme is involved in the anaerobic degradation of steroid A-ring | Steroidobacter denitrificans DSM 18526 | 1,17-dihydroxy-androstan-3-one + reduced acceptor | - |
? | |
2-octenal + 2,6-dichlorophenolindophenol | at about 20% of the activity as compared to 1-testosterone | Steroidobacter denitrificans | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
2-octenal + 2,6-dichlorophenolindophenol | at about 20% of the activity as compared to 1-testosterone | Steroidobacter denitrificans DSM 18526 | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
a 3-oxo-DELTA1-steroid + H2O + acceptor | overall reaction. The enzyme is specific to 3-oxo-DELTA1-steroids such as androsta-1-ene-3,17-dione and DELTA1-dihydrotestosterone and does not act on 3-oxo-DELTA4-steroids | Steroidobacter denitrificans | a steroid 1,3-dione + reduced acceptor | - |
? | |
a 3-oxo-DELTA1-steroid + H2O + acceptor | overall reaction. The enzyme is specific to 3-oxo-DELTA1-steroids such as androsta-1-ene-3,17-dione and DELTA1-dihydrotestosterone and does not act on 3-oxo-DELTA4-steroids | Steroidobacter denitrificans DSM 18526 | a steroid 1,3-dione + reduced acceptor | - |
? | |
androsta-1-ene-3,17-dione + H2O + 2,6-dichlorophenolindophenol | at about 70% of the activity as compared to 1-testosterone | Steroidobacter denitrificans | ? + reduced 2,6-dichlorophenolindophenol | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 85000 (large protein AtcA) + x * 30000 (medium protein AtcB) + x * 18000 (small protein AtcC), SDS-PAGE | Steroidobacter denitrificans |
Synonyms | Comment | Organism |
---|---|---|
1-testosterone hydratase/dehydrogenase | - |
Steroidobacter denitrificans |
ACG33_03370 | gene name, small protein AtcC | Steroidobacter denitrificans |
ACG33_03375 | gene name, medium protein AtcB | Steroidobacter denitrificans |
ACG33_03390 | gene name, large protein AtcA | Steroidobacter denitrificans |
atcABC | - |
Steroidobacter denitrificans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Steroidobacter denitrificans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8 | pH 5.5: about 30% of maximal activity, pH 8-0: about 50% of maximal activity | Steroidobacter denitrificans |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in the anaerobic degradation of steroid A-ring | Steroidobacter denitrificans |