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Literature summary for 1.14.14.139 extracted from

  • Noshiro, M.; Ishida, H.; Hayashi, S.; Okuda, K.
    Assays for cholesterol 7alpha-hydroxylase and 12alpha-hydroxylase using high performance liquid chromatography (1985), Steroids, 45, 539-550.
    View publication on PubMed
Search for more literature for 1.14.14.139

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Rattus norvegicus
-
-

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000885
-
 liver microsome Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7alpha-hydroxycholest-4-en-3-one + NADH + H+ + O2 reaction with NADH shows 15% of the activity with NADPH Rattus norvegicus 7alpha,12alpha-dihydroxycholest-4-en-3-one + NAD+ + H2O
-
 ?
7alpha-hydroxycholest-4-en-3-one + NADPH + H+ + O2 NADPH is the preferred electron source Rattus norvegicus 7alpha,12alpha-dihydroxycholest-4-en-3-one + NADP+ + H2O
-
 ?

Synonyms

Synonyms Comment Organism
HCO 12alpha-hydroxylase
-
 Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
NADH 15% of the activity with NADPH Rattus norvegicus
NADPH preferred electron source Rattus norvegicus