Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | NADPH-cytochrome P-450 reductase, EC 1.6.2.4, is required for maximal activity | Oryctolagus cuniculus |
General Stability | Organism |
---|---|
stabilized at least 4 weeks under high buffer concentrations such as 300 mM phosphate buffer | Oryctolagus cuniculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5beta-cholestane-3alpha,7alpha,12alpha-triol | 0.066 mM, 62% inhibition | Oryctolagus cuniculus | |
5beta-cholestane-3alpha,7alpha-diol | 0.066 mM, 30% inhibition | Oryctolagus cuniculus | |
Emulgen 913 | 0.1% w/v, complete inhibition | Oryctolagus cuniculus | |
MgCl2 | 1 mM, 10% inhibition | Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.027 | - |
7alpha-hydroxycholest-4-en-3-one | - |
Oryctolagus cuniculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Oryctolagus cuniculus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
non-denaturing PAGE | Oryctolagus cuniculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Oryctolagus cuniculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7alpha-hydroxycholest-4-en-3-one + NADPH + H+ + O2 | - |
Oryctolagus cuniculus | 7alpha,12alpha-dihydroxycholest-4-en-3-one + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
7alpha-hydroxy-4-cholesten-3-one 12alpha-monooxygenase | - |
Oryctolagus cuniculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.7 | 7 | - |
Oryctolagus cuniculus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 7.5 | pH 6.0: about 40% of maximal activity, pH 7.5: about 65% of maximal activity | Oryctolagus cuniculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P-450 | - |
Oryctolagus cuniculus | |
NADPH | - |
Oryctolagus cuniculus |