Literature summary for 1.1.3.21 extracted from

Streitenberger, S.A.; Lopez-Mas, J.A.; Sanchez-Ferrer, A.; Garcia-Carmona, J.A.
Highly efficient Aerococcus viridians L-alpha-glycerophosphate oxidase production in the presence of H2O2-decomposing agent: purification and kinetic characterization (2001), Appl. Microbiol. Biotechnol., 57, 329-333.

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Inhibitors

Inhibitors	Comment	Organism	Structure
benzoylformic acid	strain CETC 978, competitive inhibition	Aerococcus viridans
glyoxylic acid	strain CETC 978, competitive inhibition	Aerococcus viridans
methylglyoxal	strain CETC 978, 277 mM, complete inactivation after 50 min, in the presence of 30 mM benzoylformic acid complete inactivation after 20 min	Aerococcus viridans
additional information	strain CETC 978, not inhibited by 20-80 mM fructose-1-phosphate or fructose-6-phosphate	Aerococcus viridans

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.3	-	sn-glycerol 3-phosphate	strain CETC 978	Aerococcus viridans

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
63000	-	strain CETC 978, x * 63000, SDS-PAGE	Aerococcus viridans

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
sn-glycerol-3-phosphate + O2	Aerococcus viridans	-	glycerone phosphate + H2O2	i.e. dihydroxyacetone phosphate	?
sn-glycerol-3-phosphate + O2	Aerococcus viridans CETC 978	-	glycerone phosphate + H2O2	i.e. dihydroxyacetone phosphate	?

Organism

Organism	UniProt	Comment	Textmining
Aerococcus viridans	-	-	-
Aerococcus viridans CETC 978	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
strain CETC 978, Triton X-114, ammonium sulfate, ion-exchange chromatography, hydrophobic chromatography	Aerococcus viridans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
433.2	-	strain CETC 978	Aerococcus viridans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
sn-glycerol 3-phosphate + O2	-	Aerococcus viridans	glycerone phosphate + H2O2	-	r
sn-glycerol 3-phosphate + O2	-	Aerococcus viridans CETC 978	glycerone phosphate + H2O2	-	r
sn-glycerol-3-phosphate + O2	-	Aerococcus viridans	glycerone phosphate + H2O2	i.e. dihydroxyacetone phosphate	?
sn-glycerol-3-phosphate + O2	-	Aerococcus viridans CETC 978	glycerone phosphate + H2O2	i.e. dihydroxyacetone phosphate	?

Subunits

Subunits	Comment	Organism
?	strain CETC 978, x * 63000, SDS-PAGE	Aerococcus viridans

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	strain CETC 978	Aerococcus viridans

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Aerococcus viridans

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Release 2023.1 (January 2023)