Application | Comment | Organism |
---|---|---|
synthesis | the enzyme can be used to produce optically pure building blocks and for the bioconversion of bioactive compounds | Rhizobium leguminosarum |
Cloned (Comment) | Organism |
---|---|
gene rlgdh, recombinant overexpression of His-tagged enzyme in an active soluble form in Escherichia coli strain BL21 (DE3) | Rhizobium leguminosarum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | - |
Rhizobium leguminosarum | |
Zn2+ | - |
Rhizobium leguminosarum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Rhizobium leguminosarum | |
8.8 | - |
galactitol | pH 9.5, 35°C | Rhizobium leguminosarum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | activates | Rhizobium leguminosarum | |
Ca2+ | activates | Rhizobium leguminosarum | |
Co2+ | activates | Rhizobium leguminosarum | |
Mg2+ | required, the enzyme contains one magnesium atom per dimer, consistent with the presence of a structural metal ion. The homotetrameric enzyme contains two Mg2+ binding sites | Rhizobium leguminosarum | |
Mn2+ | activates | Rhizobium leguminosarum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
- |
Rhizobium leguminosarum |
114000 | - |
recombinant enzyme, gel filtration | Rhizobium leguminosarum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
galactitol + NAD+ | Rhizobium leguminosarum | - |
D-tagatose + NADH + H+ | - |
? | |
galactitol + NAD+ | Rhizobium leguminosarum 3841 | - |
D-tagatose + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhizobium leguminosarum | Q1MLL4 | bv. viciae | - |
Rhizobium leguminosarum 3841 | Q1MLL4 | bv. viciae | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme in an active soluble form from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography | Rhizobium leguminosarum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-butanediol + NAD+ | - |
Rhizobium leguminosarum | ? + NADH + H+ | - |
? | |
1,2-pentanediol + NAD+ | high activity | Rhizobium leguminosarum | ? + NADH + H+ | - |
? | |
1,2-propanediol + NAD+ | - |
Rhizobium leguminosarum | ? + NADH + H+ | - |
? | |
1,6-hexanediol + NAD+ | best substrate | Rhizobium leguminosarum | ? + NADH + H+ | - |
? | |
2-butanol + NAD+ | - |
Rhizobium leguminosarum | ? + NADH + H+ | - |
? | |
adonitol + NAD+ | - |
Rhizobium leguminosarum | ? + NADH + H+ | - |
? | |
D-arabinitol + NAD+ | - |
Rhizobium leguminosarum | ? + NADH + H+ | - |
? | |
D-mannitol + NAD+ | - |
Rhizobium leguminosarum | ? + NADH + H+ | - |
? | |
galactitol + NAD+ | - |
Rhizobium leguminosarum | D-tagatose + NADH + H+ | - |
? | |
galactitol + NAD+ | high activity | Rhizobium leguminosarum | D-tagatose + NADH + H+ | - |
? | |
galactitol + NAD+ | - |
Rhizobium leguminosarum 3841 | D-tagatose + NADH + H+ | - |
? | |
galactitol + NAD+ | high activity | Rhizobium leguminosarum 3841 | D-tagatose + NADH + H+ | - |
? | |
L-arabinitol + NAD+ | - |
Rhizobium leguminosarum | L-ribulose + NADH + H+ | - |
? | |
L-arabinitol + NAD+ | - |
Rhizobium leguminosarum 3841 | L-ribulose + NADH + H+ | - |
? | |
additional information | the purified recombinant RlGDH catalyzes the oxidation of a wide range of substrates, including polyvalent aliphatic alcohols and polyols, to the correspondingketones and ketoses. Among various polyols, galactitol is the preferred substrate of the enzyme. No activity with ethanol, pentanol, and propanol | Rhizobium leguminosarum | ? | - |
? | |
additional information | the purified recombinant RlGDH catalyzes the oxidation of a wide range of substrates, including polyvalent aliphatic alcohols and polyols, to the correspondingketones and ketoses. Among various polyols, galactitol is the preferred substrate of the enzyme. No activity with ethanol, pentanol, and propanol | Rhizobium leguminosarum 3841 | ? | - |
? | |
myosinitol + NAD+ | - |
Rhizobium leguminosarum | ? + NADH + H+ | - |
? | |
sorbitol + NAD+ | - |
Rhizobium leguminosarum | D-fructose + NADH + H+ | - |
? | |
xylitol + NAD+ | - |
Rhizobium leguminosarum | L-xylulose + NADH + H+ | - |
? | |
xylitol + NAD+ | - |
Rhizobium leguminosarum 3841 | L-xylulose + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 28000, recombinant enzyme, SDS-PAGE, 4 * 27289, sequence calculation | Rhizobium leguminosarum |
More | sequence alignment and secondary structure prediction, homology modeling and sequence analysis, overview | Rhizobium leguminosarum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
recombinant enzyme | Rhizobium leguminosarum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 55 | activity profile, overview | Rhizobium leguminosarum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 40 | when tested at 30°C and 35°C, the enzyme is able to retain about 50% of its activity after 114 and 54 min, respectively, while at 40°C, 50% of its activity is retained after 28 min | Rhizobium leguminosarum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13.91 | - |
galactitol | pH 9.5, 35°C | Rhizobium leguminosarum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
recombinant enzyme | Rhizobium leguminosarum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 10 | activity profile, overview. The enzyme shows over 60% of its maximum activity at pH values of 8.0-10.0 | Rhizobium leguminosarum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | the cofactor binding site in short-chain dehydrogenases/reductases enzymes is characterized by the conserved NAD-binding glycine-rich Rossmann fold domain (TGGGRAIG) | Rhizobium leguminosarum | |
NAD+ | - |
Rhizobium leguminosarum |
General Information | Comment | Organism |
---|---|---|
evolution | galactitol 2-dehydrogenase belongs to the protein subfamily of short-chain dehydrogenases/reductases | Rhizobium leguminosarum |
additional information | homology modeling and sequence analysisA , overview. The active site contains residues Asn119,Ser147, Tyr162, and Lys166 which are conserved in short-chain dehydrogenases/reductases | Rhizobium leguminosarum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.581 | - |
galactitol | pH 9.5, 35°C | Rhizobium leguminosarum |